Recombinant Human CHMP7 Protein: Function & Research Applications

Recombinant Human CHMP7 Protein is a key member of the ESCRT-III (Endosomal Sorting Complexes Required for Transport) pathway, playing essential roles in nuclear envelope reformation, membrane abscission, and multivesicular body biogenesis.

Protein Name

Charged Multivesicular Body Protein 7 (CHMP7)

Gene Name

CHMP7

Protein Family

ESCRT-III / Snf7 superfamily

Key Biological Roles

Nuclear envelope sealing, membrane remodeling, multivesicular body formation, viral budding

Expression System

Recombinant production (specific host system subject to review)

Research Applications

Biochemical assays, protein interaction studies, functional cellular assays, drug discovery

SKU

rp374242

What Is CHMP7? Biology and Protein Overview

CHMP7 (Charged Multivesicular Body Protein 7) is a member of the ESCRT-III (Endosomal Sorting Complexes Required for Transport III) family of proteins. The ESCRT pathway is evolutionarily conserved and orchestrates a diverse range of cellular membrane-remodeling events, including the formation of intraluminal vesicles within multivesicular bodies (MVBs), cytokinetic abscission, nuclear envelope (NE) reformation following mitosis, and the budding of enveloped viruses.

Unlike many classical ESCRT-III subunits, CHMP7 contains a distinctive N-terminal ESCRT-II–like domain fused to a C-terminal ESCRT-III–like coiled-coil region. This hybrid architecture allows CHMP7 to serve as a critical adaptor that bridges the ESCRT-II and ESCRT-III subcomplexes, recruiting downstream effectors such as VPS4 ATPase for membrane scission. CHMP7 is ubiquitously expressed in human tissues, reflecting its fundamental importance in membrane homeostasis.

Key Functions of CHMP7 in Cellular Processes

Nuclear Envelope Sealing: One of the most intensively studied roles of CHMP7 is its involvement in post-mitotic nuclear envelope reformation. At the end of mitosis, CHMP7 is recruited to chromatin surfaces where it nucleates ESCRT-III filament assembly. This process seals the reforming nuclear envelope, preventing cytoplasmic contents from mixing with nuclear material and ensuring genome integrity. Dysregulation of this process has been linked to nuclear envelope rupture events observed in cancer cells and laminopathies.

Multivesicular Body (MVB) Biogenesis: CHMP7 contributes to the canonical ESCRT pathway governing the sorting of ubiquitinated membrane cargo into intraluminal vesicles of late endosomes (MVBs). Efficient MVB formation is critical for receptor downregulation, lysosomal degradation, and exosome secretion.

Viral Budding: Several enveloped viruses, including HIV-1, exploit the ESCRT machinery for efficient budding from the plasma membrane. CHMP7, as a component of this system, represents a potential target for antiviral intervention strategies.

Membrane Abscission in Cytokinesis: During the final step of cell division, ESCRT-III—including CHMP7—mediates abscission of the intercellular bridge, ensuring accurate segregation of genetic material into daughter cells.

Research Applications of Recombinant Human CHMP7 Protein

Recombinant Human CHMP7 Protein (SKU: rp374242) from Aladdin Scientific is designed to support a broad spectrum of in vitro and cell-based research applications:

• Protein–Protein Interaction Studies: CHMP7's dual ESCRT-II/ESCRT-III architecture makes it an important tool for mapping interactions with upstream ESCRT-II subunits (e.g., EAP20/VPS25) and downstream ESCRT-III subunits (e.g., CHMP2A, CHMP4B), as well as the AAA-ATPase VPS4.
• Biochemical and Biophysical Assays: Suitable for use in ELISA, Western blot validation, surface plasmon resonance (SPR), co-immunoprecipitation, and pull-down assays to characterize binding partners and inhibitors.
• Structural Biology: Recombinant CHMP7 can be employed in cryo-electron microscopy (cryo-EM) and X-ray crystallography studies aimed at resolving the structural basis of ESCRT-III filament nucleation.
• Drug Discovery: As a node in the ESCRT-III pathway relevant to cancer, neurodegeneration, and viral infection, CHMP7 is an emerging target for small-molecule and biologics screening campaigns.
• Functional Cell Biology: When combined with cell-free reconstitution systems or microinjection approaches, recombinant CHMP7 enables mechanistic dissection of nuclear envelope sealing and membrane scission events.

Handling, Storage, and Quality Considerations

To maintain the functional integrity and biological activity of Recombinant Human CHMP7 Protein, researchers should observe the following best practices:

• Storage: Store lyophilized or liquid protein preparations according to the certificate of analysis (CoA) provided with each lot. Typical recommendations for recombinant proteins include storage at −20 °C or −80 °C; avoid repeated freeze–thaw cycles.
• Reconstitution: Reconstitute lyophilized preparations using the buffer specified in the product datasheet. Gently mix by pipetting; do not vortex, as vigorous agitation may cause aggregation or denaturation.
• Quality Control: Each lot of Aladdin recombinant protein is subject to purity analysis (typically by SDS-PAGE) and identity confirmation. Specific purity values and endotoxin levels are detailed in the accompanying CoA.
• Working Concentrations: Optimal working concentrations will vary by assay type and must be determined empirically by the end user for each experimental system.

Note: Specific purity, formulation, and storage details for rp374242 should be confirmed against the current product datasheet and CoA available on the Aladdin Scientific website.

Why Choose Aladdin Scientific Recombinant Human CHMP7 Protein?

Aladdin Scientific is committed to supplying high-quality recombinant proteins that meet the rigorous demands of modern life science research. Recombinant Human CHMP7 Protein (rp374242) offers researchers:

• Reliable lot-to-lot consistency supported by stringent QC testing
• Full traceability with accompanying certificate of analysis
• Responsive technical support from scientists with expertise in recombinant protein biology
• Competitive pricing for both individual researchers and institutional procurement teams

For ordering information, bulk inquiries, or technical questions regarding rp374242, please contact Aladdin Scientific's customer service team or visit the product page directly.

Frequently asked questions

What is the biological function of CHMP7 in human cells?

CHMP7 is an ESCRT-III family member that functions as a molecular bridge between the ESCRT-II and ESCRT-III subcomplexes. Its primary roles include post-mitotic nuclear envelope sealing, multivesicular body biogenesis, cytokinetic abscission, and supporting the budding of enveloped viruses. These functions collectively make CHMP7 a central regulator of cellular membrane remodeling.

What research applications is Recombinant Human CHMP7 Protein suitable for?

Recombinant Human CHMP7 Protein (rp374242) is suitable for a range of applications including protein–protein interaction studies (pull-down, co-IP, SPR), biochemical assays (ELISA, Western blot), structural biology (cryo-EM, crystallography), drug discovery screening, and cell-free functional reconstitution assays. Researchers should validate optimal conditions for each specific assay.

How should Recombinant Human CHMP7 Protein be stored to maintain activity?

Recombinant proteins should generally be stored at −20 °C or −80 °C and protected from repeated freeze–thaw cycles, which can reduce biological activity and lead to aggregation. Please refer to the certificate of analysis (CoA) and product datasheet for lot-specific storage conditions, recommended buffers, and shelf-life information for rp374242.

Is CHMP7 relevant to disease research?

Yes. CHMP7 has been implicated in several disease contexts. Aberrant nuclear envelope rupture—partly involving dysregulated ESCRT-III/CHMP7 activity—has been observed in cancer cells and in cells from patients with laminopathies (diseases caused by mutations in nuclear lamina proteins). Additionally, the ESCRT machinery exploited by viruses such as HIV-1 makes CHMP7 a subject of interest in antiviral research.

What distinguishes CHMP7 structurally from other ESCRT-III family members?

Unlike typical ESCRT-III subunits, which contain only an ESCRT-III/Snf7-like coiled-coil domain, CHMP7 has a unique hybrid architecture featuring an N-terminal ESCRT-II–like domain fused to a C-terminal ESCRT-III–like region. This structural duality enables CHMP7 to act as an adaptor bridging ESCRT-II and ESCRT-III complexes, a function not shared by most other CHMP family members.

References

1. Vietri M, Radulovic M, Stenmark H. The many functions of ESCRTs. Nature Reviews Molecular Cell Biology. 2020;21(1):25-42. doi:10.1038/s41580-019-0177-4
2. Olmos Y, Hodgson L, Bhatt J, Bhatt J, Carlton JG. ESCRT-III controls nuclear envelope reformation. Nature. 2015;522(7555):236-239. doi:10.1038/nature14503
3. Gu M, LaJoie D, Chen OS, et al. LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells. PNAS. 2017;114(11):E2166-E2175. doi:10.1073/pnas.1613916114
4. Henne WM, Buchkovich NJ, Emr SD. The ESCRT pathway. Developmental Cell. 2011;21(1):77-91. doi:10.1016/j.devcel.2011.05.015

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.