α-Amylase, CAS No.9000-90-2

CAS: 9000-90-2 Cat. No.: A109181 EC Number: 232-565-6

GRADE & PURITY EnzymoPure™, from Bacillus amyloliquefaciens; ≥480 KNU-B/g

Accession #

P00692

Bioactivity

≥480 KNU-B/g

Overview

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. This product is from Bacillus amyloliquefaciens and is supplied as a liquid. α-Amylase has been used in various plant studies, such as metabolism studies in Arabidopsis. α-Amylase from Bacillus amyloliquefaciens has been used to hydrolyze sweetpotato amylopectin to identify cluster structure. The enzyme has been used to hydrolyze sago palm starch to reducing sugars, which are then used for ethanol fermentation by Saccharomyces cerevisiae FNCC 3012. The enzyme catalyzes amylolysis of gelatinised waxy maize starch to produce reducing sugars.

Biochem/physiol Actions

α-Amylase is an endoamylase that randomly hydrolyzes the α-(1,4) glucan linkages in amylose and amylopectin. The breakdown products are oligosaccharides and dextrins of varying chain length. This enzyme works best at high temperatures, in the range of 70-90 ° Celcius.

An endoamylase that randomly hydrolyzes α-(1→4)-glycosidic linkages in amylose and amylopectin. The breakdown products are oligosaccharides and dextrins of varying chain length. This enzyme is active at high temperatures (70−90 °C).

The pH range for the α-amylase of B.amyloliquefaciens is 5.5 to 6.5, with a single maximum at 5.9. The activity is rapidly inactivated below pH 5.0.

Specifications

Product Name

α-Amylase, CAS No.9000-90-2

Synonyms

1,4-α-D-Glucan-glucanohydrolase | 1,4-alpha-D-Glucan-glucanohydrolase | alpha-amylase | glycogenase | mesotherm α-Amylase

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™,from Bacillus amyloliquefaciens; ≥480 KNU-B/g

Biochemical and Physiological Mechanisms

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units. Cofactor: Protein has several cofactor binding sites: Ca²⁺. Note: Binds 3 Ca²⁺ ions per subunit. Na⁺. Note: Binds 1 sod

Bioactivity

≥480 KNU-B/g

Accession #

P00692

CAS

9000-90-2

Enzyme Commission Number

EC 3.2.1.1

Molecule Type

Enzyme

Storage and Shipping

Concentration

from Bacillus amyloliquefaciens; ≥480 KNU-B/g

Storage

Store at 2-8°C,Argon charged

Shipped In

Wet ice

Unit definition

The activity of α-amylase is determined relative to a standard enzyme reference, and the enzyme activity unit is KNU(B)/g.