Immunohistochemistry

Step-by-step protocol for PSMD7 Mouse mAb (Ab349226): sample prep, WB, IHC, and IP workflows for reliable 26S proteasome research.

Step-by-step protocol for PPP3R1 Mouse mAb (Ab349215): sample prep, recommended dilutions, WB/IHC/IF applications, and troubleshooting tips.

Learn how to select, validate, and apply Recombinant PUS7L Antibody (Ab349233) in WB, IHC, and IF assays with expert protocols from Aladdin Scientific.

Step-by-step guidelines for selecting, validating, and using Recombinant RAB6B Antibody (Ab349238) in WB, IHC, and IF applications.

Step-by-step protocol for RAGE Mouse mAb (Ab349240): sample prep, antibody dilution, incubation, and detection for WB, IHC, and IF assays.

Step-by-step guidance on selecting, validating, and using PELI3 Mouse mAb (Ab350624) for immunoassays. Covers WB, IHC, and storage best practices.

Step-by-step protocol for NUDT5 Mouse mAb (Ab350593): recommended dilutions, storage, WB, IHC, and IF applications for reliable results.

Step-by-step protocol for RAPGEF5 Mouse mAb (Ab350674): recommended dilutions, storage, WB, IHC, and IF applications for research use.

Comprehensive guidelines for using PSMA3 Mouse mAb (Ab349223) in immunological assays—covering target biology, applications, protocols, and storage tips.

Step-by-step protocol for OPALIN Antibody (Ab351131): sample prep, blocking, incubation, and detection for WB, IHC, and ICC applications.

Step-by-step protocol for ADAM9 Mouse mAb (Ab351140): optimal dilutions, sample prep, storage, and troubleshooting for WB, IHC, and IF applications.

Step-by-step protocol for PF4V1 Mouse mAb (Ab351342): storage, reconstitution, WB, IHC, and ELISA applications for research labs.

Step-by-step protocol for PREPL Mouse mAb (Ab350650): optimal dilutions, storage, and application tips for WB, IHC, and IF assays.

Step-by-step guidelines for using RBM3 Mouse mAb (Ab349618) in WB, IHC, and IF assays. Optimize your cold-shock protein detection experiments.

Step-by-step protocol for WDR70 Mouse mAb (Ab349685): sample prep, optimal dilutions, and detection for WB, IHC, and IP applications.

A practical guide to selecting, validating, and applying COA4 Antibody (Ab348860) in your research workflows, covering WB, IHC, and IF applications.

Comprehensive guideline for SOX8 Mouse mAb (Ab349648): applications, recommended protocols, storage, and troubleshooting for optimal IHC, WB, and IF results.

Step-by-step protocol for c-Jun Mouse mAb (Ab350278): sample prep, dilutions, and detection for Western blot, IHC, and immunoprecipitation.

Step-by-step protocol for RASSF2 Mouse mAb (Ab351050): sample prep, dilutions, and detection for WB, IHC, and IP applications.

Explore PDLIM5 Mouse mAb (Ab349189) for WB, IHC and IP. Learn target biology, validated applications, and optimized protocols for your research workflow.

Learn how APES Adhesion Promoter improves tissue section adhesion on glass slides for IHC and histology. Storage, usage, and key benefits explained.

Step-by-step IHC staining protocol for Ready-to-Use SABC Kit (Mouse). Covers tissue prep, blocking, antibody incubation, and DAB detection.

This procedure is intended to standardize tissue pretreatment, fixation, sucrose cryoprotection, OCT embedding, rapid freezing, cryosectioning, section collection, frozen storage, and thawing procedures. It is applicable to immunofluorescence, immunohistochemistry, enzyme histochemistry, lipid ...

Identification of hepatitis B virus (HBV) in liver tissue should not rely on a single staining result. HE staining can indicate the pathological background of chronic hepatitis, interface hepatitis, hepatocyte degeneration, fibrosis, and “ground-glass hepatocytes,” but it cannot directly ...

TSA, or Tyramide Signal Amplification, is a signal amplification technique based on HRP (Horseradish Peroxidase)-catalyzed reactions. It is commonly used in IHC (Immunohistochemistry), ICC (Immunocytochemistry), ISH (In Situ Hybridization), FISH (Fluorescence In Situ Hybridization), and ...

Fixation is a critical pretreatment step in histology, cytology, immunostaining, and ultrastructural observation. Its core purpose is to preserve the in situ morphology, molecular localization, and structural stability of samples as much as possible. Glutaraldehyde, paraformaldehyde, and ...

Mounting is not merely an ancillary step after staining, but a critical determinant of section transparency, fluorescence stability, background control, and storage life. In experiments such as immunofluorescence, immunohistochemistry, in situ hybridization, and lipid staining, different ...

Immunohistochemistry Protocol

Analysis and handling of IHC common problems

Immunohistochemistry (IHC) is a technique that uses the principle of specific binding between antigens and antibodies to locate, characterize and quantify antigens (peptides and proteins) within tissue cells by chemically reacting the marker antibody's color developer (fluorescein, enzyme, metal ...

Steps of paraffin section immunohistochemistry

Immunohistochemistry kit experimental procedure

Choosing monoclonal or polyclonal antibodies? A single specific antibody produced by one type of B cell is called a monoclonal antibody. Monoclonal antibodies bind to a single specific antigenic determinant in a targeted manner, like a missile hitting a target with precision.

Most formalin-fixed tissues require an antigen retrieval step before immunohistochemical staining. Methylene bridges formed during fixation cross-link proteins and mask antigenic sites. Antigen retrieval methods break these methylene bridges and expose antigenic sites, allowing antibodies to ...

Immunohistochemical staining protocol for frozen samples (IHC-Fr)