Recombinant Human GIMAP8 Protein: Research Guide

Recombinant Human GIMAP8 Protein is a recombinant form of the GTPase IMAP family member 8, a key regulator of lymphocyte survival and immune homeostasis, produced for use in biochemical, cell biology, and immunological research applications.

Product Name

Recombinant Human GIMAP8 Protein

SKU

rp372902

Protein Family

GTPase IMAP (GIMAP) family

Species Origin

Human (Homo sapiens)

Primary Research Area

Immunology, T cell biology, lymphocyte survival

Format

Recombinant protein; refer to product datasheet for storage and formulation details

What Is GIMAP8? Biology and Significance

GIMAP8 (GTPase IMAP Family Member 8) belongs to the GIMAP (GTPase of the immunity-associated protein) family, a group of small GTPases predominantly expressed in immune cells, particularly T lymphocytes. The GIMAP family has been implicated in the regulation of lymphocyte development, survival, and apoptosis, making these proteins central to understanding immune homeostasis.

GIMAP8 is encoded by the GIMAP8 gene located within the GIMAP gene cluster. Like other family members, it contains a conserved AIG1 (ATPase or GTPase) domain that is characteristic of the GIMAP superfamily. Studies suggest that GIMAP proteins, including GIMAP8, interact with one another and with components of the apoptotic machinery, potentially modulating T cell survival signals.

Dysregulation of GIMAP family members has been associated with immune dysfunction, autoimmune predisposition, and altered lymphocyte homeostasis in various model organisms. Research into GIMAP8 therefore has implications for understanding T cell-mediated immunity and may inform studies of immune-related diseases.

Product Description and Quality Attributes

Aladdin Scientific's Recombinant Human GIMAP8 Protein (SKU: rp372902) is produced using recombinant expression technology to deliver a high-quality research-grade protein. This recombinant protein is intended for use in a range of in vitro biochemical and cell-based research applications.

Key quality considerations for recombinant proteins of this class typically include:

• Purity: Recombinant proteins are generally evaluated by SDS-PAGE and/or HPLC to confirm purity levels suitable for research use. Researchers should consult the product-specific Certificate of Analysis (CoA) for confirmed purity data.
• Biological Activity: Functional validation data, where available, confirm that the recombinant protein retains relevant biological activity.
• Formulation and Storage: Protein formulation, recommended storage temperature, and stability data are provided on the product datasheet. Proper handling is essential to maintain protein integrity.
• Endotoxin Levels: Endotoxin testing is routinely performed on recombinant proteins intended for cell-based assays to minimize experimental interference.

For full specification details including molecular weight, tag information, expression host, and lot-specific quality data, researchers are directed to the official product datasheet available at Aladdin Scientific.

Research Applications

Recombinant Human GIMAP8 Protein is a versatile tool for researchers investigating the GIMAP protein family and immune cell biology. Typical research applications for recombinant proteins of this type include:

• Protein–Protein Interaction Studies: GIMAP8 can be used as bait or prey in pull-down assays, co-immunoprecipitation experiments, or surface plasmon resonance (SPR) studies to identify and characterize interaction partners within the GIMAP family or broader immune signaling networks.
• Structural and Biochemical Studies: Purified recombinant GIMAP8 supports biochemical characterization of its GTPase activity, nucleotide binding properties, and domain function.
• Antibody Development and Validation: Recombinant GIMAP8 protein serves as an immunogen for generating antibodies and as a positive control antigen for ELISA, Western blot, and immunoassay validation.
• Functional Assays: In cell-free or reconstituted systems, recombinant GIMAP8 may be used to investigate its role in apoptotic signaling pathways relevant to lymphocyte biology.
• Drug Discovery: GIMAP8 may serve as a target protein for screening small-molecule modulators of GIMAP family GTPase activity in early-stage drug discovery programs.

Researchers should validate the suitability of this protein for their specific assay format and conditions. Refer to the product datasheet for recommended usage guidance.

Handling, Storage, and Reconstitution Guidelines

Proper handling of recombinant proteins is critical to ensuring experimental reproducibility and maintaining protein activity. The following general guidelines apply to Recombinant Human GIMAP8 Protein; always refer to the product-specific datasheet for authoritative instructions:

• Storage: Recombinant proteins are typically stored at −20 °C or −80 °C upon receipt. Avoid repeated freeze–thaw cycles, as these can degrade protein activity and promote aggregation.
• Reconstitution: If supplied as a lyophilized powder, reconstitute using the buffer specified in the datasheet (commonly sterile PBS or similar physiological buffer). Allow the protein to dissolve gently; do not vortex vigorously.
• Aliquoting: Upon first thaw or reconstitution, aliquot the protein into single-use volumes to minimize freeze–thaw cycling.
• Working Concentrations: Optimize working concentrations empirically for each assay format. Pilot experiments with a dilution series are recommended.
• Carrier Protein: For low-concentration preparations, addition of a carrier protein (e.g., BSA) may be advisable to prevent adsorptive losses; consult the datasheet for formulation details.

Contact Aladdin Scientific technical support for application-specific handling recommendations.

Frequently asked questions

What is the biological function of GIMAP8?

GIMAP8 is a member of the GTPase IMAP (GIMAP) family of small GTPases, which are primarily expressed in immune cells and are implicated in regulating lymphocyte survival, development, and apoptosis. The precise molecular mechanisms of GIMAP8 continue to be an active area of research.

What research applications is Recombinant Human GIMAP8 Protein suitable for?

Recombinant Human GIMAP8 Protein (rp372902) is suitable for protein–protein interaction studies, biochemical characterization of GTPase activity, antibody development and validation, functional assays in cell-free systems, and as a research tool in early-stage drug discovery targeting the GIMAP family. Suitability for specific assay formats should be confirmed experimentally.

How should Recombinant Human GIMAP8 Protein be stored to maintain activity?

The protein should be stored according to the conditions specified in the product datasheet, generally at −20 °C or −80 °C. Repeated freeze–thaw cycles should be avoided by aliquoting the protein into single-use volumes upon first thaw. Always refer to the datasheet for formulation-specific storage recommendations.

Where can I find the Certificate of Analysis (CoA) for this product?

The Certificate of Analysis for Recombinant Human GIMAP8 Protein (rp372902) is available through Aladdin Scientific's website or by contacting customer support with your lot number. The CoA contains lot-specific purity, activity, and quality control data.

Is this recombinant protein validated for use in cell-based assays?

Validation data for specific assay formats are provided in the product datasheet. Researchers are advised to consult the datasheet for confirmed applications and to perform pilot experiments to optimize conditions for their specific cell-based assay systems.

References

1. Cambot M, Aresta S, Kahn-Perles B, de Gunzburg J, Rohrlich PS. Human immune associated nucleotide 1: a member of a new guanosine triphosphatase family expressed in resting CD4+ T cells. Mol Immunol. 2002;39(7-8):457-465.
2. Nitta T, Nasreen M, Seike T, et al. IAN family critically regulates survival and development of T lymphocytes. PLoS Biol. 2006;4(4):e103.
3. Schnell S, Demolliere C, van den Berk P, Bhatt DL. Gimap4 accelerates T-cell death. Blood. 2006;108(2):591-599.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.