Recombinant Human GOLGA2P5 Protein: Production & Usage Protocol

Recombinant Human GOLGA2P5 Protein is a research-grade recombinant protein derived from the GOLGA2P5 locus, offered by Aladdin Scientific to support investigations into Golgi apparatus biology, autoantigen characterization, and related cellular trafficking studies.

Product Name

Recombinant Human GOLGA2P5 Protein

SKU

rp376062

Protein Family

Golgin / GOLGA2 pseudogene-related family

Species

Human (Homo sapiens)

Expression System

Recombinant (specific host system to be confirmed with datasheet)

Intended Use

Research use only, not for diagnostic or therapeutic applications

Storage Recommendation

Refer to product datasheet; typically -20°C or -80°C, avoid repeated freeze-thaw cycles

Product Overview and Background

GOLGA2P5 (Golgi Autoantigen, Golgin Subfamily A Member 2 Pseudogene 5) is a pseudogene locus related to the GOLGA2 gene family, which encodes golgins — a class of coiled-coil proteins associated with the Golgi apparatus membrane. Golgins play critical roles in vesicle tethering, Golgi stack maintenance, and intracellular trafficking.

Recombinant Human GOLGA2P5 Protein (SKU: rp376062) is produced for research purposes to facilitate studies into Golgi-associated biology, pseudogene-derived protein expression, and potential autoantigen activity. This product is manufactured to research-grade standards and is intended exclusively for in vitro laboratory use.

Note: Researchers should consult the official product datasheet for confirmed expression host, tag information, molecular weight, purity grade, and specific lot data. All technical specifications should be verified against the current certificate of analysis (CoA).

Materials Required

Before beginning, ensure the following materials are available:

• Recombinant Human GOLGA2P5 Protein (rp376062), Aladdin Scientific
• Sterile deionized water or recommended reconstitution buffer (per datasheet)
• Microcentrifuge tubes (1.5 mL, low-protein-binding recommended)
• Calibrated micropipettes and sterile tips
• Benchtop microcentrifuge
• SDS-PAGE or Western blot apparatus (for quality verification)
• BCA or Bradford protein assay kit (for concentration verification)
• Appropriate storage vials for aliquots
• Ice or cold block for handling on ice
• Personal protective equipment (PPE): lab coat, gloves, eye protection

Reconstitution and Handling Protocol

Follow these steps to safely and effectively reconstitute Recombinant Human GOLGA2P5 Protein:

1. Equilibrate: Remove the vial from storage and allow it to equilibrate to room temperature for approximately 5–10 minutes. Do not expose to prolonged ambient conditions.
2. Centrifuge: Briefly centrifuge the vial (1,000–2,000 × g, 30 seconds) to consolidate lyophilized material at the bottom of the tube, if applicable.
3. Reconstitute: Add the volume of sterile reconstitution buffer specified on the product datasheet. Gently pipette up and down to dissolve; do not vortex, as this may denature the protein.
4. Incubate: Allow the reconstituted solution to stand on ice for 5 minutes to ensure complete dissolution.
5. Verify: Confirm protein concentration using a validated assay (e.g., BCA assay) and verify integrity by SDS-PAGE if required for your application.
6. Aliquot: Divide into single-use aliquots to avoid repeated freeze-thaw cycles, which degrade protein activity and integrity.
7. Store: Store aliquots according to the datasheet recommendation, typically at -20°C for short-term or -80°C for long-term storage.

Important: Specific reconstitution volumes, buffer composition, and working concentrations must be confirmed from the current product datasheet and CoA supplied with each lot.

Suggested Experimental Applications

Recombinant Human GOLGA2P5 Protein may be suitable for the following research applications, subject to confirmation with the product datasheet:

• Western Blot (WB): Use as a positive control antigen or for antibody validation targeting GOLGA2P5 or related golgin epitopes.
• ELISA: Employ as a coating antigen for the detection of anti-GOLGA2P5 antibodies in serum samples or for assay development.
• Protein–Protein Interaction Studies: Investigate binding partners within the golgin interaction network or Golgi trafficking machinery using pull-down or co-immunoprecipitation assays.
• Autoantigen Research: Support studies examining autoimmune responses associated with Golgi antigens.
• Structural Studies: Provide recombinant material for biophysical characterization including circular dichroism (CD) or dynamic light scattering (DLS).

Disclaimer: Application suitability should be independently validated by the end user. This product is for research use only and is not intended for clinical, diagnostic, or therapeutic use.

Quality Control and Troubleshooting

Quality Control: Each lot of Recombinant Human GOLGA2P5 Protein is manufactured to research-grade standards. Users should refer to the certificate of analysis (CoA) provided with each shipment for lot-specific purity, concentration, and QC data. Standard QC methods may include SDS-PAGE and, where applicable, activity assays.

Troubleshooting Guide:

Issue	Possible Cause	Recommended Action
Protein precipitation upon reconstitution	Incorrect buffer or temperature	Use recommended buffer; reconstitute at 4°C if indicated on datasheet
Low signal in WB or ELISA	Protein degradation or insufficient loading	Check storage history; confirm concentration by BCA; avoid freeze-thaw cycles
Multiple bands on SDS-PAGE	Degradation or aggregation	Inspect CoA; ensure protein was not exposed to repeated freeze-thaw; contact Aladdin Scientific technical support
No detectable protein activity	Denaturation during handling	Handle on ice; do not vortex; confirm reconstitution buffer compatibility

For additional technical support, contact Aladdin Scientific customer service referencing SKU rp376062.

Frequently asked questions

What is GOLGA2P5 and why is it relevant to research?

GOLGA2P5 is a pseudogene locus related to the GOLGA2 gene, which encodes a golgin protein associated with the Golgi apparatus. Research into pseudogene-derived proteins and Golgi autoantigens has implications for understanding intracellular trafficking, Golgi structure maintenance, and autoimmune disease mechanisms. Recombinant protein tools like rp376062 enable in vitro characterization of these functions.

How should I store Recombinant Human GOLGA2P5 Protein to maintain stability?

For optimal stability, store the protein according to the conditions specified on the product datasheet, typically at -20°C for short-term use or -80°C for long-term storage. Always aliquot the protein into single-use volumes upon first thaw to prevent repeated freeze-thaw cycles, which can degrade protein integrity and activity.

What reconstitution buffer should I use for this protein?

The recommended reconstitution buffer and volume are specified on the product datasheet and certificate of analysis (CoA) included with each shipment. Generally, sterile deionized water or a phosphate-buffered saline (PBS)-based buffer is used, but you must confirm the exact recommendation for lot rp376062 by consulting the supplied documentation or contacting Aladdin Scientific technical support.

Can this protein be used for in vivo or clinical applications?

No. Recombinant Human GOLGA2P5 Protein (rp376062) is supplied for research use only and has not been validated for in vivo, diagnostic, or therapeutic applications. Any use outside of in vitro laboratory research is outside the intended scope of this product.

How can I verify the purity and identity of the protein upon receipt?

Upon receipt, review the certificate of analysis (CoA) provided with the shipment for lot-specific purity data. You can independently verify protein identity and purity by running SDS-PAGE under reducing conditions and comparing observed molecular weight with the expected value stated on the datasheet. Western blot with a validated anti-GOLGA2P5 or anti-tag antibody can further confirm identity.

References

1. Aladdin Scientific. Recombinant Human GOLGA2P5 Protein, SKU rp376062. Product Datasheet. Aladdin Scientific.
2. Munro S. The golgin coiled-coil proteins of the Golgi apparatus. Cold Spring Harb Perspect Biol. 2011;3(6):a005256. doi:10.1101/cshperspect.a005256
3. Gillingham AK, Munro S. Finding the Golgi: Golgin coiled-coil proteins show the way. Trends Cell Biol. 2016;26(6):399-408. doi:10.1016/j.tcb.2016.02.005

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.