β-Mannosidase Activity Assay Kit (Micro Method)

Cat. No.: M1522080

GRADE & PURITY BioReagent

Overview

β-Mannosidase (EC 3.2.1.25, β-Man) belongs to the class of exo-hydrolases. It is also known as β-D-mannoside mannohydrolase or exo-β-D-mannanase. This enzyme catalyzes the cleavage of non-reducing terminal β-1,4-D-glycosidic bonds in manno-oligosaccharides, releasing a single mannose molecule. It is an essential hydrolase for the complete degradation of mannan. β-Mannosidase (β-Man) catalyzes the hydrolysis of p-nitrophenyl-β-D-mannopyranoside to produce p-nitrophenol (PNP), which has a characteristic absorption peak at 405 nm. The β-mannosidase activity can be calculated by measuring the increase rate of absorbance at 405 nm.

Specifications

Specifications & Purity

BioReagent

Grade

BioReagent

Application

Enzyme activity assay

Method Of Detection

Colorimetric Method

Detection instrument

Microplate reader

Detection wavelength

405nm

Storage and Shipping

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ for 6 months. Store in the dark.

Frequently Asked Questions

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.