Recombinant Human Adenosine Deaminase Protein FAQs

Recombinant Human Adenosine Deaminase Protein is a key enzyme in purine metabolism that catalyzes the irreversible deamination of adenosine to inosine and is widely used in immunology, biochemistry, and gene therapy research.

Product Name

Recombinant Human Adenosine Deaminase Protein

SKU

rp364764

Biological Function

Catalyzes the deamination of adenosine and 2-deoxyadenosine to inosine and 2-deoxyinosine, respectively

Relevance to Disease

ADA deficiency causes severe combined immunodeficiency (SCID); ADA is a target for enzyme replacement therapy

Expression System

Recombinant, expressed in a heterologous system (verify specific host in datasheet)

Recommended Storage

-20°C or -80°C; avoid repeated freeze-thaw cycles (verify on datasheet)

What Is Recombinant Human Adenosine Deaminase Protein?

Adenosine Deaminase (ADA; EC 3.5.4.4) is a ubiquitous enzyme of the purine salvage pathway that catalyzes the irreversible hydrolytic deamination of adenosine and 2-deoxyadenosine to inosine and 2-deoxyinosine, respectively. This reaction is essential for regulating intracellular and extracellular adenosine levels, which in turn modulates immune cell development and function.

Recombinant Human Adenosine Deaminase Protein (SKU: rp364764) is produced using recombinant DNA technology to yield a protein that mirrors the human native sequence. It is used as a research tool in enzymatic assays, immunological studies, and as a reference standard in biochemical workflows.

ADA deficiency is one of the most studied inborn errors of metabolism, accounting for approximately 10–15% of all cases of autosomal recessive severe combined immunodeficiency (SCID). This context makes recombinant ADA an important reagent in translational and basic immunology research.

How Should I Store and Reconstitute This Protein?

Proper storage and handling of Recombinant Human Adenosine Deaminase Protein are critical to preserving enzymatic activity and preventing degradation.

• Storage temperature: Store the lyophilized or liquid protein at -20°C for short-term use, or at -80°C for long-term storage. Always refer to the specific product datasheet (SKU: rp364764) for confirmed storage conditions.
• Avoid freeze-thaw cycles: Repeated freezing and thawing will reduce enzymatic activity. Prepare single-use aliquots immediately after the first reconstitution.
• Reconstitution: For lyophilized formats, reconstitute in the buffer recommended on the product datasheet (typically sterile PBS or manufacturer-specified buffer). Allow the vial to equilibrate to room temperature before opening. Gently pipette to dissolve—do not vortex.
• Carrier protein: Addition of a carrier protein such as 0.1% BSA may improve stability during storage of dilute solutions. Confirm compatibility with your assay before use.

If any visible particulate matter or turbidity is observed after reconstitution, the protein should not be used, as this may indicate aggregation or contamination.

What Are the Key Research Applications?

Recombinant Human Adenosine Deaminase Protein serves multiple roles in life-science research:

1. Enzymatic activity assays: ADA activity can be measured spectrophotometrically by monitoring the conversion of adenosine to inosine or by coupled assay systems. Recombinant ADA serves as both a positive control and a standard curve component in these assays.
2. Immunological research: Because ADA is critical for lymphocyte development, recombinant ADA is used to study T- and B-cell differentiation, proliferation, and signaling pathways affected by adenosine accumulation.
3. ADA deficiency disease models: Researchers use recombinant human ADA to rescue ADA-deficient cell lines or primary cells, providing mechanistic insights into ADA-SCID pathophysiology.
4. Drug discovery and inhibitor screening: ADA inhibitors (e.g., pentostatin, EHNA) are of pharmacological interest; recombinant ADA provides a reproducible enzyme source for high-throughput inhibitor screening.
5. RNA and nucleotide research: ADA is used to remove adenosine from reaction mixtures in specialized nucleotide metabolism studies and in vitro transcription quality-control workflows.

Always confirm that the recombinant protein's activity and purity specifications (available on the product datasheet) are appropriate for your specific experimental design.

Quality, Purity, and Lot-to-Lot Consistency

Research-grade recombinant proteins must meet defined quality standards to ensure reproducible experimental results. For Recombinant Human Adenosine Deaminase Protein (rp364764), researchers should review the Certificate of Analysis (CoA) provided with each lot, which typically includes:

• Purity: SDS-PAGE or HPLC-assessed purity, typically reported as ≥90% or higher for research-grade recombinant proteins (confirm exact specification on datasheet).
• Endotoxin level: Endotoxin content is particularly important for cell-based and in vivo assays. Check the CoA for LAL-tested endotoxin values.
• Biological activity: Specific activity (units/mg) confirmed by enzymatic assay, demonstrating that the protein is folded correctly and catalytically functional.
• Molecular weight confirmation: Western blot or mass spectrometry data confirming the expected molecular weight of the ADA protein.

If lot-to-lot variability is a concern in your assay, contact Aladdin Scientific technical support to request additional QC data or reserve sufficient stock from a single lot for your study duration.

Ordering, Shipping, and Technical Support

Recombinant Human Adenosine Deaminase Protein is available from Aladdin Scientific under SKU rp364764.

• Ordering: Place orders directly through the Aladdin Scientific website or contact your regional sales representative. Bulk quantities and custom formulations may be available upon request.
• Shipping: Recombinant proteins are typically shipped on dry ice to maintain cold-chain integrity. Confirm packaging details and shipping conditions with Aladdin Scientific at the time of order to ensure compliance with your institutional receiving requirements.
• Lead time: Standard lead times apply; contact Aladdin Scientific for current stock availability and estimated delivery timelines for your region.
• Technical support: For questions about experimental design, reconstitution troubleshooting, or activity optimization, contact Aladdin Scientific technical support with your lot number and CoA on hand for the most efficient assistance.
• Returns and refunds: Please refer to Aladdin Scientific's returns and refunds policy. Quality complaints accompanied by documented experimental data and the CoA will be reviewed by the technical team.

Frequently asked questions

What is the biological function of Human Adenosine Deaminase (ADA)?

ADA catalyzes the irreversible deamination of adenosine and 2-deoxyadenosine to inosine and 2-deoxyinosine, respectively, as part of the purine salvage pathway. This reaction is essential for regulating purine nucleoside levels and is critical for normal lymphocyte development and immune function. Deficiency of ADA leads to toxic accumulation of deoxyadenosine metabolites in lymphocytes, causing severe combined immunodeficiency (SCID).

How do I reconstitute Recombinant Human Adenosine Deaminase Protein (rp364764)?

Allow the vial to equilibrate to room temperature before opening. Reconstitute the lyophilized protein in the buffer specified on the product datasheet—typically sterile PBS or a manufacturer-recommended buffer—at the recommended concentration. Gently pipette to mix; do not vortex, as this may cause aggregation and loss of activity. Prepare single-use aliquots and store at -80°C to avoid repeated freeze-thaw cycles.

What assays can I use to verify ADA enzymatic activity?

ADA activity is commonly measured by a spectrophotometric assay monitoring the decrease in absorbance at 265 nm as adenosine is converted to inosine. Alternatively, coupled colorimetric or fluorometric assay kits are commercially available. When using Recombinant Human Adenosine Deaminase Protein (rp364764) as a positive control, compare the measured specific activity (units/mg) against the value stated in the Certificate of Analysis provided with the lot.

Is Recombinant Human Adenosine Deaminase Protein suitable for cell-based assays?

Suitability for cell-based assays depends on the endotoxin level and formulation of the specific lot. Always check the Certificate of Analysis for endotoxin content (typically tested by the LAL method) before use in cell culture experiments, as high endotoxin levels can confound cellular responses. Contact Aladdin Scientific technical support for guidance on endotoxin specifications for SKU rp364764.

What should I do if the reconstituted protein appears turbid or shows reduced activity?

Turbidity after reconstitution may indicate protein aggregation due to incorrect buffer conditions, temperature shock, or vortex mixing. If turbidity is observed, do not use the sample; contact Aladdin Scientific customer service with your lot number and CoA for troubleshooting or replacement evaluation. Reduced activity may also result from repeated freeze-thaw cycles, improper storage temperature, or use of incompatible buffers—review storage and handling conditions first.

References

1. Giblett ER, Anderson JE, Cohen F, Pollara B, Meuwissen HJ. Adenosine-deaminase deficiency in two patients with severely impaired cellular immunity. Lancet. 1972;2(7786):1067-1069.
2. Hershfield MS. Adenosine deaminase deficiency: clinical expression, molecular basis, and therapy. Seminars in Hematology. 1998;35(4):291-298.
3. Aladdin Scientific. Recombinant Human Adenosine Deaminase Protein, SKU rp364764. Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.