Endo F2, CAS No.37278-88-9

CAS: 37278-88-9 Cat. No.: E755020 EC Number: 998-699-9

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, His Tag, ≥90%(SDS-PAGE), ≥100 U/mL, Protein concentration: see COA

Accession #

A0AAQ1SX87

Protein Tag

C-His

Expression system

E. coli

Bioactivity

≥100U/ml

Overview

Endo F2 is a highly specific recombinant endoglycosidase derived from Elizabethkingia meningoseptica. It cleaves core-fucosylated biantennary complex-type and high-mannose oligosaccharides linked to asparagine residues from glycoproteins and glycopeptides. Endo F2 digests biantennary complex glycans approximately 20 times faster than high-mannose glycans. Its enzymatic activity is unaffected by the presence or absence of core fucosylation. Nevertheless, Endo F2 exhibits no activity against hybrid-type, triantennary or tetraantennary glycans. 

Components List

E755020	Component	400U	1KU	Storage
E755020A	Endo-β-N-acetylglucosaminidase F2	400U	1KU	-20℃. Avoid freeze/thaw cycle.
E755020B	10×GlycoBuffer 3 (500 mM Sodium acetate pH4.5)	1000μl	1000μl	-20℃. Avoid freeze/thaw cycle.

Operating Protocol

1. Before use, take out the Endo F2 reagent and centrifuge at 10,000 rpm for 10 seconds to collect all liquid at the bottom of the tube.

2. Mix 10 µg bovine fibrinogen with 1 µL of 500 mM Sodium Acetate (pH 4.5), add Endo F2, and top up with purified water to a total reaction volume of 20 µL. Mix gently, then incubate the reaction mixture at 37°C for 1 hour.

3. Analyze the digested products via SDS-PAGE or mass spectrometry.

Remark: Incubation duration and enzyme dosage should be optimized according to practical experimental conditions.

Precautions

Wear a lab coat and disposable nitrile gloves during operation to safeguard personal safety and health. This product is for research use only.

Specifications

Product Name

Endo F2, CAS No.37278-88-9

Synonyms

Endoglycosidase F2 | mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase | di-N-acetylchitobiosyl beta-N-acetylglucosaminidase | endo-beta-N-acetylglucosaminidase | endOF2

Grade

ActiBioPure™, Bioactive, High Performance, His Tag, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, His Tag, ≥90%(SDS-PAGE), ≥100 U/mL, Protein concentration: see COA

Bioactivity

≥100U/ml

Accession #

A0AAQ1SX87

SDS-PAGE

75 kDa

CAS

37278-88-9

Enzyme Commission Number

EC 3.2.1.96

Molecule Type

Enzyme

Product Line

biological_products

Storage and Shipping

Concentration

≥100 U/mL, Protein concentration: see COA

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (18 months). Avoid freeze/thaw cycle.

Unit definition

Quantity of enzyme sufficient to eliminate >95% carbohydrates from 10 μg denatured bovine fibrinogen in 10 μL reaction mix upon incubation at 37 °C for 1 h.