This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.
Operation method
Morphologic observation experiment on complex flat epithelium
Materials and Instruments
Microscope he Move 1. Staining: HE For more product details, please visit Aladdin Scientific website.
2. Visualization by the naked eye:
The specimen is a transverse section of the esophagus with an irregularly shaped lumen and a complex flat epithelium near the purplish-blue surface of the lumen.
3. low magnification observation:
What is observed in the transverse section of the esophagus is a vertical section of the complex flat epithelium. The complex squamous epithelium and some of the tissue below it are seen to form a protrusion into the lumen of the tube (which is actually a longitudinal fold in a three-dimensional structure). The complex flat epithelium consists of multiple layers of cells, each of which has a different shape. The junction between the epithelium and the deep connective tissue is undulating, separated by a basement membrane. The basement membrane is uniformly pink in color.
4. High magnification observation:
The morphology of the cells in each layer was observed from the basal surface of the epithelium to the luminal surface.
(1) Basal layer: located on the basement membrane, it is a layer of short columnar or cubic cells. The nucleus is deeply stained, ovoid, with little cytoplasm and unclear cell boundaries.
(2) Middle layer: located above the basal layer, composed of several layers of polygonal cells. The nuclei are larger and rounded.
(3) Surface layer: located on the superficial surface of the epithelium, composed of several layers of flat cells. The nuclei are small and pike-shaped.