T-cell engagers (TCEs) are immunotherapeutic molecules that simultaneously recognize T cells and specific target cells, thereby inducing directed T-cell cytotoxicity. In cancer research, a classical TCE binds a tumor-cell surface antigen through one arm and T-cell CD3 through the other. This ...
Topic
Cytotoxicity
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
4 resources · protocols, FAQs & articles
Differences in cytotoxicity evaluation arise first from differences in the biological process being measured. The Neutral Red assay primarily reflects cellular uptake of the dye and lysosomal retention capacity, whereas the MTT assay and the CCK-8 assay mainly reflect dehydrogenase-related ...
CCK-8 (Cell Counting Kit-8) is a colorimetric cell-viability assay system based on the water-soluble tetrazolium salt WST-8. It is widely used for cell proliferation, cytotoxicity, and drug-sensitivity evaluation.
Polyethyleneimine (PEI) is a widely used non-viral vector for gene transfection. Its cationic nature enables it to effectively bind DNA and facilitate its entry into cells.
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Techniques often explored alongside immunological experiments.