Diethyl pyrocarbonate (DEPC) - Moligand™,≥97% , CAS No.1609-47-8

CAS: 1609-47-8 Cat. No.: D137738 Formula: C₆H₁₀O₅ Molecular Weight: 162.14 Beilstein Registry Number: 637031 EC Number: 216-542-8

GRADE & PURITY Moligand™, ≥97%

Synonyms

DEP | 3,3',5,5'-tetraethylbenzidine | Diethyl ester of pyrocarbonic acid | diethyl pyrocarbonate | InChI=1/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H | Diethylpyrocarbonate | diethyl-pyrocarbonate | carbonic acid, ethoxycarbonyl ethyl ester | CAS-1609-4

Storage

Store at 2-8°C,Argon charged

Shipped In

Wet ice

Overview

Diethyl Pyrocarbonate has been shown to inhibit ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. This compound could be useful for specific inactivation of nucleases during isolation of undegraded polynucleotides. Diethyl Pyrocarbonate also inhibits PAF Acetylhydrolase (platelet-activating factor acetyl hydrolase), and has been used as a probe for the topography of 5.8S rRNA in yeast ribosomes.
An inhibitor of ryanodine binding at the ryanodine/Ca2+ channel

Specifications

Synonyms

DEP | 3,3',5,5'-tetraethylbenzidine | Diethyl ester of pyrocarbonic acid | diethyl pyrocarbonate | InChI=1/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H | Diethylpyrocarbonate | diethyl-pyrocarbonate | carbonic acid, ethoxycarbonyl ethyl ester | CAS-1609-4

Specifications & Purity

Moligand™, ≥97%

Biochemical and Physiological Mechanisms

Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.

Storage

Store at 2-8°C,Argon charged

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Purity

≥97%

Names and Identifiers

Pubchem Sid

488179737

Pubchem Sid Url

https://pubchem.ncbi.nlm.nih.gov/substance/488179737

Canonical Smiles

CCOC(=O)OC(=O)OCC

IUPAC Name

ethoxycarbonyl ethyl carbonate

InChIKey

FFYPMLJYZAEMQB-UHFFFAOYSA-N

INCHI

1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3

Isomeric SMILES

CCOC(=O)OC(=O)OCC

WGK Germany

3

RTECS

LQ9350000

Molecular Weight

162.14

Beilstein

637031

Reaxy-Rn

637031

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=637031&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Organic carbonic acids and derivatives

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Organic carbonic acids and derivatives
• Subclass: Not available
• Intermediate Tree Nodes: Not available
• Direct Parent: Organic carbonic acids and derivatives
• Alternative Parents: Organic oxides Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Aliphatic acyclic compounds
• Substituents: Carbonic acid derivative - Organic oxygen compound - Organic oxide - Hydrocarbon derivative - Organooxygen compound - Carbonyl group - Aliphatic acyclic compound
• Description: This compound belongs to the class of organic compounds known as organic carbonic acids and derivatives. These are compounds comprising the organic carbonic acid or a derivative thereof.
• External Descriptors: a small molecule

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Associated Targets(Human)

ALDH1A1 Tchem Aldehyde dehydrogenase 1A1 (77053 Activities)

Discover Target: ALDH1A1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SOAT2 Tchem Acyl coenzyme A:cholesterol acyltransferase 2 (288 Activities)

Discover Target: SOAT2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Associated Targets(non-human)

Soat1 Acyl coenzyme A:cholesterol acyltransferase 1 (295 Activities)

Discover Target: Soat1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Chemical and Physical Properties

• Solubility: Soluble in chloroform, ethanol (50% w/w), esters, ketones, and hydrocarbons.Slowly soluble in water with hydrolytic decomposition yielding ethanol and CO2., Soluble in chloroform, ethanol (50% w/w), esters, ketones, and hydrocarbons.Slowly soluble in wate
• Sensitivity: Heat and Moisture sensitive
• x_refractive_index: n20/D 1.398
• x_flash_point_drop_fahrenheit: 156.2 °F
• x_flash_point_drop_centigrade: 69℃
• x_boil_point_char: 93-94 °C
• Molecular Weight: 162.140 g/mol
• XLogP3: 1.400
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 6
• Exact Mass: 162.053 Da
• Monoisotopic Mass: 162.053 Da
• Topological Polar Surface Area: 61.800 Ų
• Heavy Atom Count: 11
• Formal Charge: 0
• Complexity: 126.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1