CD3 Mouse mAb (AF594) - Primary antibody, specific to CD3

Cat. No.: Ab189662
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 5 μL/test
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG1
Clone Number
UCHT1
Conjugation
AF594
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10T
Ab189662-10T
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$42.90
25T
Ab189662-25T
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$82.90
100T
Ab189662-100T
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$251.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, 5 μL/test ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human CD3 Antibody, Monoclonal (UCHT1), could be used for Flow Cytometry

Specifications

Product Name
CD3 Mouse mAb (AF594) - Primary antibody, specific to CD3
Synonyms
CD3epsilon | IMD18 | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surface a
Specifications & Purity
ExactAb™, Validated, 5 μL/test
Biochemical and Physiological Mechanisms
The CD3 complex mediates signal transduction.
Host species
Mouse
Specificity
CD3
Immunogen
Human CD3ε
Positive Control
Flow Cytometry: Human peripheral blood lymphocytes
Conjugation
AF594
Excitation (nm)
Yellow-Green Laser(594nm)
Emission(nm)
618nm
Ex/Em(nm)
Ex:590nm;Em:618nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein G purified
Concentration
5 μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

CD3 Mouse mAb (AF594) (Ab189662) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with CD3 Mouse mAb (AF594) (Ab189662) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF594) (Ab190247).

CD3 Mouse mAb (AF594) (Ab189662) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with CD3 Mouse mAb (AF594) (Ab189662) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF594) (Ab190247). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ25F0927753Certificate of AnalysisJul 09, 2026 Ab189662
ZJ25F0927754Certificate of AnalysisJul 09, 2026 Ab189662
ZJ25F0927755Certificate of AnalysisJul 09, 2026 Ab189662
Solution Calculators
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