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Tissue Homogenization Buffer for ELISA

Tissue Homogenization Buffer for ELISA

posted this at Jun 9, 2025 Updated at Jul 27, 2023

Recipe

Stock Solution:

1.Weigh out 0.025 grams of Aprotinin and Luepeptin and dissolve in 25ml of water

2.Mix and aliquot into 70ul single use aliquots

To prepare homogenization buffer:

1.Add 50ul of stock solution to 100ml of PBS

2.Sterilze by filtration

Final concentration is 0.5ug/ml

Categories: Protocols

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