10mM in DMSO for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs,FedEx DG Service Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 48 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Information
Actinomycin D (Dactinomycin) Actinomycin D (Dactinomycin, Act D, RASP-101) is a significant polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. Actinomycin D (Dactinomycin) inhibits DNA repair and rests the cell cycle at G1 pha
In vitro
Actinomycin D markedly reduces the SMC proliferation via the inhibition of BrdU incorporation at 80 nM. This is further supported by the G1-phase arrest using a flowcytometric analysis. The protein expression levels of proliferating cell nuclear antigen (PCNA), focal adhesion kinase (FAK), and Raf are all suppressed by actinomycin D. Extracellular signalregulated kinases (Erk) involved in cell-cycle arrest are found to be increased by actinomycin D.
In vivo
Actinomycin D targets survival proteins TOSO, BCL2 and MCL1 and is active in two different mouse models that are characterized by either unmutated B-cell receptor or inactive p53 function, both of which are known negative prognostic factors in CLL.
Cell Data
cell lines：Ba/F3 cells
Concentrations：80 nM, 800 nM, 8 μM
Incubation Time：18-24 h
Powder Purity:≥98%
| ALogP | 3.8 |
|---|
| Isomeric SMILES | C[C@@H]1[C@@H](C(=O)N[C@@H](C(=O)N2CCC[C@H]2C(=O)N(CC(=O)N([C@H](C(=O)O1)C(C)C)C)C)C(C)C)NC(=O)C3=C4C(=C(C=C3)C)OC5=C(C(=O)C(=C(C5=N4)C(=O)N[C@H]6[C@H](OC(=O)[C@@H](N(C(=O)CN(C(=O)[C@@H]7CCCN7C(=O)[C@H](NC6=O)C(C)C)C)C)C(C)C)C)N)C |
|---|---|
| UN Number | 2811 |
| Packing Group | I |
| Molecular Weight | 1255.42 |
| Reaxy-Rn | 1071183 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=1071183&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Melt Point(°C) | 251-253°C |
|---|
| 1. Cen Junjie, Zhao Dongliang, Shi Xin, Chen Jinlong, Zhou Hang, Liang Yanping, Gui Chengpeng, Chen Wei, Luo Junhang, Chen Xu. (2025) N6-methyladenosine-mediated upregulation of MANF promotes ER stress resistance in renal cell carcinoma. Cell Death & Disease, 16 (1): (1-15). [PMID:40603319] [10.1038/s41419-025-07798-4] |
| 2. Wang Liping, Tao Chenyue, Chen Shuai, Gui Liu, Zhang Yitong, Jiang Min, Zhou Dongjie, Zhou Luojing. (2025) circRNA_BMPR2 affects the progression of polycystic ovary syndrome by regulating the expression of Rab43. GYNECOLOGICAL ENDOCRINOLOGY, [PMID:40601689] [10.1080/09513590.2025.2526559] |
| 3. Dou Yufeng, Yang Jiao, Huang Na, Lu Xiaoxiao, Yan Liping. (2025) Quercetin alleviates LPS-induced embryonic lung fibroblast injury by regulating METTL3/TBL1XR1 expression. Applied Biological Chemistry, 68 (1): (1-11). [PMID:] [10.1186/s13765-025-01036-3] |
| 4. Wei Liu, Hui Zeng. (2023) IGF2BP2 attenuates intestinal epithelial cell ferroptosis in colitis by stabilizing m6A-modified GPX4 mRNA. CYTOKINE, [PMID:38039694] [10.1016/j.cyto.2023.156388] |
| 5. Fan Hairui, Zhou Dongjie, Zhang Xiaomei, Jiang Min, Kong Xiang, Xue Tongmin, Gao Lingling, Lu Dan, Tao Chenyue, Wang Liping. (2023) hsa_circRNA_BECN1 acts as a ceRNA to promote polycystic ovary syndrome progression by sponging the miR-619-5p/Rab5b axis. MOLECULAR HUMAN REPRODUCTION, 29 (11): [PMID:37882757] [10.1093/molehr/gaad036] |
| 6. Chen Guo, Mingyi Yu, Jinghua Liu, Zhe Jia, Hui Liu, Shaozhen Zhao. (2023) Molecular mechanism of Wilms tumor 1-associated protein in diabetes-related dry eye disease by mediating m6A methylation modification of lncRNA NEAT1. JOURNAL OF DRUG TARGETING, [PMID:38153328] [10.1080/1061186X.2023.2300682] |
| 7. Zhang Yang, Chao Fengmei, Lv Lihua, Li Ming, Shen Zuojun. (2023) Hsa_circ_0041150 serves as a novel biomarker for monitoring chemotherapy resistance in small cell lung cancer patients treated with a first-line chemotherapy regimen. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, [PMID:37639013] [10.1007/s00432-023-05317-6] |
| 8. Wei-Hang Zhu, Jie Chen, Run-Kai Huang, Yuan Zhang, Ze-Xuan Huang, Xiu-Qing Pang, Bo Hu, Yang Yang, Xing Li. (2023) Erythroid-transdifferentiated myeloid cells promote portal vein tumor thrombus in hepatocellular carcinoma. Theranostics, [PMID:37649603] [10.7150/thno.82907] |
| 9. Jing Zhang, Kairu Liu, Xiaojuan Duan, Xuhao Wang, Wupeng Ge, Wenping Jin. (2023) Restoration of Choujiu Koji and evaluation of its brewing performance. LWT-FOOD SCIENCE AND TECHNOLOGY, [PMID:] [10.1016/j.lwt.2023.114933] |
| 10. Fei Sun, Cheng An, Can Liu, Ying Hu, Yue Su, Zhixiang Guo, Hong Che, Shenglin Ge. (2023) FTO represses NLRP3-mediated pyroptosis and alleviates myocardial ischemia–reperfusion injury via inhibiting CBL-mediated ubiquitination and degradation of β-catenin. FASEB JOURNAL, 37 (6): (e22964). [PMID:37199660] [10.1096/fj.202201793RR] |
| 11. Zhiyong Guo, Zilin Wang, Yige Liu, Hao Wu, Qiaoyu Zhang, Jing Han, Jiannan Liu, Chenping Zhang. (2023) Carbon Dots from Lycium barbarum Attenuate Radiation-Induced Bone Injury by Inhibiting Senescence via METTL3/Clip3 in an m6A-Dependent Manner. ACS Applied Materials & Interfaces, [PMID:37088945] [10.1021/acsami.3c01322] |
| 12. Yang Liu, Du Xing, Wang Siqi, Lin Chenggang, Li Qiqi, Li Qifa. (2023) A regulatory network controlling ovarian granulosa cell death. Cell Death Discovery, 9 (1): (1-11). [PMID:36806197] [10.1038/s41420-023-01346-9] |
| 13. Zhang Shouyi, Lv Chengcheng, Niu Yichen, Li Changqi, Li Xiuming, Shang Yu, Zhang Yunchao, Zhang Yue, Zhang Yong, Zeng Yu. (2023) RBM3 suppresses stemness remodeling of prostate cancer in bone microenvironment by modulating N6-methyladenosine on CTNNB1 mRNA. Cell Death & Disease, 14 (2): (1-14). [PMID:36750551] [10.1038/s41419-023-05627-0] |
| 14. Lifang Liu, Bingkai Han, Lixia Liu, Hongying Cui, Hao Liu, Rui Jia, Xiaoyan Zhang, Xiaoxiao Lu. (2022) Circ_0021573 acts as a competing endogenous RNA to promote the malignant phenotypes of human ovarian cancer cells. Reproductive Biology, [PMID:36481473] [10.1016/j.repbio.2022.100704] |
| 15. Jin-Xin Yu, Yan-Min Hui, Jun-Ao Xue, Jia-Bao Qu, Si-Quan Ling, Wei Wang, Xin-Nian Zeng, Jia-Li Liu. (2022) Formation characteristics of long-term memory in Bactrocera dorsalis. Insect Science, 30 (3): (829-843). [PMID:36151856] [10.1111/1744-7917.13119] |
| 16. Jiayu Lin, Yuting Xiang, Jiana Huang, Haitao Zeng, Yanyan Zeng, Jiawen Liu, Taibao Wu, Qiqi Liang, Xiaoyan Liang, Jingjie Li, Chuanchuan Zhou. (2022) NAT10 Maintains OGA mRNA Stability Through ac4C Modification in Regulating Oocyte Maturation.. Frontiers in Endocrinology, [PMID:35937804] [10.3389/fendo.2022.907286] |
| 17. Peng Qinying, Gao Guixi, Lü Jin, Long Qingshan, Chen Xuefei, Zhang Fei, Xu Min, Liu Kai, Wang Yemin, Deng Zixin, Li Zhiyong, Tao Meifeng. (2018) Engineered Streptomyces lividans Strains for Optimal Identification and Expression of Cryptic Biosynthetic Gene Clusters. Frontiers in Microbiology, [PMID:30619133] [10.3389/fmicb.2018.03042] |
| 18. Longchen Yu, Qining Guo, Yaping Li, Mai Mao, Zhenping Liu, Tingting Li, Lei Wang, Xin Zhang. (2025) CHMP4C promotes pancreatic cancer progression by inhibiting necroptosis via the RIPK1/RIPK3/MLKL pathway. Journal of Advanced Research, [PMID:39870301] [10.1016/j.jare.2025.01.040] |
| 19. Gengchen Xie, Bo Lei, Zhijie Yin, Fei Xu, Xinghua Liu. (2024) CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2 Degradation via Interacting with UCHL3. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 25 (5): (2817). [PMID:38474064] [10.3390/ijms25052817] |
| 20. Jun Weng, Geng Miaomiao, Xiaoyu Hu, Yang Hu, Yi Yang, Banbin Xing, Wu Zhiyong, Zigong Wei. (2024) Design of minibinder proteins specific to TNFR1. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:39743082] [10.1016/j.ijbiomac.2024.139403] |
| 21. Jinxin Yu, Jiayi He, Xuefeng Zhang, Chuxiao Lin, Shiyan Liu, Xin Gong, Xinnian Zeng, Jiali Liu. (2024) Differential energy pathways are required for rapid long-term memory formation in the oriental fruit fly, Bactrocera dorsalis. Journal of Integrative Agriculture, [PMID:] [10.1016/j.jia.2024.12.015] |
| 22. Xing Fa-liang, Li Bo-rui, Fang Ying-jin, Liang Chen, Liu Jiang, Wang Wei, Xu Jin, Yu Xian-jun, Qin Yi, Zhang Bo. (2024) G3BP2 promotes tumor progression and gemcitabine resistance in PDAC via regulating PDIA3-DKC1-hENT in a stress granules-dependent manner. ACTA PHARMACOLOGICA SINICA, [PMID:39289547] [10.1038/s41401-024-01387-5] |
| 23. Wenxian Tu, Meina Feng, Qin Zhou, Yunfeng Wang, Mingye Wan, Danqun Gong, Jin Li, Yuanmin Du. (2024) GATA2‑miR‑374a axis promotes vascular smooth muscle cells proliferation, migration via targeting circTADA2A/RORA axis. Experimental and Therapeutic Medicine, 28 (3): (1-11). [PMID:39071901] [10.3892/etm.2024.12646] |
| 24. Huihui An, Yifan Hong, Yeek Teck Goh, Casslynn W.Q. Koh, Shahzina Kanwal, Yi Zhang, Zhaoqi Lu, Phoebe M.L. Yap, Suat Peng Neo, Chun-Ming Wong, Alice S.T. Wong, Yang Yu, Jessica Sook Yuin Ho, Jayantha Gunaratne, Wee Siong Sho Goh. (2024) m6Am sequesters PCF11 to suppress premature termination and drive neuroblastoma differentiation. MOLECULAR CELL, [PMID:39481383] [10.1016/j.molcel.2024.10.004] |
| 25. Xiaoshan Wang, Mengding Chen, Yuwei Wu, Feixu Chen, Tong Shen, Zhengguang Wang. (2025) RORα inhibits proliferation and chemoresistance through AKR1A1-induced glucose and lipid reprogramming in gastric cancer. CELLULAR SIGNALLING, [PMID:40096932] [10.1016/j.cellsig.2025.111741] |
| 26. Zheng Wang, Mingyu Zhao, Yue Su, Qiangsen Zhao, Zhenhua Ma, Qiaoxian Yue, Zhiwei Zhu, Lihuan Zhang, Zhuocheng Hou, Huifeng Li. (2024) The impact of NUMB on chicken abdominal adipogenesis: A comprehensive analysis. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:39168214] [10.1016/j.ijbiomac.2024.134904] |
| 27. N.I.N.G. HAN, N.A. YU, L.I. YU. (2024) The mRNA stability of PIEZO1, regulated by METTL3 via m6A modification in a YTHDF2-dependent manner, facilitates the progression of diabetic retinopathy. AMERICAN JOURNAL OF PATHOLOGY, [PMID:39476953] [10.1016/j.ajpath.2024.10.007] |
| 28. He Chaojie, Fan Hongyan, Zhu Chunyan, Huang Yueqing. (2025) The up-regulation of ANXA1 by FTO-dependent demethylation alleviates NLRP3-mediated pyroptosis and inflammation in myocardial ischemia–reperfusion injury. Molecular & Cellular Toxicology, [PMID:] [10.1007/s13273-025-00513-6] |
| 29. Hui Gan, Mi Zhang, Yuhao Duan, Palahati Ailiyaer, Qi He, Junyi Tan, Yong Li, Xuan Zhai, Jing Zhao. (2025) Microglial NFAT5 aggravates neuroinflammation via mediating NLRP6 inflammasome in experimental ischemic stroke. Genes & Diseases, [PMID:40831534] [10.1016/j.gendis.2025.101614] |
| 30. Wu Zhiwei, Zhang Fan, Yang Kai, He Wenfei. (2025) CircSCD1 inhibits ferroptosis in breast Cancer through stabilizing SCD1 protein via deubiquitinase OTUB1. Scientific Reports, 15 (1): (1-12). [PMID:40210941] [10.1038/s41598-025-96868-w] |
| 31. Kang Li, Yuxuan Yi, Rongsong Ling, Caihua Zhang, Zhihui Zhang, Yue Wang, Ganping Wang, Jie Chen, Maosheng Cheng, Shuang Chen. (2025) PCIF1 drives oesophageal squamous cell carcinoma progression via m6Am-mediated suppression of MTF2 translation. Clinical and Translational Medicine, 15 (4): (e70286). [PMID:40156159] [10.1002/ctm2.70286] |
| 32. Jing Zhang, Xuewei Yang, Xing Zhao, Gang Luo, Hong Jiang. (2025) KLF3 aggravates renal fibrosis in chronic kidney disease through transcriptional activation of DDAH2. BIOCHEMICAL PHARMACOLOGY, [PMID:40480524] [10.1016/j.bcp.2025.117033] |
| 33. Maoxin Huang, Liping Dong, Yue He, Xinying Cai, Yun Lu, Jinlei Xu, Fengli Xiao. (2025) YTHDF1 regulates the proliferation and differentiation of keratinocytes via the PI3K/AKT signaling pathway in atopic dermatitis. ALLERGOLOGY INTERNATIONAL, [PMID:40483223] [10.1016/j.alit.2025.04.005] |
| 34. Cai Zhiren, Xie Yanqi, Li Luyao, Zheng Yuhang, Zhou Xianghui, Zhou Guanghua, Wang Ganping, Zeng Xin. (2025) TET3 facilitates bladder cancer progression through targeted modulation of stemness pathways. MEDICAL ONCOLOGY, 42 (8): (1-13). [PMID:40569530] [10.1007/s12032-025-02857-4] |
| 35. Yu Zhang, Xiaojie Liu, Hairong Lian, Yanchao Chai, Yang Zhou, Dongqi Kan, Jilong Ren, Cui Han, Jiaxin Yang. (2025) Metformin and Dietary Restriction Counteract Aging via Reducing m6A–Dependent Stabilization of Methionine Synthase mRNA in Brachionus asplanchnoidis (Rotifera). AGING CELL, [PMID:40424068] [10.1111/acel.70113] |
| 36. Jie Hu, Qilong Liu, Bi Feng, Yanling Lu, Kai Chen. (2025) ALYREF-Mediated Regulation of TBL1XR1 and KMT2E Synergistically Upregulates APOC1, Contributing to Oxaliplatin Resistance in Esophageal Cancer. BREAST CANCER RESEARCH AND TREATMENT, 57 (4): (1064). [PMID:39901699] [10.4143/crt.2024.1091] |
| 37. Mingxing Sui, Shuying Wu, Qingqing Song, Siyao Chen, Yingli Lu. (2025) FOXO6, Stabilized by METTL3-Mediated m6A Modification, Accelerates Polycystic Ovary Syndrome Progression by Transcriptional Activation of TXNIP. FASEB JOURNAL, 39 (20): (e71141). [PMID:41091548] [10.1096/fj.202502357R] |
| 38. Li Yuanbin, Feng Chuanping, Lin Hui, Li Hui, Zeng Wei, Dong Xiaoyan, Chen Zhuang. (2025) Protective role of HOXB5-orchestrated mitochondrial homeostasis and mitophagy in cardiomyocytes after ischemia-reperfusion via transcriptionally activating Sirt5. CARDIOVASCULAR DRUGS AND THERAPY, [PMID:41182565] [10.1007/s10557-025-07785-z] |
| 39. Ji Kai, Zhou Leqi, Zhang Tianshuai, Fan Hao, Xie Gang, Man Wenjiang, Wang Can, Tian Yucheng, Chen Li, Wang Guimin, Liu Mulin, Zhu Bing, Zhang Wei, Yu Guanyu. (2025) hnRNP A1 inhibits colorectal cancer tumorigenesis and progression by regulating fatty acid metabolism and RNA stability. Cell Death Discovery, 11 (1): (542). [PMID:41285764] [10.1038/s41420-025-02814-0] |
| 40. Zeming Lei, Yansheng Wang, Liangzi Yin, Ziyuan Wang, Lunhao Bai. (2025) Periprosthetic osteolysis is mediated by the m6A-dependent regulation of CEMIP via YTHDF2. INTERNATIONAL IMMUNOPHARMACOLOGY, [PMID:41308363] [10.1016/j.intimp.2025.115895] |
| 41. Chun-Xue Zhou, Si-Qi Wang, Ji-Yu Zhang, Xing Du, Qi-Fa Li. (2025) HNRNPA2B1-mediated m6A modification enhances lncRNA NORHA stability to control granulosa cell functions. ZOOLOGICAL RESEARCH, 46 (3): (722). [PMID:40407136] [10.24272/j.issn.2095-8137.2024.378] |
| 42. Mingxing Sui, Shuying Wu, Qingqing Song, Siyao Chen, Yingli Lu. (2026) Onecut2, negatively regulated by methyltransferase METTL3, inhibits polycystic ovary syndrome by transcriptionally modulating SIRT3 expression. BIOCHEMICAL PHARMACOLOGY, [PMID:41544857] [10.1016/j.bcp.2026.117712] |
| 43. Mao Dong, Sun Jufeng, Zhang Xiaowei, Wang Zhaoping, Zhang Yun. (2026) ILF2 cooperates with ILF3/KLF16 to drive colorectal cancer progression via modulating the behaviors of both tumor cells and M2 macrophages. APOPTOSIS, 31 (2): (52). [PMID:41572068] [10.1007/s10495-026-02259-5] |
| 44. Dongsheng Xing, Hongwei Cao, Yan Yang, Shengyang Liu, Hanbing Yu, Zhenyu Liu, Kunrong Wang, Xin Wei, Aihui Yan. (2026) RNA-binding protein tristetraprolin inhibits Th2 cell activation and differentiation in allergic rhinitis by promoting TRIM18 mRNA decay. JOURNAL OF BIOLOGICAL CHEMISTRY, [PMID:41651421] [10.1016/j.jbc.2026.111240] |
| 45. Huanhuan Wang, Hanshu Zhao, Jiadi Gao, Sihan Liu, Yuanqi Xu, Xinyue Zhao, Shanshan Yang, Baozhi Gang, Zhongling Zhang. (2026) METTL14/m6A/CEBPD Axis: A Critical Player in Chronic Cerebral Hypoperfusion–Associated Neuroinflammation. FREE RADICAL BIOLOGY AND MEDICINE, [PMID:41791683] [10.1016/j.freeradbiomed.2026.03.013] |
| 46. Li Ze, Ling Yimin, Xiao Wei, Li JiaZheng, Rong JiaHui, Zhang Zewen, Wei Yan, Huo Zhongchao, Li Dong. (2026) Scutellarin mediates cell cycle arrest and apoptosis in liver cancer through a novel circSRBD1/miR-2682-5p/ESR1 ceRNA axis. Journal of Translational Medicine, 24 (1): (492). [PMID:] [10.1186/s12967-026-07931-7] |
| 47. Han Tianci, Xie Junwei, Tong Wei, Zhang Liang. (2026) ID4 suppresses proliferation and macrophage polarization in esophageal carcinoma through interaction with TCF4. MOLECULAR CANCER RESEARCH, [PMID:41770101] [10.1158/1541-7786.MCR-25-0686] |
| 48. Weijian Lun, Yongjia Liu, Canhua Luo, Huihuan Wu. (2026) ZNF460 Promotes the m6A Modification of COPS6 to Facilitate Immune Escape in Colorectal Cancer. FASEB JOURNAL, 40 (6): (e71640). [PMID:] [10.1096/fj.202503485R] |
