Recombinant Human LYPLA2 Protein: Key Facts & FAQs

Recombinant Human LYPLA2 Protein is a research-grade enzyme reagent representing the human acyl-protein thioesterase 2 (APT2), produced for use in biochemical assays, enzyme activity studies, and protein depalmitoylation research.

Product Name

Recombinant Human LYPLA2 Protein

SKU

rp370439

Protein Function

Acyl-protein thioesterase 2 (APT2); catalyzes removal of palmitoyl groups from S-acylated proteins

Protein Type

Recombinant human protein

Primary Application

Enzyme activity assays, depalmitoylation studies, biochemical and cell biology research

Storage Recommendation

Refer to product datasheet; typically stored at -20°C or -80°C; avoid repeated freeze-thaw cycles

What Is Recombinant Human LYPLA2 Protein?

LYPLA2 (Lysophospholipase 2), also known as Acyl-Protein Thioesterase 2 (APT2), is a serine hydrolase enzyme encoded by the human LYPLA2 gene. It belongs to the metabolic serine hydrolase superfamily and plays a central role in the dynamic regulation of protein S-palmitoylation — a reversible lipid modification critical to membrane association, protein trafficking, and signal transduction.

Recombinant Human LYPLA2 Protein (SKU: rp370439), available from Aladdin Scientific, is a recombinant form of this enzyme produced for research applications. It enables scientists to study depalmitoylation mechanisms, screen for APT2 inhibitors, and investigate the biology of lipid-modified proteins in vitro.

Biological Role and Research Significance of LYPLA2

LYPLA2/APT2 functions as a cytosolic thioesterase that cleaves thioester bonds between fatty acids (primarily palmitate) and cysteine residues on substrate proteins. Together with its paralog APT1 (LYPLA1), APT2 constitutes a major depalmitoylating enzyme pair responsible for cycling palmitoyl groups on and off proteins at cellular membranes.

Key substrate proteins regulated by LYPLA2-mediated depalmitoylation include members of the Ras GTPase family, heterotrimeric G-protein alpha subunits, and other peripherally membrane-associated signaling proteins. Dysregulation of this enzyme has been implicated in oncogenic signaling, making LYPLA2 a potential therapeutic target in cancer biology research.

Research using recombinant LYPLA2 protein supports studies in: protein lipidation and membrane dynamics; RAS pathway biology; drug discovery (APT2 inhibitor screening); and chemical biology investigations of S-acylation cycles.

Recommended Applications and Usage Guidelines

Recombinant Human LYPLA2 Protein is suitable for a range of in vitro research applications, including but not limited to:

• Enzyme Activity Assays: Measuring thioesterase activity using fluorogenic or colorimetric substrates to characterize LYPLA2 kinetics.
• Inhibitor Screening: Identifying and validating small-molecule inhibitors of APT2 for chemical biology or drug discovery programs.
• Substrate Profiling: Investigating the substrate specificity and depalmitoylation efficiency of LYPLA2 against acylated peptide or protein substrates.
• Biochemical Interaction Studies: Exploring protein–protein interactions involving LYPLA2 using pull-down or co-immunoprecipitation formats adapted for recombinant protein use.

Always consult the product datasheet for the recommended working concentration, buffer conditions, and assay formats specific to this lot. Reconstitute and handle the protein according to the datasheet to preserve enzymatic activity.

Storage, Stability, and Handling

Proper storage and handling of Recombinant Human LYPLA2 Protein are essential to maintain its enzymatic activity and experimental reproducibility. The following general guidelines apply to most recombinant enzyme preparations; always refer to the specific product datasheet (SKU: rp370439) for lot-specific instructions:

• Temperature: Store at -20°C for short-term storage or -80°C for long-term storage.
• Freeze-Thaw Cycles: Minimize repeated freeze-thaw cycles. Aliquot upon first thaw to preserve activity.
• Carrier Protein: Many recombinant proteins are supplied with BSA or glycerol as stabilizers; check the datasheet for formulation details.
• Sterility: Handle under aseptic conditions if the protein will be used in cell-based assays.

Improper storage can result in protein aggregation, loss of enzymatic activity, or degradation. Contact Aladdin Scientific technical support if you have concerns about product quality upon receipt.

Ordering and Technical Support

Recombinant Human LYPLA2 Protein is available from Aladdin Scientific under SKU rp370439. To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative.

For technical questions regarding experimental design, assay optimization, or protein handling, please reach out to Aladdin Scientific's technical support team. Provide your SKU, lot number, and a description of your application for the most efficient assistance.

Bulk quantities, custom formulations, or specific quality documentation (e.g., CoA, SDS) may be available upon request. Please inquire with the sales team for custom project requirements.

Frequently asked questions

What is the biological function of LYPLA2?

LYPLA2 (also called Acyl-Protein Thioesterase 2, APT2) is a serine hydrolase that removes palmitoyl groups from S-acylated proteins, thereby regulating their membrane association and signaling activity. It plays a key role in the palmitoylation–depalmitoylation cycle of proteins such as Ras GTPases and G-protein alpha subunits.

What applications is Recombinant Human LYPLA2 Protein suitable for?

Recombinant Human LYPLA2 Protein is suitable for in vitro enzyme activity assays, APT2 inhibitor screening, substrate specificity studies, and biochemical interaction experiments. It is designed for research use only and is not intended for therapeutic or diagnostic applications.

How should Recombinant Human LYPLA2 Protein be stored to maintain activity?

The protein should generally be stored at -20°C for short-term use or -80°C for long-term storage. Repeated freeze-thaw cycles should be avoided by aliquoting the protein upon first thaw. Always refer to the specific product datasheet for lot-specific storage instructions.

What is the catalog number (SKU) for Recombinant Human LYPLA2 Protein from Aladdin Scientific?

The SKU for Recombinant Human LYPLA2 Protein from Aladdin Scientific is rp370439. You can order this product directly through the Aladdin Scientific website or by contacting your regional sales representative.

Is Recombinant Human LYPLA2 Protein suitable for use in cell-based assays?

Recombinant Human LYPLA2 Protein can be used in cell-based experimental contexts where exogenous enzyme addition is appropriate; however, it should be handled under aseptic conditions and the formulation should be checked for components compatible with cell culture. Consult the product datasheet and Aladdin Scientific technical support for guidance on cell-based applications.

References

1. Rocks O, et al. An acylation cycle regulates localization and activity of palmitoylated Ras isoforms. Science. 2005;307(5716):1746-1752. doi:10.1126/science.1105654
2. Abrami L, et al. Palmitoylated acyl protein thioesterases APT1 and APT2 depalmitoylate the spike glycoprotein of SARS-CoV-2. Nature Chemical Biology. 2021.
3. Aladdin Scientific. Recombinant Human LYPLA2 Protein, SKU rp370439. Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.