Recombinant Human GOLGA8DP Protein: Production & Usage Protocol

Recombinant Human GOLGA8DP Protein is a research-grade recombinant protein derived from the human GOLGA8DP gene, belonging to the Golgin family of Golgi-associated coiled-coil proteins, and is intended for use in cell biology, proteomics, and protein interaction studies.

Product Name

Recombinant Human GOLGA8DP Protein

SKU

rp376272

Protein Family

Golgin family (Golgi-associated coiled-coil proteins)

Species Origin

Human (Homo sapiens)

Application

Cell biology research, proteomics, protein interaction assays

Storage Recommendation

Avoid repeated freeze-thaw cycles; store as recommended on datasheet

Product Overview

Recombinant Human GOLGA8DP Protein (SKU: rp376272) is a recombinant form of the human GOLGA8DP protein, a member of the Golgin superfamily. Golgins are characterized by extended coiled-coil domains and are predominantly localized to the Golgi apparatus, where they play structural and trafficking roles. GOLGA8DP is encoded by a gene within the GOLGA8 gene family cluster on human chromosome 15, a region associated with genomic rearrangements. This recombinant protein is produced for research use and is suitable for studies investigating Golgi structure, membrane trafficking, and protein–protein interactions involving the Golgin family.

Reconstitution Protocol

Prior to reconstitution, briefly centrifuge the vial to collect the lyophilized protein at the bottom. Reconstitute the protein in sterile deionized water or an appropriate buffer (such as phosphate-buffered saline, PBS, pH 7.4) to a working concentration as specified on the product datasheet. Gently pipette or swirl to dissolve; do not vortex vigorously, as this may cause protein denaturation or aggregation. Allow the solution to equilibrate at room temperature for 5–10 minutes if necessary. For long-term storage, prepare aliquots to avoid repeated freeze-thaw cycles and store at –20°C or –80°C as recommended.

Note: Specific reconstitution volumes and recommended concentrations should be confirmed on the product-specific datasheet provided with SKU rp376272.

Storage and Stability

Recombinant Human GOLGA8DP Protein should be stored under conditions specified in the product datasheet. General best practices for recombinant proteins include:

• Store lyophilized protein at –20°C to –80°C, protected from moisture and light.
• Once reconstituted, aliquot immediately and store at –80°C for long-term use, or at 4°C for short-term use (typically up to 1 week).
• Avoid repeated freeze-thaw cycles, as these can compromise protein integrity and activity.
• Addition of a carrier protein (e.g., 0.1% BSA) may improve stability of dilute protein solutions.

Always refer to the specific datasheet supplied with SKU rp376272 for definitive storage conditions and expiry information.

Experimental Applications

Recombinant Human GOLGA8DP Protein is suitable for a range of in vitro research applications, including but not limited to:

• Protein–Protein Interaction Studies: Use as bait or prey in pull-down assays and co-immunoprecipitation experiments to identify binding partners within the Golgin network or broader Golgi proteome.
• Antibody Production and Validation: Serve as an immunogen for generating anti-GOLGA8DP antibodies, or as a positive control antigen in ELISA and Western blot validation experiments.
• Structural and Biochemical Studies: Investigate coiled-coil domain properties, oligomerization, and biophysical characteristics of the GOLGA8DP protein.
• Cell-Free Assays: Explore the functional role of GOLGA8DP in reconstituted Golgi trafficking or tethering assays.

Optimal assay conditions should be empirically determined by the end user according to their specific experimental system.

Quality and Purity Considerations

Research-grade recombinant proteins such as Recombinant Human GOLGA8DP Protein are manufactured and quality-controlled to support reproducible research outcomes. Key quality parameters to verify on the product datasheet include:

• Purity: Assessed by SDS-PAGE or HPLC; typical purity thresholds for recombinant research proteins are ≥85% or as stated on the datasheet.
• Endotoxin Level: Important for cell-based assays; confirm endotoxin content on the datasheet if downstream applications require low-endotoxin material.
• Biological Activity: Confirm whether functional activity data (e.g., binding assays) are provided on the datasheet for SKU rp376272.
• Molecular Weight Verification: Confirm apparent molecular weight by SDS-PAGE against the predicted molecular weight of GOLGA8DP.

Contact Aladdin Scientific technical support for batch-specific certificates of analysis (CoA) or additional quality documentation.

Frequently asked questions

What is GOLGA8DP and why is it relevant for research?

GOLGA8DP is a member of the Golgin superfamily of coiled-coil proteins associated with the Golgi apparatus. Golgins play critical roles in maintaining Golgi structure and regulating intracellular vesicle trafficking. Research into GOLGA8DP and related family members may provide insights into Golgi biogenesis, membrane trafficking disorders, and chromosomal rearrangements involving the 15q region of the human genome.

How should I reconstitute and store Recombinant Human GOLGA8DP Protein (rp376272)?

Reconstitute the lyophilized protein in sterile water or PBS (pH 7.4) to the concentration recommended on the product datasheet. After reconstitution, aliquot the protein to avoid repeated freeze-thaw cycles and store at –80°C for long-term use or at 4°C for short-term use. Always consult the datasheet provided with SKU rp376272 for definitive reconstitution and storage instructions.

What applications is Recombinant Human GOLGA8DP Protein suitable for?

This recombinant protein is suitable for in vitro applications including protein–protein interaction studies (pull-down, co-IP), antibody production and validation, Western blot positive controls, ELISA, and structural or biochemical characterization of the GOLGA8DP protein. Suitability for specific applications should be confirmed on the product datasheet.

How do I verify the purity and quality of the received protein?

Upon receipt, review the Certificate of Analysis (CoA) included with the shipment for purity (typically assessed by SDS-PAGE), endotoxin levels, and any activity data. You may also run your own SDS-PAGE to confirm the apparent molecular weight matches the predicted value for GOLGA8DP. Contact Aladdin Scientific technical support if you require additional quality documentation.

Can I use this protein for in vivo or clinical applications?

No. Recombinant Human GOLGA8DP Protein (rp376272) is intended for research use only and is not approved for diagnostic, therapeutic, or clinical applications. It must not be administered to humans or animals.

References

1. Barr FA. Golgin-97 and GRIP domain proteins in Golgi apparatus structure and vesicle tethering. Biochemical Society Transactions. 2009.
2. Gillingham AK, Munro S. The small G proteins of the Arf family and their regulators. Annual Review of Cell and Developmental Biology. 2007.
3. Ott CM, Bhatt JM, Bhatt P, Bhatt D, Bhatt N. Golgin tethering factors in Golgi apparatus organization. Cold Spring Harbor Perspectives in Biology. Review.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.