Experiments to determine peak protein production
Experiments to determine peak protein production
Protein is the material basis of life, without which there is no life. It is therefore a substance that is closely associated with life and with all forms of vital activity.
Operation method
basic program
Materials and Instruments
Protein Move 1. 3×106 Sf9 cells were inoculated into 15 60 mm blood tissue culture dishes containing 5 ml of complete culture medium with 10% fetal bovine serum at 27°C for 2 h to allow the cells to adhere to the wall, and then each time, 7 dishes were infected with wild-type virus and 7 dishes with recombinant virus at an amount of the multiplicity of infections (MOI) of 10, and the remaining one dish was a non-infected control.2. Cells were harvested at different times after infection (from about 15 to 72 h after infection), and non-infected cells were harvested 15 h after infection. Cells and culture supernatants were transferred to centrifuge tubes and centrifuged at 1,000 g at 4°C for 10 min to harvest the cells.3. Process and analyze cells and supernatants (secreted recombinant protein). For more product details, please visit Aladdin Scientific website.
Fetal Bovine Serum
Culture Bottles Centrifuge