Recombinant Human LCLAT1 Protein: FAQs & Key Facts

Recombinant Human LCLAT1 Protein is a research-grade enzyme involved in cardiolipin and lysophospholipid remodeling, supplied by Aladdin Scientific to support studies in mitochondrial biology and lipid metabolism.

Product Name

Recombinant Human LCLAT1 Protein

SKU

rp369201

Protein Full Name

Lysocardiolipin Acyltransferase 1 (LCLAT1)

Species

Human (Homo sapiens)

Protein Type

Recombinant enzyme (acyltransferase)

Primary Research Area

Lipid metabolism, mitochondrial biology, cardiolipin remodeling

What Is LCLAT1 and Why Is It Important in Research?

LCLAT1 (Lysocardiolipin Acyltransferase 1), also known as AGPAT8, is a membrane-associated enzyme that catalyzes the acylation of lysocardiolipin (MLCL) to cardiolipin (CL), a critical phospholipid predominantly found in the inner mitochondrial membrane. Cardiolipin plays essential roles in maintaining mitochondrial membrane integrity, supporting the electron transport chain, regulating apoptosis, and facilitating mitophagy. LCLAT1 also possesses lysophosphatidylinositol acyltransferase activity, contributing to broader phospholipid remodeling pathways. Because cardiolipin dysregulation is implicated in a range of diseases—including Barth syndrome, heart failure, and neurodegeneration—LCLAT1 is an important target for researchers studying mitochondrial dysfunction and lipid-related pathologies.

Product Specifications and Quality Assurance

Aladdin Scientific's Recombinant Human LCLAT1 Protein (SKU: rp369201) is produced as a recombinant protein for use in biochemical and cell biology research. Researchers should consult the product datasheet for confirmed details regarding the expression system (e.g., E. coli, baculovirus, or mammalian), tag configuration, molecular weight, purity grade, and formulation buffer, as these parameters are critical for assay design. Quality control testing typically includes SDS-PAGE analysis and functional verification where applicable. All lot-specific certificates of analysis (CoA) are available upon request and can be accessed through your Aladdin Scientific account portal.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling of Recombinant Human LCLAT1 Protein are essential to maintain activity and prevent degradation. As a general best practice for recombinant proteins: store lyophilized material at –20 °C or –80 °C as indicated on the product datasheet; avoid repeated freeze-thaw cycles by preparing single-use aliquots after reconstitution; reconstitute in the buffer specified in the datasheet (typically sterile water or a carrier protein-containing buffer such as 0.1% BSA in PBS); and keep reconstituted protein on ice during active use. For long-term storage of reconstituted protein, addition of a carrier protein and storage at –80 °C is recommended. Always consult the specific product datasheet for authoritative storage and reconstitution instructions, as conditions may vary by lot and formulation.

Research Applications for Recombinant Human LCLAT1 Protein

Recombinant Human LCLAT1 Protein is suited for a range of in vitro research applications, including: Enzymatic activity assays — measuring acyltransferase activity toward lysocardiolipin or lysophospholipid substrates; Protein interaction studies — identifying binding partners through pull-down or co-immunoprecipitation experiments; Structural biology — supporting crystallography or cryo-EM efforts when sufficient quantities and purity are achieved; Drug discovery and screening — serving as a target enzyme in inhibitor or activator screening campaigns relevant to mitochondrial disease or metabolic disorders; and Antibody validation — acting as a positive control antigen for Western blot or ELISA-based antibody characterization. Researchers should validate the protein in their specific assay system and consult the datasheet for confirmed application suitability.

Ordering, Documentation, and Customer Support

Recombinant Human LCLAT1 Protein (SKU: rp369201) can be ordered directly through the Aladdin Scientific website or by contacting your regional sales representative. Bulk quantities, custom formulations, or specific endotoxin-tested preparations may be available upon request—please contact our technical sales team for a quote. All orders are accompanied by a product datasheet; certificates of analysis (CoA) and safety data sheets (SDS) are available via your online account or upon request. For technical questions regarding experimental design, protocol optimization, or product compatibility, Aladdin Scientific's technical support team is available by email or phone. Standard shipping conditions for recombinant proteins involve dry ice to maintain cold-chain integrity; please review our shipping and returns policy for further details.

Frequently asked questions

What is the biological function of LCLAT1?

LCLAT1 (Lysocardiolipin Acyltransferase 1) catalyzes the reacylation of lysocardiolipin to mature cardiolipin in the inner mitochondrial membrane, a key step in cardiolipin remodeling. It also exhibits lysophosphatidylinositol acyltransferase activity. These functions make it central to mitochondrial membrane integrity, apoptosis regulation, and energy metabolism research.

What expression system is used to produce Recombinant Human LCLAT1 Protein (rp369201)?

The specific expression system (e.g., E. coli, baculovirus-insect cell, or mammalian cell) for SKU rp369201 is detailed in the product datasheet. Please refer to the datasheet or contact Aladdin Scientific technical support for confirmed information, as this detail is critical for downstream application compatibility.

How should Recombinant Human LCLAT1 Protein be stored to maintain activity?

For best results, store lyophilized protein at –20 °C or –80 °C as directed by the product datasheet, and avoid repeated freeze-thaw cycles by aliquoting upon reconstitution. Reconstituted protein should be stored at –80 °C with an appropriate carrier protein such as 0.1% BSA for long-term stability. Always refer to the specific product datasheet for authoritative storage instructions.

What research applications is Recombinant Human LCLAT1 Protein suitable for?

Recombinant Human LCLAT1 Protein is suitable for in vitro enzymatic activity assays, protein-protein interaction studies, antibody validation (e.g., as a Western blot positive control), and drug discovery screening targeting mitochondrial lipid pathways. Suitability for specific applications should be confirmed with the product datasheet or Aladdin Scientific technical support.

How can I obtain a Certificate of Analysis (CoA) for my lot of LCLAT1 protein?

Lot-specific Certificates of Analysis (CoA) for Recombinant Human LCLAT1 Protein (rp369201) are available through your Aladdin Scientific online account portal. You may also request a CoA by contacting Aladdin Scientific customer service with your order number and lot number. Safety Data Sheets (SDS) are similarly accessible through the same channels.

References

1. Houtkooper RH, Vaz FM. Cardiolipin, the heart of mitochondrial metabolism. Cell Mol Life Sci. 2008;65(16):2493–2506. doi:10.1007/s00018-008-8030-5
2. Zhao Y, et al. Identification and characterization of a major liver lysophosphatidylcholine acyltransferase. J Biol Chem. 2008;283(13):8258–8265.
3. Aladdin Scientific Product Datasheet: Recombinant Human LCLAT1 Protein, SKU rp369201. Aladdin Scientific (absea #PP-3736).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.