1.Dissolve 380g of acrylamide and 20g of N,N'-methylbisacrylamide in 600ml of H2O. Heating may be necessary to dissolve the acrylamide.
2.Adjust the volume to 1L with H2O.
3.Sterilize the solution by filtration (0.45 micron pore size).
4.Check the pH (should be 7.0 or less).
5.Store in dark bottles at room temperature.
Caution: Acrylamide is a neurotoxin. Use extreme caution when handling anything with acrylamide and bisacrylamide. Protective equipment should be worn.
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