Recombinant Human CFAP58 Protein: Handling & Usage Protocol

Recombinant Human CFAP58 Protein is a research-grade reagent designed to support investigations into cilia-associated biological processes, requiring careful reconstitution, precise storage, and validated handling to deliver reproducible experimental results.

Product Name

Recombinant Human CFAP58 Protein

SKU

rp375998

Protein Origin

Human (Homo sapiens)

Protein Type

Recombinant, cilia and flagella associated protein 58

Recommended Storage

-20°C or -80°C; avoid repeated freeze-thaw cycles

Primary Research Area

Cilia biology, cell motility, ciliopathy research

Overview of CFAP58 and Research Significance

CFAP58 (Cilia and Flagella Associated Protein 58) is a protein encoded by the CFAP58 gene in humans. It is associated with the structural and functional integrity of cilia and flagella, organelles critical for cellular motility, fluid movement, and signal transduction. Dysregulation or mutation of CFAP58 has been implicated in ciliopathies — a class of disorders arising from defective cilia function, which can affect organs including the lungs, kidneys, and reproductive system.

Recombinant Human CFAP58 Protein (rp375998) from Aladdin Scientific enables researchers to study CFAP58 function, screen for binding partners, develop antibodies, and investigate its role in disease models. This reagent is produced recombinantly to ensure batch-to-batch consistency and defined purity suitable for in vitro research applications.

Reconstitution Protocol

Proper reconstitution is essential to maintain protein activity and prevent aggregation. Follow these steps when reconstituting Recombinant Human CFAP58 Protein:

1. Equilibrate: Allow the vial to equilibrate to room temperature for 10–15 minutes before opening to prevent condensation from contacting the lyophilized material.
2. Centrifuge: Briefly centrifuge the sealed vial (1,000–2,000 × g, 30 seconds) to collect lyophilized material at the bottom of the vial.
3. Add reconstitution buffer: Reconstitute the protein in sterile, ultrapure water or the recommended buffer (e.g., PBS, pH 7.4) to yield the desired working concentration. Add the reconstitution volume slowly along the side of the vial; do not add directly onto the lyophilized cake.
4. Dissolve gently: Allow the vial to stand for 5 minutes, then gently swirl or pipette to mix. Do NOT vortex, as this can denature the protein.
5. Verify solubility: Inspect visually for a clear, particulate-free solution. If cloudiness persists, centrifuge at low speed and use the supernatant.

Note: Actual reconstitution volume and recommended buffer should be confirmed from the product-specific datasheet. Refer to the Certificate of Analysis (CoA) supplied with each lot.

Storage and Stability Guidelines

To preserve the biological activity and structural integrity of Recombinant Human CFAP58 Protein, adhere to the following storage guidelines:

• Short-term storage (up to 1 week): Store at 4°C in a suitable carrier protein-containing buffer (e.g., 0.1–0.5% BSA in PBS) to stabilize the protein during frequent-use periods.
• Long-term storage: Aliquot the reconstituted protein into single-use volumes and store at -20°C or preferably -80°C to maximize shelf life.
• Avoid freeze-thaw cycles: Repeated freezing and thawing significantly reduces protein activity. Prepare working aliquots prior to first use.
• Carrier protein addition: Where compatibility with downstream assays permits, adding a carrier protein (e.g., BSA) can improve stability and reduce non-specific adsorption to tube surfaces.
• Expiry: Refer to the lot-specific CoA for the recommended use-by date. Lyophilized protein is typically stable for up to 12 months at -20°C when stored desiccated; confirm with product datasheet.

Flag: Specific shelf-life data and stability conditions for rp375998 should be verified against the official product datasheet and CoA.

Quality Verification and Functional Application

Before proceeding with critical experiments, verify the quality and activity of the reconstituted Recombinant Human CFAP58 Protein using the following approaches:

Quality Control Checks

• SDS-PAGE: Run a reducing SDS-PAGE gel to confirm the expected molecular weight band and assess purity. Compare against the molecular weight stated in the product datasheet.
• Western Blot: Confirm identity using an anti-CFAP58 antibody under reducing conditions.
• Protein quantification: Use a BCA or Bradford assay to determine the actual protein concentration of the reconstituted solution; cross-check against the stated concentration in the CoA.

Research Applications

Recombinant Human CFAP58 Protein is suitable for use in a range of in vitro research applications, including but not limited to:

• Biochemical interaction studies (co-immunoprecipitation, pull-down assays)
• Antibody production and immunogen preparation
• ELISA standard curve development
• Structural and biophysical characterization studies
• Cell-free functional assays investigating cilia-associated protein networks

Note: Suitability for specific applications should be confirmed by the researcher. Refer to literature on CFAP58 biology for validated assay conditions.

Safety, Disposal, and Ordering Information

Safety: Recombinant Human CFAP58 Protein is intended for in vitro research use only. It is not approved for use in humans or animals. Handle all recombinant proteins using standard laboratory biosafety practices (BSL-1 or as designated by your institution). Wear appropriate PPE including gloves, lab coat, and eye protection. Consult your institution's biosafety guidelines for the handling and disposal of recombinant protein products.

Disposal: Dispose of protein-containing materials in accordance with local, national, and institutional regulations governing biological waste. Inactivate materials by autoclaving or chemical treatment as required before disposal.

Ordering: Recombinant Human CFAP58 Protein is available from Aladdin Scientific under SKU rp375998. Contact your regional Aladdin Scientific representative or visit the Aladdin Scientific website to place an order, request a quotation, or obtain the latest product datasheet and Certificate of Analysis.

Frequently asked questions

What is the recommended reconstitution buffer for Recombinant Human CFAP58 Protein?

The protein is generally reconstituted in sterile, ultrapure water or PBS (pH 7.4). For optimal stability, a carrier protein such as 0.1% BSA may be added. Always consult the lot-specific Certificate of Analysis (CoA) and product datasheet supplied with SKU rp375998 for the manufacturer's specific recommendation.

How should I store Recombinant Human CFAP58 Protein to maintain its activity?

For short-term use (up to one week), store the reconstituted protein at 4°C in a stabilizing buffer. For long-term storage, aliquot into single-use volumes and store at -20°C or -80°C. Avoid repeated freeze-thaw cycles, as these significantly reduce protein bioactivity.

What applications is Recombinant Human CFAP58 Protein validated for?

Recombinant Human CFAP58 Protein (rp375998) is suitable for in vitro research applications including biochemical interaction studies (pull-down, co-IP), antibody development, ELISA standard preparation, and biophysical characterization. Researchers should validate suitability for each specific assay in their own laboratory context.

Can Recombinant Human CFAP58 Protein be used in cell-based assays?

Recombinant Human CFAP58 Protein is primarily intended for in vitro biochemical assays. Use in cell-based applications may be possible but requires independent validation by the researcher, including assessment of cell uptake, potential immunogenicity, and functional readout. The product is not intended for in vivo or clinical use.

How do I confirm the identity and purity of the reconstituted protein?

Run a reducing SDS-PAGE to verify the expected molecular weight band and assess purity by comparing band intensity. Confirm identity by Western blot using a specific anti-CFAP58 antibody. Protein concentration should be measured using a BCA or Bradford assay and compared to the CoA value.

References

1. Aladdin Scientific. Recombinant Human CFAP58 Protein, SKU rp375998. Product Datasheet and Certificate of Analysis.
2. Reiter JF, Leroux MR. Genes and molecular pathways underpinning ciliopathies. Nature Reviews Molecular Cell Biology. 2017;18(9):533-547. doi:10.1038/nrm.2017.60
3. Fliegauf M, Benzing T, Omran H. When cilia go wrong: cilia defects and ciliopathies. Nature Reviews Molecular Cell Biology. 2007;8(11):880-893. doi:10.1038/nrm2278

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.