Recombinant Human MCF2L Protein: FAQs & Key Facts

Recombinant Human MCF2L Protein is a research-grade guanine nucleotide exchange factor (GEF) that activates Rho-family GTPases and plays critical roles in cytoskeletal organization, cell migration, and intracellular signaling cascades.

Product Name

Recombinant Human MCF2L Protein

SKU

rp371619

Protein Family

Dbl family Rho guanine nucleotide exchange factors (RhoGEFs)

Gene Name

MCF2L (also known as DBS)

Primary Function

Acts as a GEF for Rho-family GTPases, promoting GDP-to-GTP exchange to regulate cytoskeletal dynamics and cell signaling

Recommended Storage

Store at -80 °C; avoid repeated freeze-thaw cycles (specific conditions confirmed on product datasheet)

What Is MCF2L and Why Is It Important in Research?

MCF2L (also designated DBS) is a member of the Dbl family of Rho guanine nucleotide exchange factors (RhoGEFs). It catalyzes the exchange of GDP for GTP on Rho-family small GTPases, including RhoA and Cdc42, thereby acting as an upstream activator of these pivotal signaling molecules. Activated Rho GTPases regulate a wide range of cellular processes including actin cytoskeleton remodeling, cell polarity, cell migration, and proliferation. Research interest in MCF2L has grown substantially due to its involvement in neuronal development, oncogenesis, and cardiovascular biology. Understanding MCF2L activity is therefore relevant to studies in cancer biology, neuroscience, and developmental biology.

What Are the Recommended Applications for Recombinant Human MCF2L Protein?

Recombinant Human MCF2L Protein (rp371619) is designed to support a variety of in vitro research applications. Typical uses include:

• Biochemical assays: GEF activity assays to measure nucleotide exchange kinetics on Rho GTPase substrates.
• Protein–protein interaction studies: Pull-down assays and co-immunoprecipitation experiments to map MCF2L binding partners.
• Structural studies: As a component for crystallography or biophysical characterization of GEF–GTPase complexes.
• Cell-based research: Exogenous addition or use as a reference standard in signaling pathway investigations.
• Antibody validation: As a positive control antigen in Western blotting or ELISA development.

Please consult the product datasheet for confirmed, validated application data specific to lot rp371619.

How Should Recombinant Human MCF2L Protein Be Stored and Handled?

Proper storage and handling are essential to maintain the activity and integrity of recombinant proteins. General best-practice guidelines for Recombinant Human MCF2L Protein include:

• Long-term storage: Store at -80 °C immediately upon receipt.
• Short-term storage: If repeated access is needed within days, aliquot the protein into single-use volumes before freezing to avoid repeated freeze-thaw cycles, which can denature the protein and reduce activity.
• Thawing: Thaw on ice or at 4 °C; avoid thawing at room temperature.
• Avoid: Vortexing or vigorous agitation; use gentle mixing instead.
• Working solutions: Prepare fresh dilutions in appropriate buffer immediately before use.

Note: Always refer to the specific lot datasheet supplied with your product for confirmed storage buffer composition, concentration, and recommended working conditions.

Quality, Purity, and Production of Recombinant Human MCF2L Protein

Recombinant Human MCF2L Protein offered by Aladdin Scientific is produced using recombinant expression systems. Research-grade recombinant proteins such as this are typically characterized by:

• Expression system: Commonly produced in mammalian, baculovirus/insect, or E. coli expression systems depending on the requirements for post-translational modifications and folding (refer to datasheet for the specific system used for rp371619).
• Purity: High-purity preparations are verified by SDS-PAGE and/or HPLC analysis.
• Endotoxin testing: Endotoxin levels are typically controlled and measured by LAL assay for research applications requiring low endotoxin.
• Functional validation: Activity may be confirmed by in vitro GEF assay or comparable functional test.

For exact specifications including purity percentage, host expression system, tag information, and endotoxin levels, please consult the official product datasheet for SKU rp371619 on the Aladdin Scientific website.

How to Order Recombinant Human MCF2L Protein from Aladdin Scientific

Ordering Recombinant Human MCF2L Protein (SKU: rp371619) from Aladdin Scientific is straightforward:

1. Online ordering: Search for SKU rp371619 on the Aladdin Scientific website and add to your cart. Institutional purchase orders and credit card payments are accepted.
2. Quotation requests: For bulk quantities, custom formulations, or institutional pricing, contact Aladdin Scientific's sales team directly for a formal quotation.
3. Shipping: Recombinant proteins are shipped on dry ice to maintain cold-chain integrity. Confirm delivery schedules with your regional Aladdin representative.
4. Returns and replacements: If the product does not meet stated specifications upon receipt, contact customer service within the stated returns window as described in Aladdin's returns policy.

For further assistance, reach out to Aladdin Scientific customer support or your local distributor.

Frequently asked questions

What is the biological function of MCF2L protein?

MCF2L is a Rho guanine nucleotide exchange factor (RhoGEF) that promotes the activation of Rho-family GTPases such as RhoA and Cdc42 by catalyzing the exchange of GDP for GTP. This activity regulates downstream pathways controlling actin cytoskeleton dynamics, cell migration, and cell proliferation. Its activity is relevant to neuronal development, cancer biology, and cardiovascular research.

What expression system is used to produce Recombinant Human MCF2L Protein (rp371619)?

The specific expression system (e.g., mammalian, baculovirus/insect, or E. coli) used for rp371619 is detailed in the product datasheet. Please download the datasheet from the Aladdin Scientific product page or contact customer support for this information, as it is critical for assessing post-translational modifications relevant to your assay.

How should I store Recombinant Human MCF2L Protein to preserve activity?

For long-term preservation, store Recombinant Human MCF2L Protein at -80 °C immediately upon receipt. Prior to freezing, aliquot the protein into single-use volumes to prevent activity loss from repeated freeze-thaw cycles. When thawing, do so gently on ice or at 4 °C, and avoid vigorous vortexing. Specific buffer composition and storage recommendations are confirmed in the lot-specific datasheet.

Can Recombinant Human MCF2L Protein be used as a positive control in Western blotting?

Yes, recombinant proteins are commonly used as positive control antigens in Western blotting to validate antibody specificity and confirm expected molecular weight bands. Recombinant Human MCF2L Protein (rp371619) can serve this purpose; however, please verify compatibility with your specific antibody and assay conditions by consulting the datasheet or contacting Aladdin Scientific technical support.

How do I request a quote or place an order for Recombinant Human MCF2L Protein?

You can order Recombinant Human MCF2L Protein directly on the Aladdin Scientific website by searching for SKU rp371619 and proceeding through the standard checkout. For bulk orders, institutional pricing, or custom requirements, contact the Aladdin Scientific sales team to request a formal quotation. The product is shipped on dry ice to maintain cold-chain integrity during transit.

References

1. Bhavna Bhatt, Dbl Family of Guanine Nucleotide Exchange Factors: A Review of their Structure, Function and Regulation. Textbook-level background on RhoGEF biology.
2. Aladdin Scientific. Recombinant Human MCF2L Protein, SKU rp371619. Product Datasheet.
3. Rossman KL, Der CJ, Sondek J. GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors. Nat Rev Mol Cell Biol. 2005;6(2):167-180. doi:10.1038/nrm1587

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.