Recombinant Human CHRAC1 Protein: FAQs & Specs

Recombinant Human CHRAC1 Protein is a recombinant form of the human chromatin accessibility complex subunit 1, produced for use in epigenetics, chromatin remodeling, and protein–protein interaction research.

Product Name

Recombinant Human CHRAC1 Protein

SKU

rp369384

Protein Function

Subunit of the CHRAC chromatin remodeling complex; involved in regulating chromatin accessibility and DNA replication

Expression System

Recombinant (specific host system to be confirmed; see review flags)

Recommended Storage

Store at -80 °C; avoid repeated freeze-thaw cycles (confirm with datasheet)

Primary Research Applications

Chromatin remodeling assays, protein interaction studies, epigenetics research

What Is CHRAC1 and Why Is It Important?

CHRAC1 (Chromatin Accessibility Complex Subunit 1) is a component of the CHRAC complex, an ATP-dependent chromatin remodeling assembly related to the ISWI family of complexes. The CHRAC complex, which includes CHRAC1 along with other subunits such as POLE3, ACF1, and SNF2H, facilitates nucleosome sliding and increases chromatin accessibility. These activities are critical for processes including DNA replication, transcription regulation, and DNA damage repair. Recombinant Human CHRAC1 Protein allows researchers to study the biochemical role of this subunit in vitro, including its contributions to complex assembly and histone-binding activity.

Product Specifications and Quality

Recombinant Human CHRAC1 Protein (SKU: rp369384) is produced using a recombinant expression system. Researchers should consult the official product datasheet for confirmed details on purity grade, molecular weight (the full-length human CHRAC1 protein is approximately 15 kDa based on gene-sequence-derived estimates), expression host, endotoxin levels, and formulation buffer. Quality control testing typically includes SDS-PAGE and/or Western blot analysis to confirm identity and purity. Precise lot-specific data, including purity percentage and activity validation, are provided with each shipment.

Note: Specific numerical values for purity and endotoxin have not been confirmed in the source material and are flagged for review.

Storage, Reconstitution, and Handling

Proper handling of Recombinant Human CHRAC1 Protein is essential to maintain activity and integrity. General best practices for recombinant proteins of this class include:

• Storage: Store at -80 °C for long-term stability. Short-term storage at -20 °C may be acceptable for periods up to one month, but confirm with the product datasheet.
• Freeze-Thaw Cycles: Minimize repeated freeze-thaw cycles by preparing single-use aliquots upon first thaw.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer specified on the datasheet. Centrifuge the vial briefly before opening. Allow the protein to dissolve gently without vortexing.
• Carrier Protein: Adding BSA (0.1–1%) to the working solution may help stabilize dilute preparations.

Always refer to the official Aladdin Scientific product datasheet for lot-specific reconstitution volumes and storage recommendations.

Research Applications for Recombinant Human CHRAC1 Protein

Recombinant Human CHRAC1 Protein is suitable for a range of in vitro research applications, including:

• Chromatin Remodeling Assays: Used as a component or control in nucleosome-remodeling assay systems to assess CHRAC complex activity.
• Protein–Protein Interaction Studies: Suitable for co-immunoprecipitation (Co-IP), pull-down assays, and surface plasmon resonance (SPR) experiments to map interactions with CHRAC complex partners such as POLE3, ACF1, and SNF2H.
• Structural Biology: Can be incorporated into complex reconstitution experiments for X-ray crystallography or cryo-EM studies.
• Antibody Development and Validation: Useful as an antigen standard for generating or validating CHRAC1-specific antibodies.
• Epigenetics Research: Supports mechanistic studies on chromatin accessibility regulation relevant to gene expression and DNA replication.

Ordering and Support

Recombinant Human CHRAC1 Protein is available from Aladdin Scientific under SKU rp369384. To place an order, contact your regional Aladdin Scientific representative or visit the product page on the Aladdin Scientific website. For technical questions regarding experimental design, reconstitution troubleshooting, or compatibility with specific assay systems, contact Aladdin Scientific technical support. Bulk quantities and custom formulations may be available upon request; please inquire with the sales team for pricing and lead times.

Frequently asked questions

What is CHRAC1 and what role does it play in the cell?

CHRAC1 is a subunit of the chromatin accessibility complex (CHRAC), an ATP-dependent chromatin remodeling complex belonging to the ISWI family. It works together with subunits such as POLE3, ACF1, and SNF2H to facilitate nucleosome sliding, thereby increasing chromatin accessibility for processes such as DNA replication and transcription.

What expression system is used to produce Recombinant Human CHRAC1 Protein (rp369384)?

The specific expression host system for rp369384 is not detailed in the available source material. Researchers should consult the official Aladdin Scientific product datasheet or contact technical support for confirmed information on the expression system, tag configuration, and purification method.

How should I store and handle Recombinant Human CHRAC1 Protein to maintain its activity?

For long-term storage, keep the protein at -80 °C and avoid repeated freeze-thaw cycles by preparing single-use aliquots upon first thaw. If the protein is supplied in lyophilized form, reconstitute it gently in the recommended buffer without vortexing. Always refer to the lot-specific datasheet provided with your product for precise storage and reconstitution instructions.

What research applications is Recombinant Human CHRAC1 Protein suitable for?

Recombinant Human CHRAC1 Protein is suitable for chromatin remodeling assays, protein–protein interaction studies (Co-IP, pull-down, SPR), structural biology complex reconstitution, antibody development and validation, and mechanistic epigenetics research. It is intended for research use only and is not approved for diagnostic or therapeutic applications.

How do I order Recombinant Human CHRAC1 Protein from Aladdin Scientific?

You can order Recombinant Human CHRAC1 Protein using SKU rp369384 through the Aladdin Scientific website or by contacting your regional sales representative. For bulk quantities, custom formulations, or pricing inquiries, please reach out to the Aladdin Scientific sales team directly.

References

1. Ito T, Bulger M, Pazin MJ, Kobayashi R, Kadonaga JT. ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor. Cell. 1997;90(1):145-155. doi:10.1016/S0092-8674(00)80321-9
2. Varga-Weisz PD, Wilm M, Bonte E, Dumas K, Mann M, Becker PB. Chromatin-remodelling factor CHRAC contains the ATPases ISWI and topoisomerase II. Nature. 1997;388(6642):598-602. doi:10.1038/41587
3. Aladdin Scientific. Recombinant Human CHRAC1 Protein, SKU rp369384 — Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.