Golgi-Tracker Red - ≥97% , CAS No.571186-05-5

CAS: 571186-05-5 Cat. No.: G746380 Formula: C₃₉H₅₀BN₃O₄F₂S Molecular Weight: 705.71

GRADE & PURITY ≥97%

Synonyms

BDP TR ceramide | BODIPY TR Ceramide | BODIPY TR-Ceramide

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Overview

For trace amounts of liquid, centrifuge briefly before each use to collect the liquid at the bottom of the tube.Fluorescence intensity decays. Avoid light to minimize the quenching of fluorescence.Coverslips and slides are required but not supplied. They can be ordered from .Golgi-Tracker Red is suitable for staining Golgi in live cells, but not Golgi in fixed cells.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.Preparation of Golgi-Tracker Red working solutiona.Dilute an appropriate amount of Golgi-Tracker Red (33.3mg/ml) in the Golgi-Tracker Red Dilution Buffer at a ratio of 1:100. For example, mix 1µl of Golgi-Tracker Red with 1ml of Golgi-Tracker Red Dilution Buffer to obtain the Golgi-Tracker Red working solution.Note: The dilution ratio of Golgi-Tracker Red can be adjusted within the range of 1:50-1:200 based on the staining results. b.Golgi-Tracker Red working solution can be recovered and stored at 4℃ or -20℃ for future uses until it no longer works. Store at 4℃ if it will be reused within 1-2 days , or at -20℃ for longer period of storage.2.Fluorescent labeling of Golgi bodies in live cellsa.Remove the cell culture medium and wash cells grown on coverslips with an appropriate amount of solution such as Hanks' Balanced Salt Solution with Ca2+ & Mg2+ (, C0219). For staining of suspension cells, centrifuge to collect cells and refer to the staining method for adherent cells.b.Remove the wash buffer, add the Golgi-Tracker Red working solution and incubate at 4℃ for 30 min.c.Recover the Golgi-Tracker Red staining working solution, wash cells about 3 times with cell culture medium pre-cooled on ice, and incubate with fresh culture medium at 37℃ for 30 minutes.d.Wash again with fresh culture medium, followed by observation under fluorescence microscope or confocal microscope. Bright red fluorescent Golgi can be observed, while other membrane systems within cells show a fainter fluorescent staining.

Specifications

Synonyms

BDP TR ceramide | BODIPY TR Ceramide | BODIPY TR-Ceramide

Specifications & Purity

≥97%

Stability And Storage

Store at -20℃ long term (2 years). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥97%

Names and Identifiers

• Molecular Weight: 705.71

Chemical and Physical Properties

• Sensitivity: light sensitive