Sirtuins are a family of NAD⁺-dependent deacylases comprising SIRT1–7 and are involved in metabolism, DNA repair, mitochondrial homeostasis, and inflammatory regulation. Animal studies suggest that certain members may improve healthspan, but there is currently no direct evidence that they ...
Topic
Aging
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
4 resources · protocols, FAQs & articles
Dietary restriction, Rapamycin, α-ketoglutarate (AKG), and Carglumic Acid influence aging-related processes through nutrient availability, mTOR signaling, central metabolism, and nitrogen metabolism, respectively. Their effects involve autophagy, proteostasis, mitochondrial function, and ...
Aging involves persistent dysregulation of nutrient sensing, energy metabolism, protein homeostasis, and chronic inflammation. IIS, mTOR, AMPK, NF-κB, and Sirtuins are interconnected through nodes including AKT, FOXO, ULK1, and NAD⁺ and jointly regulate cell growth, autophagy, stress ...
NAD (nicotinamide adenine dinucleotide, Nicotinamide Adenine Dinucleotide), also known as Coenzyme I, is composed of nicotinamide, adenine, and two ribose units linked via a phosphodiester bond. It forms a reversible redox pair as oxidized NAD⁺ and reduced NADH, serving as an electron carrier ...
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Techniques often explored alongside immunological experiments.