Recombinant Human GIGYF1 Protein: Research Guide & Applications

Recombinant Human GIGYF1 Protein is a recombinant form of the GRB10-interacting GYF protein 1, produced for use as a research reagent in studies of mRNA translation regulation, insulin receptor signaling, and GYF domain-mediated protein–protein interactions.

Product Name

Recombinant Human GIGYF1 Protein

SKU

rp372896

Gene Name

GIGYF1 (GRB10-Interacting GYF Protein 1)

Species Origin

Human (Homo sapiens)

Protein Form

Recombinant

Research Area

Translation regulation, insulin signaling, RNA biology

Biological Background of GIGYF1

GIGYF1 (GRB10-Interacting GYF Protein 1) is a member of the GYF domain-containing protein family. It was originally identified through its interaction with GRB10, an adaptor protein involved in insulin and insulin-like growth factor (IGF) receptor signaling pathways. The GYF domain is a conserved proline-rich sequence-binding module that mediates specific protein–protein interactions critical for signal transduction and post-transcriptional regulation.

GIGYF1, together with its paralog GIGYF2, has been shown to participate in the regulation of mRNA translation and decay. These proteins associate with the 4EHP (eIF4E2) cap-binding protein and components of the CCR4–NOT deadenylase complex, thereby enabling translational repression of specific target mRNAs. This mechanism is relevant in developmental contexts, cellular stress responses, and the regulation of gene expression networks downstream of receptor tyrosine kinase activation.

Disruption of GIGYF1 function has been linked to altered insulin signaling and is under investigation for potential roles in metabolic disease and neurodevelopmental disorders. As a result, Recombinant Human GIGYF1 Protein serves as a valuable tool for dissecting these molecular pathways in vitro.

Protein Properties and Specifications

Recombinant Human GIGYF1 Protein (SKU: rp372896) is produced recombinantly for research use. Key biochemical characteristics of GIGYF1 relevant to experimental design include its GYF domain, which binds proline-rich motifs (typically PPPPGHR or similar sequences), and its extended intrinsically disordered regions that facilitate interactions with multiple binding partners including 4EHP and trinucleotide repeat-containing proteins.

Researchers should consult the product-specific datasheet for confirmed details on expression host system, tag configuration, purity grade (typically verified by SDS-PAGE and/or HPLC), endotoxin level, and formulation buffer. These parameters directly impact downstream assay performance, including binding assays, enzymatic studies, and cell-based functional experiments.

Note: Specific numerical values for molecular weight, purity percentage, endotoxin limits, and concentration are not reproduced here to avoid inaccuracy; please refer to the official Aladdin Scientific product datasheet for rp372896 for all confirmed specifications.

Reconstitution, Handling, and Storage Guidelines

Reconstitution: If supplied in lyophilized form, centrifuge the vial briefly before opening to collect all material at the bottom. Reconstitute the protein in sterile, ultrapure water or the buffer specified on the product datasheet. Allow the solution to equilibrate for several minutes at room temperature with gentle swirling; avoid vigorous vortexing, which may denature the protein. The recommended reconstitution volume and resulting concentration are stated on the product-specific datasheet.

Handling: Work under aseptic conditions to prevent microbial contamination. Use protein low-binding tubes and pipette tips to minimize adsorptive losses. If the protein is intended for cell-based assays, ensure all buffers and additives are sterile and cell-culture grade.

Storage: Upon receipt, store the lyophilized protein at –20 °C or –80 °C as directed on the datasheet. Reconstituted protein should be aliquoted to avoid repeated freeze–thaw cycles, which can lead to aggregation and loss of activity. Working aliquots may be stored at 4 °C for short-term use (typically up to one week); long-term storage should be at –80 °C with a carrier protein (e.g., 0.1% BSA) if recommended. Always refer to the product datasheet for product-specific storage conditions.

Research Applications

Recombinant Human GIGYF1 Protein is suitable for a range of in vitro research applications, including but not limited to:

• Protein–Protein Interaction Studies: GIGYF1's GYF domain and disordered regions make it an ideal target for pull-down assays, co-immunoprecipitation validation, surface plasmon resonance (SPR), and isothermal titration calorimetry (ITC) to characterize interactions with GRB10, 4EHP, trinucleotide repeat proteins, and CCR4–NOT complex subunits.
• Translation Repression Assays: The protein can be used in cell-free translation systems to study the mechanism by which the GIGYF1–4EHP complex suppresses cap-dependent translation of target mRNAs.
• Insulin and IGF Signaling Research: As a downstream effector of GRB10, GIGYF1 can be employed in reconstitution experiments to dissect receptor tyrosine kinase signaling cascades.
• Antibody Validation: Recombinant GIGYF1 serves as a positive control antigen for validating GIGYF1-specific antibodies by Western blot, ELISA, or dot blot.
• Structural Biology: The recombinant protein may support crystallographic or cryo-EM studies aimed at resolving GIGYF1 domain architecture and interaction interfaces.

Researchers should optimize protein concentrations, buffer conditions, and assay formats empirically for each specific application.

Quality Assurance and Regulatory Information

Recombinant Human GIGYF1 Protein (rp372896) supplied by Aladdin Scientific is manufactured for research use only (RUO). It is not approved or validated for diagnostic, therapeutic, or veterinary use.

Each lot is subject to quality control testing as detailed in the accompanying Certificate of Analysis (CoA). Typical QC assessments for recombinant proteins include purity assessment by SDS-PAGE under reducing and/or non-reducing conditions, identity confirmation by Western blot or mass spectrometry, and endotoxin determination by LAL assay. Specific QC data are lot-dependent and available upon request.

For any questions regarding product specifications, lot-specific CoA, custom formulations, or bulk ordering, please contact Aladdin Scientific's technical support team.

Frequently asked questions

What is GIGYF1 and why is it important in research?

GIGYF1 (GRB10-Interacting GYF Protein 1) is a GYF domain-containing adaptor protein involved in mRNA translation repression and insulin/IGF receptor signaling. It is important because it links receptor tyrosine kinase activity to post-transcriptional gene regulation via the 4EHP–CCR4–NOT axis, making it relevant to metabolic disease, neurodevelopmental biology, and RNA regulation research.

How should I reconstitute and store Recombinant Human GIGYF1 Protein?

If supplied lyophilized, briefly centrifuge the vial before opening, then reconstitute in sterile ultrapure water or the buffer specified on the datasheet. Aliquot the reconstituted protein to avoid freeze–thaw cycles and store long-term at –80 °C. Always consult the product-specific datasheet (rp372896) for exact reconstitution volumes and storage conditions.

What applications is Recombinant Human GIGYF1 Protein validated for?

Recombinant Human GIGYF1 Protein is suitable for protein–protein interaction studies (pull-down, SPR, ITC), cell-free translation repression assays, antibody validation (Western blot, ELISA), and structural biology studies. Researchers should empirically validate performance in each specific assay format.

Is Recombinant Human GIGYF1 Protein suitable for clinical or diagnostic use?

No. Recombinant Human GIGYF1 Protein (rp372896) from Aladdin Scientific is labeled for research use only (RUO) and has not been validated or approved for diagnostic, therapeutic, or veterinary applications.

How does GIGYF1 interact with GRB10 and what is the functional significance?

GIGYF1 interacts with GRB10 via its GYF domain, which recognizes proline-rich motifs present in GRB10. GRB10 itself is a negative regulator of insulin and IGF-1 receptor signaling, and the GIGYF1–GRB10 interaction is thought to couple receptor-level signaling events to downstream translational control mechanisms, though the precise functional consequences continue to be investigated.

References

1. Morita M, et al. A novel 4EHP-GIGYF2 translational repressor complex is essential for mammalian development. Molecular and Cellular Biology. 2012.
2. Giovannone B, et al. GIGYF1 and GIGYF2 proteins associate with mRNA decapping complexes. Biochemical and Biophysical Research Communications. 2009.
3. Iossifov I, et al. The contribution of de novo coding mutations to autism spectrum disorder. Nature. 2014.
4. Aladdin Scientific. Recombinant Human GIGYF1 Protein, SKU rp372896. Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.