GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)See COA
Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA Azide Free,Carrier Free,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti GFP Antibody, Monoclonal antibody(F56-6A1.2.3), could be used for WB, ICC, IF and so on.
Specifications
Product Name
GFP Mouse mAb - Primary antibody, specific to GFP, IgG2b
Synonyms
GFP antibody | Green fluorescent protein antibody | eGFP | Enhanced Green Fluorescent Protein | GFP | GFPuv | green fluorescent protein (gfp) | Green Fluorescent Protein | GFP Tag antibody | Anti- GFP Tag antibody
Specifications & Purity
Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca 2+ -activated photoprotein aequorin. Subunit struct
Host species
Mouse
Specificity
GFP
Immunogen
Full length enhanced GFP.
Positive Control
HEK293 cells transfected with GFP tag.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, PBS Only, Validated
Isotype
IgG2b
SDS-PAGE
26.4 kDa (Light Chain) & 50.3 kDa (Heavy Chain), under reducing conditions; 191.1 kDa, under non-reducing conditions.
Purification Method
Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.
Images
GFP Mouse mAb (Ab105280) - Western Blot All lanes: GFP Mouse mAb (Ab105280) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2.8 s
Application
Application
Dilution info
WB
Use at an assay dependent concentration.
IF/ICC
Use at an assay dependent concentration.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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