Recombinant Human GNPDA1 Protein: Key Facts & FAQs

Recombinant Human GNPDA1 Protein is a recombinant form of the human glucosamine-6-phosphate deaminase 1 enzyme, produced for use in biochemical research into amino sugar metabolism and related cellular pathways.

Product Name

Recombinant Human GNPDA1 Protein

SKU

rp369595

Protein Function

GNPDA1 (Glucosamine-6-Phosphate Deaminase 1) catalyzes the conversion of glucosamine-6-phosphate to fructose-6-phosphate and ammonium, a key step in amino sugar catabolism.

Species Origin

Human (Homo sapiens)

Supplier Reference

absea#PP-3948

Intended Use

Research use only; suitable for biochemical assays, enzyme activity studies, and metabolic pathway investigations.

What Is GNPDA1 and Why Is It Important in Research?

GNPDA1, or Glucosamine-6-Phosphate Deaminase 1, is an enzyme that plays a central role in amino sugar metabolism. It catalyzes the reversible isomerization and deamination of glucosamine-6-phosphate to produce fructose-6-phosphate and ammonium ions. This reaction sits at a critical junction between the hexosamine biosynthetic pathway and glycolysis, making GNPDA1 relevant to studies of glucose metabolism, cellular energy regulation, and glycoprotein biosynthesis.

Research interest in GNPDA1 has grown in the context of metabolic diseases, cancer biology, and nutrient sensing. As a recombinant protein, it enables researchers to conduct in vitro enzyme activity assays, protein interaction studies, and structural investigations without the variability associated with native tissue extraction.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human GNPDA1 Protein (SKU: rp369595) is manufactured with research-grade quality standards. The product is referenced under supplier code absea#PP-3948. Key specification details including exact molecular weight, expression host system, tag information, purity percentage, endotoxin levels, and formulation buffer should be confirmed directly from the product datasheet available on the Aladdin Scientific website or by contacting technical support, as precise values are subject to lot-specific certificates of analysis.

In general, recombinant proteins of this class are commonly expressed in E. coli or mammalian/insect cell systems and are purified to research-grade purity, typically assessed by SDS-PAGE and/or HPLC. Researchers are encouraged to request the most current datasheet and CoA for lot-specific data.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human GNPDA1 Protein are essential to maintain its activity and integrity. As a general guideline for recombinant proteins:

• Upon receipt, briefly centrifuge the vial before opening to consolidate the contents.
• For short-term storage (up to one week), keep at 4°C.
• For long-term storage, aliquot the protein into single-use volumes and store at −20°C or −80°C to avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of enzymatic activity.
• Addition of a carrier protein (e.g., 0.1% BSA) may help stabilize the protein during storage at low concentrations.

Always refer to the product-specific datasheet for confirmed storage recommendations, as conditions may vary by formulation lot.

Recommended Applications

Recombinant Human GNPDA1 Protein is suitable for a range of in vitro research applications, including but not limited to:

• Enzyme Activity Assays: Measuring deaminase activity in the conversion of glucosamine-6-phosphate to fructose-6-phosphate.
• Protein–Protein Interaction Studies: Identifying binding partners of GNPDA1 using pull-down or co-immunoprecipitation approaches.
• Structural Biology: Used as a reference or substrate in crystallography or cryo-EM studies.
• Drug Discovery and Screening: Serving as a target protein for inhibitor or activator screening in metabolic disease research.
• Western Blot Standards: As a positive control or molecular weight reference in immunoblotting experiments.

Researchers should validate the protein's performance in their specific assay systems and refer to the product datasheet for confirmed application recommendations.

Ordering and Technical Support

Recombinant Human GNPDA1 Protein is available from Aladdin Scientific under SKU rp369595. To place an order, visit the Aladdin Scientific website and search by SKU or product name. Bulk quantities, custom formulations, or additional lot-specific documentation such as Certificates of Analysis (CoA) and Safety Data Sheets (SDS) can be requested through the customer service team.

For technical inquiries regarding experimental design, compatibility, or troubleshooting, researchers are encouraged to contact Aladdin Scientific's technical support team directly. Our specialists can assist with application-specific guidance to help ensure experimental success.

Frequently asked questions

What is the biological function of GNPDA1?

GNPDA1 (Glucosamine-6-Phosphate Deaminase 1) catalyzes the reversible deamination of glucosamine-6-phosphate into fructose-6-phosphate and ammonium. This reaction links the hexosamine biosynthetic pathway to glycolysis and is important for amino sugar catabolism and cellular metabolic regulation.

What expression system is used to produce Recombinant Human GNPDA1 Protein?

The specific expression host system for SKU rp369595 should be confirmed via the official product datasheet or Certificate of Analysis available from Aladdin Scientific. Common systems for recombinant proteins of this type include E. coli, baculovirus/insect cells, or mammalian cell expression platforms.

How should Recombinant Human GNPDA1 Protein be stored to preserve activity?

For short-term use, store at 4°C for up to one week. For long-term storage, aliquot into single-use volumes and store at −20°C or −80°C to avoid repeated freeze-thaw cycles. Always consult the product datasheet for lot-specific storage conditions.

What applications is Recombinant Human GNPDA1 Protein validated for?

This protein is intended for research use and is suitable for applications including enzyme activity assays, protein–protein interaction studies, drug screening, and use as a Western blot standard. Researchers should validate performance in their specific assay systems and consult the product datasheet for confirmed applications.

How can I request a Certificate of Analysis or Safety Data Sheet for this product?

Certificates of Analysis (CoA) and Safety Data Sheets (SDS) for Recombinant Human GNPDA1 Protein (rp369595) can be requested through the Aladdin Scientific website or by contacting our customer service team directly. Please have your lot number ready when making the request.

References

1. Wolosker H, Kline D, Bian Y, Blackshaw S, Cameron AM, Fralich TJ, Schnaar RL, Snyder SH. Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and kidney collecting tubules. FASEB J. 1998.
2. Aladdin Scientific Product Page: Recombinant Human GNPDA1 Protein, SKU rp369595. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.