Recombinant Human GNB1L Protein: Key Facts & FAQs

Recombinant Human GNB1L Protein is a research-grade recombinant protein derived from the human GNB1L gene, offered by Aladdin Scientific (SKU: rp372907) to support studies in G protein signaling, neurodevelopmental biology, and related biomedical research applications.

Product Name

Recombinant Human GNB1L Protein

SKU

rp372907

Gene Name

GNB1L (G Protein Subunit Beta 1 Like)

Species

Human (Homo sapiens)

Protein Family

WD repeat-containing protein; G protein beta subunit-like family

Research Application

Biochemical assays, protein interaction studies, neurodevelopmental research

Supplier

Aladdin Scientific

What Is GNB1L and Why Is It Important?

GNB1L (G Protein Subunit Beta 1 Like) is a human gene encoding a WD repeat-containing protein that shares structural homology with the beta subunits of heterotrimeric G proteins. Despite its similarity to classical Gβ subunits, GNB1L does not function as a canonical signal-transducing G protein beta subunit; instead, it is implicated in vesicular trafficking, receptor recycling, and intracellular signaling scaffolding.

GNB1L is located within the 22q11.2 chromosomal region, a locus associated with 22q11.2 deletion syndrome (DiGeorge syndrome/velocardiofacial syndrome). Haploinsufficiency of genes in this region, potentially including GNB1L, has been linked to neurodevelopmental and psychiatric phenotypes, making GNB1L a subject of active investigation in the fields of schizophrenia research and developmental neurobiology.

Product Overview: Recombinant Human GNB1L Protein (rp372907)

Aladdin Scientific's Recombinant Human GNB1L Protein (SKU: rp372907) is produced using recombinant expression technology to provide researchers with a reliable, consistent source of GNB1L protein for in vitro studies. Recombinant proteins of this type are typically produced in bacterial, insect, or mammalian expression systems to achieve appropriate folding and post-translational modifications where required.

This reagent is intended for research use only and is not approved for diagnostic or therapeutic applications. Researchers should consult the product datasheet for confirmed purity, expression system details, formulation, and recommended storage conditions prior to use.

Note: Detailed specifications such as purity percentage, expression host, molecular weight confirmation, and endotoxin levels should be verified against the official Aladdin Scientific product datasheet for SKU rp372907.

Recommended Applications for GNB1L Recombinant Protein

Recombinant Human GNB1L Protein is suited for a range of biochemical and cell biology research applications, including but not limited to:

• Protein–protein interaction studies: Identifying binding partners of GNB1L using pull-down assays, co-immunoprecipitation validation, or surface plasmon resonance (SPR).
• Antibody validation: Serving as a positive control antigen for validating anti-GNB1L antibodies in Western blotting or ELISA.
• Enzyme-linked immunosorbent assay (ELISA) standards: Use as a calibration standard in quantitative GNB1L detection assays.
• Structural and biophysical studies: Supporting X-ray crystallography, cryo-EM, or other structural biology approaches when sufficient quantities are available.
• Functional assays: Investigating GNB1L's role in vesicular trafficking or receptor signaling pathways in cell-free or reconstituted systems.

Always refer to the product datasheet for validated application data specific to this lot and SKU.

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins are critical to maintaining activity and integrity. As a general guideline for recombinant proteins of this class:

• Storage temperature: Store at –20°C or –80°C for long-term stability. Avoid repeated freeze–thaw cycles, which can lead to protein aggregation and loss of activity.
• Aliquoting: Upon first thaw, aliquot the protein into single-use volumes to minimise freeze–thaw cycling.
• Buffer/formulation: Refer to the official product datasheet for the exact formulation, carrier proteins, and stabilisers included.
• Sterility: Handle under aseptic conditions if the protein is to be used in cell-based assays.
• Expiry: Check the certificate of analysis (CoA) for lot-specific expiry information.

Important: Specific storage conditions for rp372907 must be confirmed from the Aladdin Scientific product datasheet or CoA, as formulations may vary by lot.

Ordering and Technical Support

Recombinant Human GNB1L Protein is available from Aladdin Scientific under SKU rp372907. To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative.

For technical questions regarding experimental design, recommended concentrations, or compatibility with downstream assays, please contact Aladdin Scientific's technical support team. Provide your SKU, lot number, and a description of your experimental system for the most accurate guidance.

Bulk quantities or custom formulations may be available upon request — contact Aladdin Scientific customer service for details on custom protein services.

Frequently asked questions

What is GNB1L and what does the protein do?

GNB1L encodes a WD repeat-containing protein with structural homology to G protein beta subunits. It is implicated in intracellular vesicular trafficking and receptor recycling pathways. GNB1L resides within the 22q11.2 chromosomal region and is of particular interest in neurodevelopmental and psychiatric disease research.

What expression system is used to produce rp372907?

The specific expression host (e.g., E. coli, insect cells, or HEK293 mammalian cells) for SKU rp372907 should be confirmed by consulting the official Aladdin Scientific product datasheet or certificate of analysis. Different expression systems affect post-translational modifications and protein folding, so this information is important for experimental planning.

How should I store Recombinant Human GNB1L Protein to maintain activity?

As a general best practice for recombinant proteins, store at –20°C or –80°C and avoid repeated freeze–thaw cycles by aliquoting the protein upon first thaw. Always verify the exact recommended storage conditions, formulation, and shelf life from the product datasheet or CoA for SKU rp372907.

What research applications is Recombinant Human GNB1L Protein suitable for?

This protein is suitable for a variety of in vitro research applications including protein–protein interaction studies, antibody validation as a positive control antigen, ELISA calibration standards, and structural biology investigations. It may also be used in functional assays studying vesicular trafficking or G protein-related signaling. Refer to the datasheet for validated application-specific data.

How can I order Recombinant Human GNB1L Protein from Aladdin Scientific?

You can order Recombinant Human GNB1L Protein using SKU rp372907 directly through the Aladdin Scientific website or by contacting your regional sales representative. For bulk quantities or custom formulations, please reach out to Aladdin Scientific customer service.

References

1. Morrow B et al. Molecular definition of the 22q11 deletions in velo-cardio-facial syndrome. American Journal of Human Genetics. 1995.
2. Aladdin Scientific Product Datasheet: Recombinant Human GNB1L Protein, SKU rp372907. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.