β-Mannanase, CAS No.37288-54-3

CAS: 37288-54-3 Cat. No.: G303580 EC Number: 253-446-5

GRADE & PURITY EnzymoPure™, Enzyme activity 50000u/g

Bioactivity

≥50000 U/g enzyme powder

Overview

Mannanase is a class of enzymes capable of hydrolyzing mannans. Mannan is a type of saccharide. When mannanase acts on mannan molecules, it hydrolyzes them into the monosaccharide mannose and oligosaccharides such as manno‑oligosaccharides. These hydrolysates feature high solubility, which helps improve the taste and processability of food products. By recognizing and cleaving β‑1,4‑glycosidic linkages within the mannan backbone, this enzyme breaks long‑chain polysaccharides down into manno‑oligosaccharides (e.g., mannobiose, mannotriose) or monosaccharides. Some β‑mannanases can work synergistically with other enzymes (e.g., cellulase, xylanase) to degrade plant cell walls and boost substrate‑degradation efficiency. 

Application Fields

1. Feed industry: Addition of β‑mannanase degrades antinutritional factors in grains (e.g., galactomannans in legumes), improves animal feed digestibility, and reduces accumulation of viscous substances in the intestine. 

2. Food processing: Applied in the production of coffee, fruit juice and other products to reduce viscosity and modify texture; it is also used for the preparation of functional oligosaccharides (prebiotics) to support intestinal health. 

3. Bioenergy: As one of the key enzymes for lignocellulose pretreatment, it facilitates the release of cellulose components and improves ethanol conversion efficiency. 

4. Paper‑making and textile industries: It assists in degrading gums contained in plant fibers, lowers the dosage of chemical bleaching agents, and supports eco‑friendly production processes.

Specifications

Product Name

β-Mannanase, CAS No.37288-54-3

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, Enzyme activity 50000u/g

Biochemical and Physiological Mechanisms

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Bioactivity

≥50000 U/g enzyme powder

CAS

37288-54-3

Enzyme Commission Number

EC 3.2.1.78

Molecule Type

Enzyme

Storage and Shipping

Concentration

Enzyme activity 50000u/g

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Unit definition

At a temperature of 37 ℃ and pH value of 5.5, 1 is released every minute by degradation from a mannan solution with a concentration of 3mg/mL μ The amount of enzyme required for reducing sugar in mol is one enzyme activity unit (U).

References

1. Wenxuan Jiang, Xiangrong Deng, Lanxian Qin, Dahai Jiang, Mengqi Lu, Kai Chen, Manqi Yang, Liangliang Zhang, Jianchun Jiang, Liming Lu.  (2024)  Research on the Cell Wall Breaking and Subcritical Extraction of Astaxanthin from Phaffia rhodozyma.  MOLECULES,  29  (17): (4201).  [PMID:39275049] [10.3390/molecules29174201]

2. Ma Guozhi, Wang Xuan, Cai Jihai, Wang Xiaoying.  (2026)  Mannan oligosaccharide-S. typhimurium interaction mediated fluorescent-recovery of carbon dots/molybdenum disulfide probe for rapid detection.  MICROCHIMICA ACTA,  193  (8): (550).  [PMID:42458147] [10.1007/s00604-026-08252-8]