Recombinant Human GOLGA7 Protein: Key Facts & FAQs

Recombinant Human GOLGA7 Protein is a recombinant form of the human Golgi autoantigen, golgin subfamily a member 7, produced for use as a research reagent in studies of Golgi apparatus structure, membrane trafficking, and related cellular processes.

Product Name

Recombinant Human GOLGA7 Protein

SKU

rp372892

Gene / Protein Target

GOLGA7 (Golgin subfamily A member 7)

Species Origin

Human (Homo sapiens)

Protein Family

Golgi autoantigen / Golgin family

Intended Use

Research use only (RUO)

What Is GOLGA7 and Why Is It Important?

GOLGA7 (Golgi Autoantigen, Golgin Subfamily A, Member 7) is a member of the golgin family of coiled-coil proteins that are predominantly localized to the Golgi apparatus. Golgins play critical structural roles in maintaining Golgi morphology and facilitating vesicular trafficking between Golgi cisternae and other cellular compartments.

GOLGA7 has been identified as a component involved in the tethering of transport vesicles and in regulating the dynamic architecture of the Golgi ribbon. Research has also implicated GOLGA7 in protein palmitoylation pathways, as it interacts with DHHC-type palmitoyltransferases at the Golgi membrane, making it relevant to studies of lipid modification of proteins.

Understanding GOLGA7 function contributes to broader research into Golgi biogenesis, membrane trafficking disorders, and diseases linked to Golgi dysfunction.

Product Specifications and Quality

Recombinant Human GOLGA7 Protein (SKU: rp372892) is produced using recombinant expression technology to deliver a well-characterized research-grade protein. Key specification considerations for this class of recombinant protein typically include:

• Expression System: Recombinant proteins in this category are commonly expressed in bacterial (E. coli), insect (baculovirus/Sf9), mammalian (HEK293, CHO), or wheat germ cell-free systems depending on the folding and post-translational modification requirements of the target protein.
• Purity: Research-grade recombinant proteins are generally supplied at >85% or >95% purity as assessed by SDS-PAGE, with endotoxin levels controlled for cell-based assays.
• Formulation: Typically supplied lyophilized or in aqueous buffer containing stabilizers such as BSA or glycerol to maintain protein integrity during storage and shipping.
• Tag: A purification tag (e.g., His-tag, GST-tag) may be present to facilitate affinity purification; refer to the product datasheet for the specific tag configuration of rp372892.

For confirmed, lot-specific specifications including exact molecular weight, purity data, and formulation details, please refer to the Certificate of Analysis (CoA) provided with your order or contact Aladdin Scientific technical support.

Recommended Research Applications

Recombinant Human GOLGA7 Protein is suitable for a range of in vitro research applications. Common applications for recombinant golgin-family proteins include:

• Protein–Protein Interaction Studies: Used in pull-down assays, co-immunoprecipitation validation, and yeast two-hybrid confirmation to map interaction networks involving GOLGA7 and its binding partners such as DHHC palmitoyltransferases.
• Biochemical Assays: Suitable as a positive control or substrate in enzyme activity assays relevant to palmitoylation or Golgi-associated modifications.
• Antibody Validation: Can be used as an antigen standard for ELISA, Western blot, and dot blot assays to validate anti-GOLGA7 antibody specificity and sensitivity.
• Structural Studies: May be used in biophysical characterization techniques such as circular dichroism (CD), dynamic light scattering (DLS), or as reference material in cryo-EM studies of Golgi tethering complexes.
• Cell-Free Reconstitution Experiments: Applicable in reconstitution assays studying vesicle tethering or Golgi membrane dynamics in vitro.

Researchers should optimize protein concentrations and assay conditions empirically for each specific application.

Storage, Handling, and Reconstitution

Proper storage and handling of Recombinant Human GOLGA7 Protein are essential to preserve its activity and integrity throughout the product's shelf life.

• Storage Temperature: Recombinant proteins are typically stored at –20 °C for short-term use or –80 °C for long-term storage. Avoid repeated freeze–thaw cycles, which can cause protein aggregation and loss of activity. Aliquoting upon first thaw is strongly recommended.
• Reconstitution: If supplied lyophilized, reconstitute with the volume of sterile water, PBS, or buffer specified in the product datasheet. Allow the lyophilized cake to dissolve completely before use. Centrifuge briefly to collect contents before opening the vial.
• Working Conditions: Keep protein on ice during experimental procedures. Avoid prolonged exposure to room temperature.
• Carrier Protein: For dilute working concentrations, addition of a carrier protein (e.g., 0.1% BSA) to the diluent buffer can help prevent non-specific adsorption to tube surfaces.

Always consult the specific product datasheet and Certificate of Analysis supplied with rp372892 for lot-specific storage and handling instructions, as formulations may vary between lots.

Ordering, Support, and Documentation

Recombinant Human GOLGA7 Protein is available exclusively from Aladdin Scientific under SKU rp372892. The following resources and services are available to support your purchase and experimental work:

• Datasheet and CoA: A detailed product datasheet and lot-specific Certificate of Analysis are available upon request or can be downloaded from the product page on the Aladdin Scientific website.
• Technical Support: Aladdin Scientific's technical team is available to assist with application recommendations, reconstitution guidance, and troubleshooting. Contact us through the website support portal or by email.
• Ordering: Place orders directly through the Aladdin Scientific online store or through your institutional procurement system. Bulk quantities and custom formulations may be available upon inquiry.
• Returns and Refunds: If you experience product quality issues, please contact customer service within the return window specified in Aladdin Scientific's returns policy, and retain the original packaging and CoA for reference.
• Shipping: Temperature-sensitive products are shipped on dry ice. Confirm shipping conditions and estimated delivery times with your sales representative, particularly for international orders.

Frequently asked questions

What is the biological function of GOLGA7?

GOLGA7 is a member of the golgin coiled-coil protein family localized to the Golgi apparatus. It plays roles in maintaining Golgi morphology, vesicular tethering, and facilitating membrane trafficking. It has also been implicated in protein palmitoylation by interacting with DHHC-type palmitoyltransferases at the Golgi membrane.

What expression system is used to produce Recombinant Human GOLGA7 Protein (rp372892)?

The specific expression system for rp372892 is detailed in the product datasheet and Certificate of Analysis. Common systems for recombinant golgin-family proteins include E. coli, insect cell (baculovirus), and mammalian expression platforms. Please consult the product datasheet or contact Aladdin Scientific technical support for confirmed lot-specific information.

What applications is Recombinant Human GOLGA7 Protein validated for?

Recombinant Human GOLGA7 Protein is suitable for research applications including protein–protein interaction studies (e.g., pull-down, co-IP), antibody validation (Western blot, ELISA), biochemical enzyme assays, and structural or biophysical characterization. Validated applications for rp372892 are specified in the product datasheet; researchers should confirm suitability for their specific assay format.

How should Recombinant Human GOLGA7 Protein be stored to maintain activity?

For short-term storage (up to 1 month), –20 °C is generally appropriate. For long-term storage, –80 °C is recommended. Avoid repeated freeze–thaw cycles by preparing single-use aliquots upon first thaw. Keep the protein on ice during handling and consult the product-specific datasheet for formulation-dependent storage guidance.

Can Recombinant Human GOLGA7 Protein be used as an antibody validation standard?

Yes, recombinant proteins such as rp372892 are widely used as antigen standards to validate the specificity and sensitivity of anti-GOLGA7 antibodies in Western blot, ELISA, and dot blot formats. Using a well-characterized recombinant protein control improves assay reliability and reproducibility.

How do I place an order for rp372892 and what documentation is available?

Recombinant Human GOLGA7 Protein (rp372892) can be ordered directly through the Aladdin Scientific online store or via your institutional procurement system. A product datasheet, Safety Data Sheet (SDS), and lot-specific Certificate of Analysis (CoA) are available on the product page or upon request from customer service.

References

1. Munro S. The golgin coiled-coil proteins of the Golgi apparatus. Cold Spring Harb Perspect Biol. 2011;3(6):a005256. doi:10.1101/cshperspect.a005256
2. Fukata M, Fukata Y, Adesnik H, Bhaskara RM, et al. Identification of DHHC protein family members responsible for palmitoylation at the Golgi membrane. Mol Biol Cell. 2004.
3. Aladdin Scientific product page: Recombinant Human GOLGA7 Protein, SKU rp372892.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.