Actinomycin D (Dactinomycin) - 10mM in DMSO , CAS No.50-76-0

CAS: 50-76-0 Cat. No.: A408625 Formula: C₆₂H₈₆N₁₂O₁₆ Molecular Weight: 1255.42 EC Number: 200-063-6

GRADE & PURITY 10mM in DMSO

Synonyms

Act D, RASP-101 | Actinomycin D

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs,FedEx DG Service

Overview

Information

Actinomycin D (Dactinomycin) Actinomycin D (Dactinomycin, Act D, RASP-101) is a significant polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. Actinomycin D (Dactinomycin) inhibits DNA repair and rests the cell cycle at G1 pha
In vitro

Actinomycin D markedly reduces the SMC proliferation via the inhibition of BrdU incorporation at 80 nM. This is further supported by the G1-phase arrest using a flowcytometric analysis. The protein expression levels of proliferating cell nuclear antigen (PCNA), focal adhesion kinase (FAK), and Raf are all suppressed by actinomycin D. Extracellular signalregulated kinases (Erk) involved in cell-cycle arrest are found to be increased by actinomycin D.

In vivo

Actinomycin D targets survival proteins TOSO, BCL2 and MCL1 and is active in two different mouse models that are characterized by either unmutated B-cell receptor or inactive p53 function, both of which are known negative prognostic factors in CLL.
Cell Data

cell lines：Ba/F3 cells

Concentrations：80 nM, 800 nM, 8 μM

Incubation Time：18-24 h

Powder Purity:≥98%

Specifications

Synonyms

Act D, RASP-101 | Actinomycin D

Specifications & Purity

10mM in DMSO

Biochemical and Physiological Mechanisms

Actinomycin D (Dactinomycin, Act D, RASP-101) is a significant polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. Actinomycin D (Dactinomycin) inhibits DNA repair and rests the cell cycle at G1 phase with IC50 of 0.42 μM and 0.4

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs,FedEx DG Service

This product requires cold chain shipping. Ground and other economy services are not available.

Product Properties

alogp

3.8

Names and Identifiers

Isomeric SMILES

C[C@@H]1[C@@H](C(=O)N[C@@H](C(=O)N2CCC[C@H]2C(=O)N(CC(=O)N([C@H](C(=O)O1)C(C)C)C)C)C(C)C)NC(=O)C3=C4C(=C(C=C3)C)OC5=C(C(=O)C(=C(C5=N4)C(=O)N[C@H]6[C@H](OC(=O)[C@@H](N(C(=O)CN(C(=O)[C@@H]7CCCN7C(=O)[C@H](NC6=O)C(C)C)C)C)C(C)C)C)N)C

UN Number

2811

Packing Group

I

Molecular Weight

1255.42

Reaxy-Rn

1071183

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=1071183&ln=

Chemical and Physical Properties

• x_melt_point_char: 251-253°C