Hsf1 Mouse Pre-designed siRNA Set A

Cat. No.: H1479929

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Hsf1 Mouse Pre-designed siRNA Set A contains three designed siRNAs for Hsf1 gene (Mouse), as well as a negative control, a positive control, and a FAM-labeled negative control. Components Hsf1 siRNA-1: 5 nmol (HPLC) Hsf1 siRNA-2: 5 nmol (HPLC) Hsf1 siRNA-3: 5 nmol (HPLC) siRNA Negative Control: 5 nmol (HPLC) FAM-labeled siRNA Negative Control: 5 nmol (HPLC) GAPDH siRNA Positive Control:5 nmol (HPLC)

Specifications

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Action Type

INHIBITOR

Product Line

biological_products

Application Protocols

• Item-specific tested applications, recommended dilutions, or step-by-step protocols: Not specified for this item; refer to CoA/Spec Sheet or product SDS/Tech Sheet.

• Note: While general transfection practices are outlined in the Reaction Conditions tab, validated, item-specific parameters (e.g., exact siRNA concentrations, cell line demos, or positive controls) are not provided in the Product Data and should be obtained from the official documentation for SKU H1479929.

Biological Roles

• Item-specific biological details: Not specified for this item; refer to CoA/Spec Sheet.

• General background on Hsf1 (literature/general):

  • Hsf1 (Heat shock factor 1) is a master transcriptional regulator of the heat-shock response. Upon proteotoxic stress, Hsf1 trimerizes, acquires DNA-binding competence, and activates transcription of heat-shock protein (HSP) chaperones and co-chaperones that support protein folding and proteostasis.
  • In murine systems, Hsf1 influences expression of genes such as Hsp70, Hsp90, and small HSPs, thereby modulating cellular resilience to elevated temperature, oxidative stress, and protein misfolding.
  • Hsf1 activity is modulated by post-translational modifications (phosphorylation, acetylation, sumoylation) and feedback from chaperone occupancy.
  • siRNA-mediated suppression of Hsf1 is a powerful approach to dissect stress-response circuitry, test compensatory roles of Hsf2/Hsf4, and evaluate downstream impacts on translation, protein quality control, and aggregate clearance.

• Experimental considerations (general):

  • Because Hsf1 regulates global stress programs, transient knockdown can affect basal housekeeping processes; rigorous controls and time-course experiments are recommended.
  • Combine transcriptomic (RNA-seq) or targeted qPCR with proteomic readouts to capture both immediate-early and delayed consequences of Hsf1 suppression.

Buffer Applications

• Item-specific buffer instructions: Not specified for this item; refer to CoA/Spec Sheet.

• General buffer systems for siRNA (literature/general):

  • Reconstitution/storage buffers:
    • RNase-free water (pH ~7): maximal compatibility with most transfection workflows.
    • 1× TE (10 mM Tris·HCl, 1 mM EDTA, pH 7.5–8.0): improved stability for long-term frozen stocks; avoid EDTA if downstream complexation with divalent cation–based carriers is required.
  • Working solutions: Prepare 10–50 µM stock, dilute into serum-free or serum-reduced medium during complex formation per transfection reagent instructions, then add to complete medium after 15–30 min incubation (general practice).
  • Annealing buffer (if single strands are supplied): 100 mM potassium acetate, 30 mM HEPES-KOH pH 7.4, 2 mM magnesium acetate; heat to 90 °C for 1 min and cool slowly (note: duplex siRNA is typically supplied pre-annealed; this item’s format is not specified).

• Practical tips:

  • Maintain RNase-free conditions; avoid repeated freeze–thaw by preparing aliquots.
  • Verify buffer compatibility with the chosen transfection reagent and cell line to preserve viability and maximize knockdown.

Green Alternatives

• Context: siRNA handling and use are predominantly aqueous and do not typically require hazardous organic solvents; "green" considerations focus on minimizing waste and avoiding toxic auxiliaries.

• Greener practice suggestions (general):

  • Prefer water/Tris buffers over organic cosolvents; validate transfection in serum-containing media to avoid additional diluents.
  • Use low-toxicity, biodegradable transfection reagents when possible; optimize to reduce reagent excess and cell stress.
  • Consolidate assays to reduce plastic consumable waste; choose low-retention, recyclable plastics where permitted.
  • Decontaminate with non-chlorinated RNase removal solutions when effective.

• Illustrative comparison (general):

  • Transfection with aqueous complexes vs. solvent-assisted delivery:
    • Solvent-free aqueous complexes: highest biocompatibility, minimal hazardous waste.
    • Solvent-assisted methods (e.g., DMSO): can increase cytotoxicity and complicate disposal; reserve for edge cases after optimization.

• Note: No item-specific formulation constraints are provided for SKU H1479929; consult the CoA/Spec Sheet and SDS for definitive guidance.

Pharmaceutical Uses

• Item-specific pharmacopeial/formulation status: Not specified for this item; refer to CoA/Spec Sheet.

• General, non-clinical context (literature/general):

  • siRNAs are used in research-scale formulation development to study delivery systems (e.g., lipid nanoparticles, polymers) and excipient effects (buffers, sugars, tonicity agents) under controlled laboratory conditions.
  • Typical excipients for research formulations include citrate or acetate buffers (pH 4–6 for LNP assembly), Tris buffers (pH 7–8 for storage), and cryoprotectants (sucrose, trehalose) to stabilize particles during freezing.
  • Parameters such as polydispersity, encapsulation efficiency, and RNase protection are evaluated to benchmark delivery performance in vitro.

• Note: This product is labeled “For research use only.” No medical or clinical applications are claimed or supported.

Physical Properties

• Item-specific physical properties (from Product Data):

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight (exact): Not specified for this item; refer to CoA/Spec Sheet.
  • Other specs (BP/MP/density/refractive index/logP/pKa): Not specified for this item; refer to CoA/Spec Sheet.

• General properties of siRNA (literature/general):

  • Approximate size: A 21-mer siRNA duplex typically has an aggregate MW on the order of 13–14 kDa (sequence- and modification-dependent).
  • Solubility: Freely soluble in RNase-free water and low-ionic-strength, nuclease-free buffers (e.g., 1× TE, 10 mM Tris·HCl, pH 7.5–8.0).
  • Stability: Most lyophilized siRNAs are stable at −20 °C protected from moisture; in solution, stability improves at ≤−20 °C and pH ~7–8; repeated freeze–thaw can degrade RNA.
  • UV characteristics: Typical RNA oligo quantification at A260 (1 A260 ≈ 40 µg/mL for single-stranded RNA; duplex extinction varies by sequence; exact factor is sequence-specific).
  • Hygroscopicity: Highly moisture sensitive when lyophilized; exposure to ambient humidity can reduce shelf life.

• Practical notes (general):

  • Reconstitute with RNase/DNase-free water or buffer; mix gently (do not vortex harshly) to avoid shearing.
  • Filter sterilization is generally unnecessary if handled aseptically with nuclease-free materials.

Quality and Grades

• Item-specific quality statements (from Product Data):

  • Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

• General context for siRNA quality (literature/general):

  • Purification levels: Oligonucleotides can be supplied as desalted, cartridge-purified, PAGE-purified, or HPLC-purified. Higher-purity (HPLC/PAGE) products reduce truncated species, improving knockdown performance and reproducibility.
  • Identity confirmation: Typically by MALDI-TOF/ESI-MS and analytical HPLC/UPLC; sequence verification may involve enzymatic digestion or hybridization assays.
  • Residuals and QC: Key metrics include OD260, concentration, yield, residual salts/solvents, endotoxin (for sensitive cell systems), and nuclease contamination checks. For pooled sets, individual duplex performance is often reported.
  • Stabilizers/lyoprotectants: Some suppliers include volatile buffers (e.g., ammonium acetate) or sugars; for this item, stabilizers are not specified.

• Practical implications:

  • For demanding applications (primary cells, in vivo-like systems), prefer the highest available purification grade and verify endotoxin and RNase levels.
  • Always consult the item’s CoA/Spec Sheet for exact purification method, sequence, and analytical results relevant to this SKU (H1479929).

Reaction and Applications

• Item-specific application statement (from Product Data):

  • Manufacturer Applications: Not specified for this item; refer to CoA/Spec Sheet.

• General applications for Hsf1-targeting siRNA (literature/general):

  • RNA interference (RNAi): Sequence-specific silencing of mouse Hsf1 to study heat-shock response regulation, proteostasis networks, and transcriptional stress pathways in cell culture or ex vivo models.
  • Pathway interrogation: Assess dependence of chaperone gene expression on Hsf1; evaluate compensatory activation of other HSF family members.
  • Phenotypic assays: Probe impacts on protein-folding capacity, aggregation propensity, and stress granule dynamics under thermal or proteotoxic stress.
  • Validation workflows: Combine siRNA with qRT-PCR and immunoblotting to quantify Hsf1 mRNA and protein knockdown; perform rescue with siRNA-resistant Hsf1 constructs to confirm on-target effects.
  • Screening: Using a set (Set A) enables parallel testing of multiple duplexes to identify the most potent/least off-target sequence in specific mouse cell lines.

• Practical guidance (general):

  • Optimize transfection reagent, siRNA dose (commonly 5–50 nM final), and exposure time (24–72 h) per cell type.
  • Include controls: non-targeting siRNA, positive knockdown control, and transfection reagent-only control.
  • Minimize off-targets by using pooled low-dose duplexes, chemical modifications that reduce seed-based effects, and stringent data normalization.

Reaction Conditions

For siRNA, "reaction conditions" map to transfection and gene-silencing setup in cell culture.

• Item-specific conditions: Not specified for this item; refer to CoA/Spec Sheet.

• General transfection conditions (literature/general):

  • Cell density: Plate to reach ~50–70% confluence at the time of transfection for adherent lines; optimize for each cell type.
  • siRNA dose: 5–50 nM final concentration is common; start with 10 nM and titrate based on knockdown/viability.
  • Reagent ratio: Follow supplier guidance for the chosen transfection reagent; typical N:P or µL reagent per pmol siRNA ratios require optimization.
  • Complex formation: Mix siRNA with reagent in serum-free medium; incubate 10–30 min at RT to allow complexation before adding to cells.
  • Incubation: 24–72 h post-transfection to assess mRNA/protein reduction; some targets require 96 h for maximal protein depletion.
  • Temperature/atmosphere: Standard cell culture conditions (37 °C, 5% CO2) unless using specialized systems.
  • Controls: Non-targeting siRNA, positive control siRNA, and mock-transfected cells.

• Expected outcomes (general):

  • Effective designs can yield >70% mRNA knockdown by qRT-PCR at 24–48 h and corresponding protein reduction thereafter; results are cell line– and sequence-dependent.

• Troubleshooting tips:

  • If toxicity is observed, reduce siRNA/reagent amounts or switch to lower-toxicity carriers; verify buffer composition and endotoxin levels.

Safety and Handling

• Item-specific hazard data (from Product Data):

  • GHS classification / Signal word / H-statements / Pictograms: Not specified for this item; refer to SDS.

• General safety guidance (literature/general):

  • Expected hazards: Synthetic siRNAs are non-volatile, non-flammable solids/solutions and are generally regarded as of low acute hazard; treat as laboratory chemicals.
  • PPE: Wear lab coat, gloves (nitrile), and safety glasses. Avoid inhalation of powders/aerosols and contact with skin/eyes.
  • Hygiene: Use RNase-free technique to protect product integrity and to avoid biological contamination; clean work area with RNase decontamination solution if needed.
  • First aid (overview):
    • Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing; seek medical evaluation if irritation persists.
    • Ingestion/inhalation: Rinse mouth, move to fresh air; seek medical advice according to institutional protocols.
  • Incompatibilities: Strong oxidizers and nucleases (RNase) from contaminated surfaces, gloves, or solutions will degrade the product.
  • Environmental handling: Avoid release to drains; dispose as laboratory chemical waste per institutional and local regulations.
  • Transport/storage precautions: Ship/store cold and dry; protect from moisture and light. Use nuclease-free consumables and solutions to maintain integrity.

• Always consult the product-specific SDS for authoritative hazard, exposure, and disposal information.

Solvent Selection

• Item-specific solvent guidance (from Product Data):

  • Not specified for this item; refer to CoA/Spec Sheet.

• General recommendations for siRNA (literature/general):

  • Primary solvent: RNase-free water (e.g., nuclease-free, DEPC-treated water) provides maximal compatibility with transfection and cell culture.
  • Buffer options:
    • 1× TE (10 mM Tris·HCl, 1 mM EDTA, pH 7.5–8.0): Enhances stability during storage; EDTA chelates divalent cations that can catalyze degradation.
    • 10 mM Tris·HCl, pH 7.5: EDTA-free buffer for applications sensitive to chelators.
    • PBS (nuclease-free): Acceptable for some delivery methods; verify compatibility with transfection reagent.
  • Ionic strength effects: High salt can influence complexation with cationic lipids/polymers; prepare stock solutions in low-ionic-strength buffer and adjust after complex formation if required.
  • Organic solvents: Avoid DMSO, DMF, or alcohols in the final transfection mixture unless validated; trace DMSO (<0.1%) may be tolerated by some cells but can alter uptake/toxicity.
  • pH range: Near-neutral pH (7.0–8.0) is recommended; acidic conditions promote depurination/degradation of RNA.

• Practical tips:

  • Prepare concentrated stocks (e.g., 10–50 µM) in RNase-free water/Tris and dilute into transfection medium immediately before use.
  • Use low-binding, nuclease-free tubes and tips to minimize adsorption and degradation.

Storage and Reconstitution

• Item-specific storage/shipping (from Product Data):

  • Storage: Store at −20 °C.
  • Shipped in: Ice chest + ice pads.

• Reconstitution (general guidance; not item-specific):

  • Use RNase/DNase-free water or Tris buffer (e.g., 10 mM Tris·HCl, pH 7.5–8.0) to prepare a stock solution (commonly 10–50 µM). Gently pipette to dissolve; avoid vigorous vortexing.
  • If provided lyophilized, allow vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.
  • After initial dissolution, make single-use aliquots in low-binding, nuclease-free tubes to avoid repeated freeze–thaw cycles.

• Storage of solutions (general guidance):

  • Short-term (≤1 week): 4 °C in nuclease-free buffer.
  • Long-term: −20 °C to −80 °C; TE buffer can improve stability. Protect from light and moisture.
  • Avoid >5 freeze–thaw cycles; thaw on ice and keep cold during setup.

• Additional notes:

  • Do not store in buffers containing divalent cations or nucleases. Verify compatibility of the storage buffer with the intended transfection reagent.
  • For exact concentration, extinction coefficient, and yield, consult the CoA/Spec Sheet for SKU H1479929.

Structure and Identity

Short description: Pre-designed small interfering RNA (siRNA) duplexes targeting mouse Hsf1 (Heat shock factor 1), supplied as a set (Set A) for RNAi-mediated gene silencing.

• Item-specific identifiers from Product Data:

  • SKU: H1479929
  • Product name: Hsf1 Mouse Pre-designed siRNA Set A
  • Intended target: Mouse Hsf1 (gene symbol: Hsf1) — sequence details Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES / InChIKey / CAS / CID: Not specified for this item; refer to CoA/Spec Sheet.

• General structural features (literature/general):

  • Molecular class: Double-stranded RNA oligonucleotides, typically 19–21 bp with 2-nt 3' overhangs per strand (e.g., UU), chemically synthesized.
  • Backbone: Phosphodiester or modified phosphate linkages; may include 2'-O-methyl or phosphorothioate positions in some designs (specific modifications for this item are not specified).
  • 2D description: Two complementary antiparallel RNA strands forming a short A-form duplex, with 5' phosphate/3' hydroxyl termini unless modified.
  • Targeting concept: Guide strand complementary to Hsf1 mRNA segment to recruit RISC and promote sequence-specific mRNA cleavage or translational repression.

• Notes:

  • This is a set (multiple siRNA duplexes) to increase the probability of efficient knockdown while enabling selection of the most potent/least off-target sequence in mouse cells.

Synthetic Utility

This section is not typically applicable to siRNA reagents, which are end-use biological tools rather than synthetic building blocks or reaction solvents.

• Relevant guidance (general):
  • siRNA sets serve as functional genomics tools for target validation and pathway dissection in chemical biology workflows.
  • For chemical conjugation studies (e.g., fluorophores, ligands), siRNAs may be synthesized with terminal functional groups (amine, thiol, azide); such modifications for this specific item are not specified—consult the CoA/Spec Sheet if applicable.

Target Specificity

• Item-specific targeting details (required to report sequence, isoform, or exon coverage): Not specified for this item; refer to CoA/Spec Sheet.

• Note: For siRNA products, target specificity is defined by the guide-strand sequence and any chemical modifications that tune off-target profiles. Without the product-specific sequence information, no further claims can be made here.

Frequently Asked Questions

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.