Recombinant Human GKN1 Protein: Key FAQs & Specs
Recombinant Human GKN1 Protein: Key FAQs & Specs
Recombinant Human GKN1 Protein is a gastric mucosal protein with established roles in maintaining gastric epithelial homeostasis and putative tumor-suppressive activity in gastric cancer research.
- Product Name
- Recombinant Human GKN1 Protein
- SKU
- rp367954
- Protein Family
- Gastrokine (GKN) family
- Species Origin
- Human (Homo sapiens)
- Primary Research Area
- Gastric biology, oncology, mucosal immunology
- Supplier Reference
- absea#PQ-4641
What Is GKN1 and Why Is It Important?
GKN1 (Gastrokine-1), also known as AMP-18 or CA11, is a secreted protein predominantly expressed in the superficial epithelium of the gastric antrum and body. It belongs to the Gastrokine family of proteins, which are characteristically expressed in normal gastric mucosa. GKN1 plays a critical role in maintaining the integrity and homeostasis of the gastric epithelial lining by promoting cell proliferation, migration, and mucosal restitution after injury.
Importantly, GKN1 expression is markedly reduced or absent in gastric cancer tissue compared with normal gastric epithelium, positioning it as a candidate tumor suppressor in gastric carcinogenesis. Research has also implicated GKN1 in modulating inflammatory responses associated with Helicobacter pylori infection, a major risk factor for gastric cancer. The availability of a high-quality recombinant form of GKN1 enables researchers to study its mechanistic roles in vitro and in vivo without reliance on native tissue extraction.
Product Specifications and Quality Attributes
Aladdin Scientific's Recombinant Human GKN1 Protein (SKU: rp367954) is produced using recombinant expression technology to deliver a well-characterized reagent suitable for demanding research applications. Key quality attributes to confirm with the current lot certificate of analysis include:
- Expression System: Typically mammalian or E. coli-based expression; please consult the current datasheet for lot-specific details.
- Purity: Expected to be >90% by SDS-PAGE; confirm with certificate of analysis.
- Formulation: Typically supplied in a carrier protein-containing buffer; consult current datasheet for exact formulation details.
- Endotoxin Level: Endotoxin testing is recommended for cell-based functional assays; verify with lot-specific certificate of analysis.
- Molecular Weight: Consistent with the theoretical molecular weight of GKN1; confirm with current datasheet.
Researchers should always refer to the current product datasheet and certificate of analysis for the most accurate lot-specific specifications before initiating experiments.
Storage, Handling, and Stability Recommendations
Proper handling and storage of Recombinant Human GKN1 Protein are critical to preserving biological activity and ensuring reproducible experimental results. General best-practice recommendations include:
- Long-term storage: Store at −20°C or −80°C as indicated on the current datasheet. Avoid repeated freeze-thaw cycles, which can significantly reduce protein activity.
- Aliquoting: Upon first thaw, aliquot the protein into single-use volumes appropriate for your experimental needs to minimize freeze-thaw degradation.
- Working solution preparation: Reconstitute lyophilized protein (if applicable) in sterile, carrier-appropriate buffer as directed in the product datasheet. Allow reconstituted protein to equilibrate gently; avoid vigorous vortexing.
- Short-term storage: If repeated use within a short period is planned, store at 4°C for up to the duration specified on the datasheet, typically no more than one week.
- Contamination prevention: Use sterile technique and endotoxin-free consumables, particularly for cell-based assays.
Always consult the current product datasheet for lot-specific storage and handling instructions, as formulation details may vary between lots.
Research Applications of Recombinant Human GKN1 Protein
Recombinant Human GKN1 Protein is used across a range of gastric biology and oncology research applications. Common uses include:
- Cell proliferation and apoptosis assays: Investigating the role of GKN1 in regulating gastric epithelial cell growth and programmed cell death.
- Migration and wound-healing assays: Studying GKN1's contribution to mucosal restitution after epithelial injury.
- Protein–protein interaction studies: Identifying binding partners of GKN1 relevant to its tumor-suppressive function.
- Western blot and ELISA standards: Using recombinant GKN1 as a positive control or calibrator in immunoassays detecting endogenous GKN1.
- Inflammatory signaling research: Examining GKN1's modulatory effects on cytokine production and NF-κB signaling in gastric epithelial cells.
- In vitro tumor suppression models: Evaluating the ability of exogenous GKN1 to suppress proliferation of gastric cancer cell lines.
Researchers should validate the protein's activity in their specific assay system using appropriate positive and negative controls before committing to large-scale experiments.
Ordering, Availability, and Technical Support
Recombinant Human GKN1 Protein is available from Aladdin Scientific under SKU rp367954. To order or request a quote, please use the Aladdin Scientific website or contact your regional Aladdin Scientific representative.
For technical inquiries regarding suitability for specific applications, recommended assay conditions, or troubleshooting, Aladdin Scientific's technical support team is available to assist. When contacting technical support, please have the following information ready:
- Product SKU and lot number (found on the vial label)
- Description of the experimental application and assay system
- Storage and handling conditions used
- Observed issue or question in detail
Bulk quantities and custom formulations may be available upon request. Please contact Aladdin Scientific directly for information on pricing, minimum order quantities, lead times, and available pack sizes for SKU rp367954.
Frequently asked questions
What is the biological function of GKN1 protein?
GKN1 (Gastrokine-1) is a secreted protein expressed predominantly in normal gastric mucosal epithelium. It promotes epithelial cell proliferation, migration, and mucosal repair, and is widely studied for its putative tumor-suppressive role in gastric carcinogenesis, where its expression is frequently lost or reduced.
What expression system is used to produce Recombinant Human GKN1 Protein (rp367954)?
The specific expression system for SKU rp367954 should be confirmed on the current product datasheet and certificate of analysis, as this detail may be updated between lots. Recombinant GKN1 proteins are produced in mammalian or E. coli expression systems depending on the supplier's manufacturing process. Please download the current datasheet from the Aladdin Scientific website or contact technical support for lot-specific information.
How should Recombinant Human GKN1 Protein be stored to maintain activity?
For long-term storage, Recombinant Human GKN1 Protein should be kept at −20°C or −80°C as specified in the current product datasheet. Repeated freeze-thaw cycles should be avoided; aliquoting into single-use volumes upon first thaw is strongly recommended. For short-term use, storage at 4°C is acceptable for up to the duration indicated on the datasheet.
Can Recombinant Human GKN1 Protein be used as a positive control in Western blot or ELISA assays?
Yes, recombinant human GKN1 protein is commonly used as a positive control or standard in Western blot and ELISA-based immunoassays designed to detect endogenous GKN1. Researchers should verify antibody cross-reactivity and optimal loading concentrations in their specific assay format prior to use in quantitative applications.
Is Recombinant Human GKN1 Protein suitable for cell-based functional assays?
Recombinant Human GKN1 Protein can be used in cell-based assays such as proliferation, apoptosis, and migration studies. For cell-based applications, it is important to verify the endotoxin level using the lot-specific certificate of analysis, as endotoxin contamination can confound results. Use of endotoxin-free reagents and sterile handling technique is strongly advised.
How do I place an order for Recombinant Human GKN1 Protein from Aladdin Scientific?
You can order Recombinant Human GKN1 Protein (SKU: rp367954) directly through the Aladdin Scientific website, by contacting your regional Aladdin Scientific sales representative, or by reaching out to the customer service team. For bulk orders or custom formulations, please contact Aladdin Scientific directly to discuss availability, pricing, and lead times.
References
- Nardone G, et al. Gastrokine 1 is a gastric mucosal protein that is downregulated in gastric cancer. General reference for GKN1 biology — specific citation details require verification.
- Martin TE, et al. AMP-18 (GKN1): a gastric-specific secreted protein with roles in mucosal integrity and tumor suppression. General reference — specific citation details require verification.
- Aladdin Scientific. Recombinant Human GKN1 Protein, SKU rp367954. Product datasheet. Aladdin Scientific; [current version].
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.