EB Solution (10 mg/mL) - BioReagent, Suitable for electrophoresis, 10 mg/ml in DMF , CAS No.1239-45-8

CAS: 1239-45-8 Cat. No.: E1526364 Formula: C₂₁H₂₀BrN₃ Molecular Weight: 394.32 EC Number: 214-984-6

GRADE & PURITY BioReagent, Suitable for electrophoresis, 10 mg/ml in DMF

Synonyms

10 mg/ml Ethidium Bromide Aqueous Solution

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

978

Overview

Ethidium Bromide (molecular formula: C₂₁H₂₀BrN₃, molecular weight: 394.31) is a highly sensitive fluorescent dye used for visualizing DNA in agarose and polyacrylamide gels. It emits an orange-red fluorescence signal when excited by a 302 nm UV transilluminator. EB is toxic; all EB-containing solutions must be decontaminated before disposal to prevent environmental pollution.  EB Solution (10 mg/mL) is prepared with sterile double-distilled water and is widely applied in electrophoresis of DNA and RNA samples.

Required Materials:

1. Electrophoresis running buffer (e.g., TAE, TBE)

2. Microwave oven

3. UV analyzer or gel documentation imaging system

Operating Protocol:

1. Melt 50 mL agarose gel solution (commonly 0.8-2% concentration) in a microwave oven.

2. Add 50–100 μL Ethidium Bromide stock solution (10 mg/mL) and mix gently to avoid air bubbles.

3. Pour the gel when cooled to 50–60°C (touchable without scalding). After complete solidification, remove the comb, submerge the gel in electrophoresis buffer, and load samples for electrophoresis.

4. Observe, photograph and document the results under a UV analyzer or gel imaging system.

Precautions:

1. Gel thickness should not exceed 0.5 cm; excessive thickness will reduce detection sensitivity.

2. Avoid prolonged UV exposure when recovering DNA fragments via gel electrophoresis.

3. EB is toxic and irritates the skin and eyes. Handle with extreme caution.

4. For personal safety, wear a lab coat, disposable gloves and a face mask during operation.

5. This product is for scientific research use by qualified professionals only. It must not be used for clinical diagnosis or treatment, food or pharmaceutical production, nor stored in residential premises.

Specifications

Synonyms

10 mg/ml Ethidium Bromide Aqueous Solution

Specifications & Purity

BioReagent, Suitable for electrophoresis, 10 mg/ml in DMF

Stability And Storage

Store at room temperature long term (12 months).Store in the dark.

Storage

Protected from light,Room temperature

Shipped In

Normal

Grade

BioReagent, Suitable for electrophoresis

Names and Identifiers

• Isomeric SMILES: CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-]
• WGK Germany: 3
• RTECS: SF7950000
• Molecular Weight: 394.32
• Reaxy-Rn: 3642536
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=3642536&ln=

Chemical and Physical Properties

• Sensitivity: Light-sensitive
• x_melt_point_char: 260-262°C

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Frequently Asked Questions

What does suitable for electrophoresis mean?

suitable for electrophoresis indicates that the product has been processed and tested for electrophoresis and blotting. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at room temperature, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1239-45-8, the molecular formula is C21H20BrN3, and the molecular weight is 394.32 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.