GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Choline acetyltransferase (ChAT, EC 2.3.1.6) is the synthesizing enzyme of acetylcholine (Ach) and regulates Ach metabolism. Ach is the primary excitatory neurotransmitter that modulates esophageal smooth muscle motility.
ChAT activity is assayed using acetyl-CoA and choline as substrates. Under the action of ChAT, the reaction produces acetylcholine and coenzyme A. The product then reacts with a chromogenic agent, producing an absorption peak at 412 nm, from which ChAT activity is calculated.
Enzymatic reaction: acetyl-CoA + choline → CoA + O-acetylcholine.
Specifications
Specifications & Purity
BioReagent
Grade
BioReagent
Application
Enzyme activity assay
Method Of Detection
Colorimetric Method
Detection instrument
Microplate reader
Detection wavelength
412nm
Storage and Shipping
Storage
Store at 2-8°C,Protected from light
Shipped In
Wet ice
Stability And Storage
Store at 2-8℃ for 3 months. Store in the dark.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
