Recombinant Human GNAT3 Protein: Key Facts & FAQs

Recombinant Human GNAT3 Protein is a research-grade recombinant form of the guanine nucleotide-binding protein G(t) subunit alpha-3, also known as gustducin alpha subunit, produced for use in biochemical and cell biology studies of taste signal transduction.

Product Name

Recombinant Human GNAT3 Protein

SKU

rp372304

Gene / Protein

GNAT3 (Guanine nucleotide-binding protein G(t) subunit alpha-3; Alpha-gustducin)

Supplier Reference

absea#PP-11772

Protein Class

Heterotrimeric G protein alpha subunit

Primary Research Area

Taste signal transduction, chemosensory biology

What Is GNAT3 and Why Is It Important?

GNAT3 encodes the alpha subunit of gustducin, a heterotrimeric G protein expressed predominantly in taste receptor cells of the tongue and palate. It plays a central role in the intracellular signaling cascade that mediates the perception of bitter, sweet, and umami tastes. Upon activation by taste GPCRs (such as T1Rs and T2Rs), gustducin alpha (GNAT3) dissociates from the beta-gamma subunits and modulates downstream effectors including phosphodiesterases and phospholipase C, ultimately leading to changes in intracellular cyclic nucleotide and calcium levels that trigger neuronal signaling to the brain.

Beyond canonical taste tissue, GNAT3 has been detected in extraoral chemosensory cells of the gut, airway epithelium, and pancreas, suggesting broader roles in nutrient sensing and metabolic regulation. This makes recombinant GNAT3 protein a valuable reagent for researchers investigating G protein function, chemosensory signaling, and metabolic disease mechanisms.

Product Specifications and Quality

Recombinant Human GNAT3 Protein (SKU: rp372304) is produced as a recombinant protein intended for research use. The product is referenced against supplier catalogue entry absea#PP-11772. As with all Aladdin Scientific recombinant proteins, this product is manufactured under controlled conditions designed to deliver consistent lot-to-lot quality suitable for demanding biochemical assays.

Key specification details to confirm with the product datasheet or Aladdin Scientific technical support include:

• Expression host system (e.g., E. coli, baculovirus/insect cells, or mammalian cells)
• Protein purity (typically assessed by SDS-PAGE and/or HPLC)
• Biological activity or functional validation data
• Tag information (e.g., His-tag, GST-tag) if applicable
• Formulation buffer and recommended storage conditions
• Endotoxin level (relevant for cell-based assays)

Please refer to the official product datasheet for lot-specific certificate of analysis (CoA) data, or contact Aladdin Scientific technical support for detailed specifications.

Recommended Applications

Recombinant Human GNAT3 Protein is suitable for a range of in vitro research applications. Typical uses include:

• Biochemical binding assays: Studying protein-protein interactions between GNAT3 and taste GPCRs, beta-gamma subunits, or downstream effectors such as phosphodiesterase.
• GTPase activity assays: Measuring intrinsic or receptor-stimulated GTP hydrolysis activity of the alpha subunit.
• Western blot (WB) standard: Use as a positive control or molecular weight standard in immunoblotting experiments.
• ELISA and immunoassay development: Serving as an antigen standard for antibody validation or sandwich ELISA development.
• Structural and biophysical studies: Supporting crystallography, cryo-EM, or SPR experiments to characterize GNAT3 conformation and interactions.
• Drug discovery: Screening small molecules that modulate gustducin signaling for therapeutic applications in metabolic or taste-related disorders.

Researchers should validate the protein's suitability for each specific application and refer to the product datasheet for any application-specific notes.

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins is essential to maintain activity and integrity. While lot-specific conditions are provided in the product datasheet, general best-practice guidelines for recombinant proteins like GNAT3 include:

• Short-term storage: Store at 4 °C for up to one week if the protein will be used promptly after receipt.
• Long-term storage: Aliquot into single-use volumes and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles, which can cause protein aggregation and loss of activity.
• Carrier protein: Addition of a stabilizing carrier such as 0.1% BSA is recommended for dilute protein solutions to prevent adsorption to tube surfaces.
• Avoid repeated freeze-thaw cycles: Each freeze-thaw cycle can degrade protein quality; aliquoting prior to freezing is strongly advised.
• Working solutions: Prepare fresh dilutions on ice and keep on ice during use.

Always consult the specific CoA and datasheet supplied with each lot for authoritative storage and handling instructions.

Ordering and Technical Support

Recombinant Human GNAT3 Protein is available from Aladdin Scientific under SKU rp372304. To place an order or request a quotation, visit the Aladdin Scientific website or contact your regional sales representative.

For technical questions regarding experimental design, application suitability, or troubleshooting, Aladdin Scientific's technical support team is available to assist. When contacting support, please have the following information ready:

• Product SKU: rp372304
• Lot number (found on the product label and CoA)
• Description of the application and assay conditions
• Any troubleshooting data or observations

Bulk quantities, custom formulations, or special packaging requirements may be accommodated upon request—please contact Aladdin Scientific directly to discuss your project needs.

Frequently asked questions

What is GNAT3 and what biological role does it play?

GNAT3 encodes the alpha subunit of gustducin, a G protein expressed in taste receptor cells that mediates intracellular signaling for bitter, sweet, and umami taste perception. It activates downstream effectors such as phosphodiesterases and phospholipase C to modulate cyclic nucleotide and calcium levels, ultimately generating a neural signal interpreted as taste. GNAT3 has also been identified in extraoral chemosensory tissues including the gut and airway, implicating it in broader nutrient-sensing and metabolic functions.

What expression system is used to produce Recombinant Human GNAT3 Protein (rp372304)?

The specific expression host system for SKU rp372304 is detailed in the product datasheet and certificate of analysis (CoA) provided with each lot. Common expression systems for recombinant G protein subunits include E. coli, insect cells (baculovirus), and mammalian cell lines. Please download the datasheet from the Aladdin Scientific website or contact technical support for confirmed expression system information for this product.

What applications is Recombinant Human GNAT3 Protein validated for?

Recombinant Human GNAT3 Protein is suitable for a range of in vitro research applications, including biochemical binding assays, GTPase activity measurements, Western blot positive controls, ELISA antigen standards, and structural or biophysical studies. It may also be useful in drug discovery screening for modulators of gustducin signaling. Validated applications for each lot are specified in the accompanying datasheet; researchers should confirm suitability for their specific assay conditions.

How should Recombinant Human GNAT3 Protein be stored to maintain stability?

For short-term use, the protein may be stored at 4 °C for up to one week. For long-term storage, aliquot into single-use volumes and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles. Adding a carrier protein such as 0.1% BSA is recommended for dilute solutions. Always refer to the specific CoA and product datasheet for lot-authoritative storage conditions.

How can I order Recombinant Human GNAT3 Protein or request a custom quote?

Recombinant Human GNAT3 Protein is available under SKU rp372304 on the Aladdin Scientific website. You can place an order directly online, contact your regional Aladdin Scientific sales representative, or reach out to the customer service team for bulk orders, custom formulations, or special packaging requirements.

References

1. McLaughlin SK, McKinnon PJ, Margolskee RF. Gustducin is a taste-cell-specific G protein closely related to the transducins. Nature. 1992;357(6379):563-569. doi:10.1038/357563a0
2. Margolskee RF. Molecular mechanisms of bitter and sweet taste transduction. J Biol Chem. 2002;277(1):1-4. doi:10.1074/jbc.R100054200
3. Rozengurt N, Wu SV, Chen MC, et al. Colocalization of the alpha-subunit of gustducin with PYY and GLP-1 in L cells of human colon. Am J Physiol Gastrointest Liver Physiol. 2006;291(5):G792-G802. doi:10.1152/ajpgi.00222.2006

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.