CD31 Rat mAb - Primary antibody, specific to CD31, Rat IgG2a

Cat. No.: Ab094526

GRADE & PURITY Carrier Free, Validated, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, IF/ICC

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

390

Conjugation

Unconjugated

Overview

Rat anti Mouse CD31 Antibody, Monoclonal (390), could be used for Flow, ICC/IF, Intravital imaging, in vivo CD31 blocking and so on.
Application
Flow: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
Intravital imaging: Use at an assay dependent concentration.
in vivo CD31 blocking: Use at an assay dependent concentration.
Protein Function
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Specifications

Product Name

CD31 Rat mAb - Primary antibody, specific to CD31, Rat IgG2a

Synonyms

Cd31 | Pecam | adhesion molecule | CD31 antigen | CD31 | CD31/EndoCAM | EndoCAM | FLJ34100 | FLJ58394 | GPIIA' | PECA1 | PECAM1 | PECAM-1 | PECAM-1, CD31/EndoCAM | platelet endothelial cell adhesion molecule | platelet endothelial cell adhesion molecule-1

Specifications & Purity

Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Rat

Specificity

CD31

Immunogen

C3H/HeJ mouse hematopoietic progenitor cell line 3

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, PBS Only, Validated

Isotype

Rat IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: CD31 Rat mAb (Ab094526)

CD31 Rat mAb (Ab094526) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD31 with CD31 Rat mAb (Ab094526) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD31 Rat mAb (Ab094526)

CD31 Rat mAb (Ab094526) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD31 (red) with CD31 Rat mAb (Ab094526) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• IF/ICC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• Intravital imaging:

  Use at an assay dependent concentration.;

• in vivo CD31 blocking:

  Use at an assay dependent concentration.

Associated Targets(Human)

PECAM1 Tbio Platelet endothelial cell adhesion molecule (0 Activities)

Discover Target: PECAM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)