Recombinant Human GLIS1 Protein: Properties & Research Guide

Recombinant Human GLIS1 Protein is a research-grade recombinant transcription factor derived from the GLI-similar family, relevant to cell reprogramming and developmental biology studies.

Product Name

Recombinant Human GLIS1 Protein

SKU

rp369357

Protein Family

GLI-similar (GLIS) zinc-finger transcription factors

Species Origin

Human (Homo sapiens)

Primary Research Area

Cell reprogramming, developmental biology, transcriptional regulation

Supplier

Aladdin Scientific

Background: GLIS1 Biology and Significance

GLIS1 (GLI-Similar 1) is a member of the GLIS subfamily of Krüppel-like zinc-finger transcription factors, which are closely related to the GLI and ZIC protein families. GLIS proteins contain a highly conserved zinc-finger domain that enables specific DNA binding, allowing them to act as transcriptional activators or repressors depending on cellular context.

GLIS1 has attracted significant research interest due to its role in facilitating somatic cell reprogramming. Studies have shown that GLIS1 can enhance the efficiency of induced pluripotent stem cell (iPSC) generation when co-expressed with classical Yamanaka factors (OCT4, SOX2, KLF4, and c-MYC). This property makes GLIS1 a valuable tool in stem cell biology and regenerative medicine research.

Beyond reprogramming, GLIS1 is implicated in early embryonic development and ciliary signaling pathways, broadening its relevance across multiple fields of biomedical research.

Product Specifications and Quality Attributes

Aladdin Scientific's Recombinant Human GLIS1 Protein (SKU: rp369357) is manufactured to research-grade standards. Researchers should consult the product datasheet for confirmed specifications including molecular weight, expression system, tag information, purity grade, and endotoxin levels, as these parameters are critical for experimental reproducibility.

When selecting a recombinant protein for functional assays, key attributes to verify include:

• Purity: Typically assessed by SDS-PAGE and/or HPLC; high purity (>90%) is recommended for most functional studies.
• Endotoxin content: Low endotoxin levels are essential for cell-based assays to avoid confounding inflammatory responses.
• Biological activity: Functional validation data (e.g., DNA binding, transcriptional reporter assays) confirm lot-to-lot consistency.
• Expression system: The host system (e.g., E. coli, mammalian, insect cells) can influence post-translational modifications and activity.

Please refer to the official product page or contact Aladdin Scientific technical support for the most current certificate of analysis (CoA).

Handling, Reconstitution, and Storage Guidelines

Proper handling of recombinant proteins is essential to preserve biological activity and ensure reproducible experimental results. The following general guidelines apply to Recombinant Human GLIS1 Protein; always cross-reference with the product-specific datasheet.

Reconstitution

• Briefly centrifuge the vial before opening to collect lyophilized material at the bottom.
• Reconstitute in the buffer specified in the datasheet (typically sterile PBS or Tris-based buffer with a carrier protein such as BSA).
• Allow the protein to dissolve gently; avoid vigorous vortexing, which can cause denaturation.

Storage

• Store lyophilized protein at –20 °C or –80 °C as specified. Avoid repeated freeze-thaw cycles.
• After reconstitution, prepare single-use aliquots and store at –80 °C for long-term use, or at 4 °C for short-term use (typically up to 1 week).
• Adding a carrier protein (e.g., 0.1% BSA) to the reconstitution buffer can improve stability.

General Precautions

• Handle on ice whenever possible during working sessions.
• Use low-binding microcentrifuge tubes to minimize protein adsorption losses.
• Record lot number and reconstitution date on each aliquot for traceability.

Research Applications of GLIS1 Protein

Recombinant Human GLIS1 Protein supports a range of in vitro research applications. Representative uses include:

• Induced Pluripotent Stem Cell (iPSC) Research: GLIS1 has been reported to enhance reprogramming efficiency in combination with Yamanaka factors, making it relevant for iPSC generation and optimization studies.
• Transcriptional Regulation Studies: As a zinc-finger transcription factor, GLIS1 can be used in DNA-binding assays, electrophoretic mobility shift assays (EMSA), and reporter gene experiments to characterize its target gene networks.
• Developmental Biology: GLIS1 expression in early embryos and its association with ciliary function make it a subject of study in developmental signaling research.
• Protein–Protein Interaction Studies: Co-immunoprecipitation (Co-IP) and pull-down assays using recombinant GLIS1 can help identify interaction partners in transcriptional complexes.
• Antibody Validation: Recombinant GLIS1 protein serves as a positive control antigen for validating anti-GLIS1 antibodies in Western blot and ELISA.

Researchers are encouraged to optimize assay conditions empirically and consult the datasheet for recommended working concentrations.

Ordering and Technical Support

Recombinant Human GLIS1 Protein is available from Aladdin Scientific under SKU rp369357. To place an order or request a quotation, visit the Aladdin Scientific website or contact your regional sales representative.

For technical inquiries—including questions about reconstitution, experimental design, or compatibility with specific assay formats—Aladdin Scientific's technical support team is available to assist. Please have the product SKU and lot number ready when contacting support to facilitate efficient assistance.

Bulk quantities, custom formulations, or special packaging requests may be accommodated; contact sales for details. Researchers should also review the Safety Data Sheet (SDS) available on the product page before use.

Frequently asked questions

What is GLIS1 and why is it important in research?

GLIS1 is a member of the GLI-similar zinc-finger transcription factor family involved in transcriptional regulation, early embryonic development, and ciliary signaling. It has gained prominence in stem cell research for its ability to enhance the efficiency of induced pluripotent stem cell (iPSC) generation when used alongside classical reprogramming factors.

How should Recombinant Human GLIS1 Protein be stored after reconstitution?

After reconstitution, the protein should be aliquoted into single-use volumes and stored at –80 °C for long-term stability. For short-term use within one week, storage at 4 °C is generally acceptable. Repeated freeze-thaw cycles should be avoided as they can reduce protein activity. Always consult the product datasheet for lot-specific storage recommendations.

What applications is Recombinant Human GLIS1 Protein validated for?

Recombinant Human GLIS1 Protein (rp369357) is intended for research applications including transcription factor binding assays, iPSC reprogramming studies, protein interaction experiments, and antibody validation as a positive control antigen. Specific validated applications and recommended working concentrations are detailed in the product datasheet; researchers should verify suitability for their particular assay format.

How can I obtain a Certificate of Analysis (CoA) for this product?

The Certificate of Analysis for Recombinant Human GLIS1 Protein (rp369357) can be obtained through the Aladdin Scientific product page by entering the lot number, or by contacting Aladdin Scientific customer or technical support directly. Always use the CoA corresponding to your specific lot number for accurate specification data.

Can GLIS1 protein be used in cell-based assays?

Recombinant GLIS1 protein can potentially be used in cell-based experiments; however, endotoxin levels must be verified to be sufficiently low to avoid non-specific inflammatory responses that could confound results. Researchers should consult the datasheet for endotoxin specifications and perform empirical optimization for their specific cell model and assay conditions.

References

1. Maekawa M, et al. Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Nature. 2011.
2. Zhang F, et al. GLIS family genes in development and disease. General review reference — details to be confirmed.
3. Aladdin Scientific. Recombinant Human GLIS1 Protein, SKU rp369357. Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.