A12-Iso5-4DC19 - ≥98% , CAS No.2412492-20-5

CAS: 2412492-20-5 Cat. No.: A1440319 Formula: C₄₇H₈₅N₃O₂ Molecular Weight: 724.20

GRADE & PURITY ≥98%

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

A12-Iso5-4DC19 is an ionizable cationic lipid. A12-Iso5-4DC19 can facilitate mRNA delivery and be used for the research of RNA vaccines.

Specifications

Specifications & Purity

≥98%

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥98%

Names and Identifiers

Molecular Weight

724.20

Application Protocols

No validated application protocols are provided for this item in the Product Data.

General guidance for screening compounds

• Prepare fresh DMSO stocks as needed, record exact concentration, and minimize freeze–thaw by aliquoting.
• Include vehicle controls matching final solvent percentages in assay wells.
• Employ serial dilutions (e.g., 3- or 10-fold) for dose–response curves, ensuring compound remains in solution across the range.
• Use orthogonal readouts where possible to exclude assay interference.

Refer to your lab’s SOPs and the product CoA/SDS for any item-specific instructions.

Biological Roles

Biochemical/biological roles for A12-Iso5-4DC19 are not specified in the Product Data.

General considerations for library compounds

• Potential activities may span enzyme inhibition/activation, receptor modulation, or pathway perturbation; definitive roles require empirical screening and target deconvolution.
• Implement orthogonal assays to exclude assay interference mechanisms (fluorescence quenching, redox cycling, PAINS-like behavior). Use counterscreens and detergent-based aggregation controls.
• If a putative target emerges, perform dose–response analysis, determine basic ADME-relevant properties (solubility, stability in buffer/media, permeability in PAMPA/MDCK—if applicable), and evaluate selectivity across related proteins.

No biological claims are made for this product. It is supplied strictly for research use only.

Buffer Applications

Not typically applicable. This product is a small-molecule library member, not a buffering agent. If the compound will be used in biochemical assays, prepare in assay-compatible buffers and maintain low co-solvent content. Refer to your assay protocol for buffer composition and pH control.

Green Alternatives

Item-specific solvent and process data are not provided; suggestions below are general best practices for small-molecule handling.

• Prefer greener solvents for dilutions and workups when compatible: water, ethanol, isopropanol, and ethyl acetate often present lower environmental/health impacts compared with DMF, NMP, or dichloromethane (general guidance).
• When DMSO is required for stocks, keep final assay solvent percentages as low as feasible (≤0.5–1%) to reduce solvent load.
• Use microscale formats and high-throughput dispensing to minimize solvent consumption and waste.
• Opt for energy-efficient operations: ambient-temperature workflows when stability allows; avoid excessive heating/cooling.

Illustrative comparison (general)

• DMF/NMP vs. EtOAc/MeCN: DMF/NMP dissolve broader chemotypes but have higher toxicity and persistence; MeCN and EtOAc are more benign but may not dissolve very lipophilic scaffolds.
• Halogenated solvents: Avoid chloroform/DCM unless strictly necessary for analytics; consider cyclopentyl methyl ether or 2-MeTHF for apolar needs when compatible with the compound and assay.

Confirm compatibility with your assay system and consult the CoA/SDS for any item-specific restrictions.

Pharmaceutical Uses

No pharmacopeial status or excipient role is provided. This product is offered for research use only and is not intended for human or veterinary use, clinical diagnostics, or as a drug substance. For any formulation feasibility studies, obtain full structural and stability data first and perform standard preformulation assessments (solubility, pH stability, light sensitivity) under research conditions only.

Physical Properties

Item-specific physicochemical values are not supplied in the Product Data.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• pKa / logP: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.

Practical guidance (general)

• For small-library members of unknown structure, initial solubility screening typically starts with DMSO at 10–50 mM, then dilution into aqueous buffers containing co-solvent (e.g., 0.1–1% DMSO) as assay permits (general practice).
• If hygroscopic or poorly soluble, gentle warming (≤40 °C) and brief sonication can aid dissolution (general practice). Avoid strong base/acid until the functional groups are known.

Always rely on the item’s CoA/SDS for definitive specifications.

Quality and Grades

The Product Data do not specify a grade, purity, or stabilizer for A12-Iso5-4DC19.

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.
• Stabilizers/antioxidants: Not specified for this item; refer to CoA/Spec Sheet.

Guidance on library compound quality (general)

• Research-use small molecules are typically provided with an assay of purity (e.g., HPLC area %) and identity (MS/HRMS; sometimes NMR). Verify acceptance criteria on the CoA before critical experiments.
• If used in screening, request or record lot-specific purity and analytical chromatograms to ensure reproducibility.
• UV background and counter-screening: For optical assays, low UV/visible absorbance in the assay window is desirable; check spectral data if provided.
• Water/peroxide/metal limits: Not specified for this item; refer to CoA/Spec Sheet. Do not assume trace impurity limits without documentation.

Recommendations

• If dry solid: record mass on a calibrated balance and track compound inventory with lot/aliquoting to reduce freeze–thaw and moisture uptake.
• If supplied in solution: verify solvent, concentration, and stabilizers on the label/CoA; store under the stated conditions and avoid evaporative concentration unless stability is known.

Reaction and Applications

This product is positioned as a small-molecule library member rather than a synthetic reagent. Without a disclosed structure, reaction planning or named-reaction participation cannot be specified.

Practical research applications (general for library compounds)

• Hit identification and profiling: Use in biochemical/biophysical screens (enzyme assays, binding assays, phenotypic cell-based assays) with appropriate controls and counter-screens.
• Analytical characterization: Confirm identity/purity upon receipt via LC–MS and, if feasible, 1H NMR to verify lot integrity before screening campaigns.
• Stability checks: Conduct short-term stability and freeze–thaw assessments in the chosen solvent (e.g., DMSO) to avoid degradation during screening.

Notes

• If you intend to derivatize or use this molecule synthetically, obtain full structural information and reactivity data from the CoA/Spec Sheet prior to designing reactions, including protecting-group strategy and compatibility with catalysts or reagents.
• For mechanistic biology studies, consider orthogonal assays (thermal shift, SPR/ITC, CETSA) and check for aggregation or redox activity using appropriate controls (e.g., detergent sensitivity, redox-cycling assays).

Reaction Conditions

Not applicable in the absence of a disclosed structure. No general reaction conditions can be recommended for this item as a reagent.

If using the compound in assays rather than synthesis, focus on solution preparation conditions:

• Typical stock: Dissolve in an appropriate solvent (often DMSO for screening compounds) at a convenient concentration (e.g., 10–50 mM), filter if needed through 0.2 µm PTFE, and store cold per stability guidance (general practice). Adjust according to CoA/SDS when available.

Safety and Handling

GHS/SDS details are not provided for this specific item; consult the product SDS for authoritative information.

• GHS classification: Not specified for this item; refer to SDS.
• Signal word / pictograms / H-statements: Not specified for this item; refer to SDS.

General lab safety (applicable to unknown small molecules)

• PPE: Wear lab coat, safety glasses, and appropriate chemically resistant gloves (e.g., nitrile). Use in a fume hood to avoid inhalation of vapors or dusts.
• Storage: Store at 2–8 °C (per Product Data). Keep container tightly closed. Protect from moisture and light until structural sensitivity is known. Avoid freezing unless the CoA/SDS indicates stability.
• Incompatibilities: Unknown; segregate from strong acids/bases and strong oxidizers as a precaution until specific reactivity is known.
• Handling: Minimize exposure and avoid aerosol/dust generation. If in solution, confirm solvent compatibility with plastics/elastomers.
• First aid (overview; defer to SDS): Inhalation—move to fresh air; Skin/eye—rinse with water for at least 15 minutes; Ingestion—rinse mouth. Seek medical attention and provide SDS.
• Spill response: Absorb with inert material, collect in chemical waste. Decontaminate area and ventilate.

Waste disposal

• Dispose in accordance with institutional and local regulations for organic chemical waste. Do not release to drains.

Solvent Selection

Because structure and polarity are not disclosed, solvent choice should be established empirically for this library member.

General starting points (common for diverse screening compounds)

• Primary stock solvent: DMSO is preferred due to broad solvating power and assay compatibility. Begin at 10–50 mM, then dilute into assay buffer to ≤0.5–1% DMSO final as protocol allows (general practice).
• Alternative high-permittivity solvents: DMF, NMP (handle with care; assess toxicity and material compatibility). For less polar scaffolds, consider MeCN, EtOAc, or IPA for intermediate processing steps.
• Aqueous compatibility: Use co-solvent systems (buffer + 0.1–1% DMSO or MeCN). Add solvent to buffer first, then compound, with gentle mixing to minimize precipitation.
• Limited solubility: Employ small amounts of co-solvents, surfactants (e.g., 0.01–0.05% Tween 80 if biologically acceptable), or pH adjustment only when functional groups and stability are known.

Comparison (general)

• DMSO: highest success rate for initial dissolution; hygroscopic and high bp.
• MeCN: volatile, miscible with water, lower viscosity; less universal solubility.
• EtOH/IPA: assay-friendly at low percentages but may be less effective for highly lipophilic compounds.

Always confirm solvent compatibility with your assay/analytical platform and consult the CoA/SDS for any item-specific instructions.

Storage and Reconstitution

• Storage conditions (from Product Data): Store at 2–8 °C. Keep tightly closed. Protect from moisture and light until structural sensitivity is known.
• Shipping: Shipped on wet ice (from Product Data). Inspect upon receipt and return to recommended storage immediately.

General reconstitution guidance for small-molecule library items

• Solids: Bring to room temperature in a desiccator before opening to prevent condensation. Dissolve in a suitable solvent (commonly DMSO for initial stocks). Aim for 10–50 mM stocks unless solubility dictates otherwise. If needed, gently warm (≤40 °C) and briefly sonicate. Document exact solvent and concentration.
• Solutions: Verify label concentration and solvent. Mix gently after thawing/cooling; avoid repeated freeze–thaw by aliquoting.
• Filtration: If particulate is observed, sterile-filter through 0.2 µm PTFE (chemical compatibility dependent on solvent).
• Stability: Item-specific stability, hygroscopicity, and light sensitivity are Not specified for this item; refer to CoA/Spec Sheet. Until known, minimize light/air exposure and avoid prolonged room-temperature storage.
• Research use only: Not for human or veterinary use.

Dispose of unused material and solutions per institutional chemical waste procedures.

Structure and Identity

This is a cataloged small-molecule library member. Item-specific structural identifiers are not disclosed in the provided data.

• SKU: A1440319
• Product Name: A12-Iso5-4DC19
• CAS: 2412492-20-5
• Category: Small molecules and compound libraries (research use)
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Structural features

• 2D/3D structure, functional groups, stereochemistry: Not specified for this item; refer to CoA/Spec Sheet.

Notes

• Without a disclosed structure, discussion of ring systems, heteroatoms, or stereocenters is not possible. If you have an internal structure key for this library member, align it with the CAS above to retrieve full identifiers before use in computational or synthetic workflows.

Synthetic Utility

Not assessable without the disclosed chemical structure. If you plan synthetic derivatization or SAR expansion:

• Obtain the full structure, functional-group profile, and any protecting groups from the CoA/Spec Sheet.
• Map potential liabilities (acid/base sensitivity, redox-active motifs, peroxidizable moieties) before route design.
• Confirm availability of analytical standards and consider reference spectra (NMR, MS) for intermediate tracking.

Until structure is available, no claims can be made regarding named reactions, orthogonal protecting strategies, or retrosynthetic disconnections.

Target Specificity

No target, epitope, or selectivity information is provided for this small-molecule library member.

• Target class, species reactivity, and mechanism of action: Not specified for this item; refer to CoA/Spec Sheet or internal screening data.

For discovery work, determine specificity via panel screening against related proteins, off-target profiling, and orthogonal binding/functional assays.

Frequently Asked Questions

What is the purity of this product?

This product is supplied at ≥98% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2412492-20-5, the molecular formula is C47H85N3O2, and the molecular weight is 724.20 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.