Haptoglobin Mouse mAb - Primary antibody, specific to Haptoglobin, Mouse IgG2b

Cat. No.: Ab106882

GRADE & PURITY Carrier Free, ExactAb™, Validated, High Performance, See COA

Application

IHC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG2b

Clone Number

C12

Conjugation

Unconjugated

Overview

Mouse anti Human Haptoglobin Antibody, Monoclonal (C12), could be used for WB, IHC and so on.
Application:
WB: 0.01-5 µg/mL
IHC: 5-30 µg/mL
Protein Function:
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

Specifications

Product Name

Haptoglobin Mouse mAb - Primary antibody, specific to Haptoglobin, Mouse IgG2b

Synonyms

Zonulin | Binding peptide antibody | BP antibody | Haptoglobin alpha chain antibody | Haptoglobin alpha(1S) beta antibody | Haptoglobin alpha(2FS) beta antibody | Haptoglobin beta chain antibody | Haptoglobin, alpha polypeptide antibody | Haptoglobin, bet

Specifications & Purity

Carrier Free, ExactAb™, Validated, High Performance, See COA

Host species

Mouse

Specificity

Haptoglobin

Immunogen

Recombinant Hpt expressed in E.coli (AA 227-378).

Positive Control

WB: Mouse liver lysates. IHC: Human liver cancer tissue.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, High Performance, Validated

Isotype

Mouse IgG2b

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified + Protein G purified

Concentration

See COA

Product Line

biological_products

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Haptoglobin Mouse mAb (Ab106882)

Haptoglobin Mouse mAb (Ab106882) - Western Blot
All lanes: Haptoglobin Mouse mAb (Ab106882) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 20.0 s

IHC: Haptoglobin Mouse mAb (Ab106882)

Haptoglobin Mouse mAb (Ab106882) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 30 µg/mL Haptoglobin Mouse mAb (Ab106882)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Application

Application

Dilution info

IHC

5-30 µg/mL

WB

0.01-5 µg/mL

Associated Targets(Human)

HP Tbio Haptoglobin (0 Activities)

Discover Target: HP

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Frequently Asked Questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Haptoglobin Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.