Hsph1 Mouse Pre-designed siRNA Set A

Cat. No.: H1479916

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Hsph1 Mouse Pre-designed siRNA Set A contains three designed siRNAs for Hsph1 gene (Mouse), as well as a negative control, a positive control, and a FAM-labeled negative control. Components Hsph1 siRNA-1: 5 nmol (HPLC) Hsph1 siRNA-2: 5 nmol (HPLC) Hsph1 siRNA-3: 5 nmol (HPLC) siRNA Negative Control: 5 nmol (HPLC) FAM-labeled siRNA Negative Control: 5 nmol (HPLC) GAPDH siRNA Positive Control:5 nmol (HPLC)

Specifications

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Product Line

biological_products

Application Protocols

Item‑specific tested applications, recommended dilutions/concentrations, and validated cell lines are not provided in the Product Data for this SKU.

• For protocols and conditions verified for this product, please refer to the CoA/Spec Sheet and SDS, or contact technical support with the SKU (H1479916) for guidance.

In the absence of product‑specific instructions, follow your laboratory’s validated RNAi transfection SOPs and optimize parameters empirically for the cell model and transfection reagent in use.

Biological Roles

This siRNA set targets mouse Hsph1 (HSPH1/Hsp110), a member of the Hsp70 superfamily with nucleotide exchange factor (NEF) activity and chaperone/co‑chaperone functions.

• HSPH1 function (literature)

  • Acts as a high‑molecular‑weight heat shock protein that cooperates with Hsp70/Hsp40 systems to prevent aggregation of nascent or stress‑denatured proteins and to promote refolding.
  • Functions as a nucleotide exchange factor for Hsp70, accelerating ADP release and facilitating ATP‑dependent chaperone cycling.
  • Implicated in proteostasis, heat‑shock response, and recovery from proteotoxic stress; localizes to cytosol and can associate with stress granules.

• Pathway context (literature)

  • Interacts within the chaperone network including HSPA1A/B, HSPA8, DNAJs, and co‑chaperones BAG family members.
  • Regulated by heat shock factor 1 (HSF1) at the transcriptional level during stress responses.

• Research applications enabled by knockdown

  • Dissect contribution of HSPH1 to protein quality control, aggregate clearance, and cellular stress resilience.
  • Probe genetic interactions with the ubiquitin‑proteasome system and autophagy pathways.
  • Provide mechanistic controls in studies of chaperone modulators.

Note: The above roles summarize literature for HSPH1; they describe biological context rather than properties or performance of this specific SKU.

Buffer Applications

This product is not a buffer reagent; however, buffer choice is important for siRNA stability and performance.

• Reconstitution and storage buffers (general, literature)

  • RNase‑free water for immediate use and short‑term storage.
  • TE buffer (e.g., 10 mM Tris‑HCl, pH 7.5–8.0, 0.1–1 mM EDTA) for improved stability during long‑term frozen storage by chelating divalent cations.

• Working solutions

  • Prepare working dilutions in nuclease‑free water or buffer compatible with the transfection reagent; many lipid systems recommend low‑ionic‑strength buffers during complexation.

• Practical tips

  • Filter or autoclave buffers where appropriate; use certified RNase‑free consumables.
  • Avoid repeated freeze‑thaw by aliquoting stocks upon initial reconstitution.

Item‑specific buffer composition or any supplied buffer for this SKU is not specified; refer to the CoA/Spec Sheet for definitive instructions.

Green Alternatives

While siRNA use itself has a modest environmental footprint, transfection workflows can involve solvents, plastics, and reagents with varying EHS profiles. Consider the following greener practices (general, literature):

• Greener choices

  • Prefer aqueous, RNase‑free buffers over organic co‑solvents; avoid phenol/chloroform for RNA handling when possible.
  • Use biodegradable or lower‑toxicity transfection reagents when compatible (e.g., certain lipid or polymer formulations) to reduce cytotoxicity and hazardous waste.
  • Scale reactions appropriately to minimize consumables and waste; adopt reusable cold blocks instead of dry ice when feasible for short transfers.

• Process improvements

  • Implement nuclease‑free but non‑halogenated surface decontaminants.
  • Consolidate shipments and select ambient-stable formulations when supported by data (not specified for this item; this SKU ships cold).

• Comparison snapshot (general)

  • Chloroform‑based RNA workflows: Higher hazard, halogenated waste.
  • Column‑based or magnetic‑bead cleanup: Aqueous systems, reduced solvent waste.

Trade‑offs should be evaluated against experimental performance; maintain RNase control and siRNA integrity as primary constraints.

Pharmaceutical Uses

This product is for research use only; no use in humans or diagnostics is intended or implied.

• Formulation/manufacturing context (general)

  • siRNA duplexes are used in preclinical research to explore target validation, mechanism‑of‑action, and delivery technologies. Insights can inform later formulation development, but this SKU is not provided with GMP documentation.
  • Typical excipients in siRNA formulations (literature) include aqueous buffers, salts, sugars (cryoprotectants), and delivery vehicles such as lipids or polymers. These notes are general and not specific to this item.

• Regulatory status

  • No pharmacopeial monograph or GMP status is specified for this product.

Item-specific pharmaceutical grade, residual solvent profile, or excipient content: Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific physicochemical specifications (BP, MP, density, UV cutoff, exact MW, % water, etc.) are not provided for this siRNA set.

• Item-specific values

  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • Extinction coefficient: Not specified for this item; refer to CoA/Spec Sheet.
  • Purity/grade: Not specified for this item; refer to CoA/Spec Sheet.
  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.

• General properties of siRNA (literature/typical)

  • Physical state: Usually supplied lyophilized or in aqueous RNase‑free buffer.
  • Solubility: Freely soluble in RNase‑free water or low‑salt buffers (e.g., 10 mM Tris, pH 7–8) to ≥100 µM.
  • Stability: Stable when lyophilized at −20 °C, protected from moisture; aqueous stocks are stable at −20 °C (aliquoted) and 4 °C for short term, particularly in low‑salt TE to reduce hydrolysis.
  • pH sensitivity: Acidic conditions promote depurination/strand scission; alkaline conditions promote 2′‑OH mediated cleavage—maintain near‑neutral pH.
  • UV absorbance: Strong at 260 nm; concentration often determined by A260 using strand‑specific ε (sequence dependent; not specified for this item).

Note: For any definitive values applicable to this SKU, consult the product’s CoA/Spec Sheet.

Quality and Grades

• Item-specific quality information

  • Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.
  • Stabilizers/modifications: Not specified for this item; refer to CoA/Spec Sheet.

• Interpreting typical siRNA quality descriptors (general guidance)

  • Desalted vs. HPLC/ PAGE‑purified: HPLC or PAGE purification reduces truncated sequences and improves potency/reproducibility, especially critical for low‑dose or in vivo studies. Desalted material can be suitable for routine cell culture knockdowns at higher concentrations.
  • Duplex validation: Annealing verification (e.g., native PAGE, LC‑MS) and strand stoichiometry control support batch consistency. Check CoA for annealing status.
  • Endotoxin/RNase control: For cell‑based work, low endotoxin specifications and RNase‑free handling are important; if relevant to your workflow, request supporting test data.
  • Quantitation: Supplier may report OD260, nmol, and (optionally) extinction coefficient. Sequence determines ε and expected mass; verify on CoA for this set.

• What is specific to this item

  • “Pre‑designed siRNA Set A” indicates multiple duplexes targeting mouse Hsph1, typically selected to minimize off‑target potential and to span distinct mRNA regions. Exact design rules, sequences, and modification status are not disclosed here—consult CoA/Spec Sheet for definitive details.

Reaction and Applications

Chemical reaction use is not typical for siRNA reagents. Instead, applications center on transient, sequence‑specific knockdown of target transcripts in cells and tissues.

• Application focus (aligned to manufacturer’s category and product name)

  • Gene silencing of mouse Hsph1 (HSPH1/Hsp110) by RNA interference in mammalian cell culture models.
  • Use cases: Mechanistic studies of proteostasis and heat‑shock pathways, validation of HSPH1 function, pathway mapping, and genetic interaction screening.

• Practical considerations (general, literature)

  • Use multiple independent siRNA duplexes (as in a Set A) to control for off‑target effects; converge on phenotypes consistent across duplexes.
  • Optimize transfection: Screen lipid/electroporation conditions, cell density, and siRNA dose (often 5–50 nM final) to balance efficacy and viability.
  • Timing: Assess mRNA knockdown at 24–48 h and protein depletion at 48–96 h, depending on protein stability and turnover.
  • Controls: Include a non‑targeting control siRNA and, when feasible, a rescue experiment with an siRNA‑resistant Hsph1 construct.

• Assays

  • Quantify silencing by RT‑qPCR (Hsph1 mRNA), immunoblotting (HSPH1 protein), and functional readouts such as heat‑shock resilience or proteotoxic stress markers.

Note: Exact sequences, modification patterns, and tested cell systems for this item are not provided here; consult the CoA/Spec Sheet and SDS for item‑specific guidance.

Reaction Conditions

Classical chemical reaction conditions are not applicable. Below are general, literature‑based conditions for cell transfection with siRNA; these are not item‑specific recommendations and should be optimized per cell line and reagent.

• Typical transfection parameters (literature)

  • siRNA dose: 5–50 nM final in culture medium; some protocols succeed at 1–5 nM with highly potent designs.
  • Cell confluency: 40–70% at time of transfection for adherent lines to balance uptake and viability.
  • Complexation: Incubate siRNA with lipid/polymer transfection reagent in serum‑free medium (e.g., Opti‑MEM) for 5–20 min before adding to cells; follow reagent‑specific ratios.
  • Incubation: 4–6 h in reduced‑serum medium, then replace with complete medium; or add directly to complete medium if reagent supports it.
  • Readouts: mRNA by RT‑qPCR at 24–48 h; protein by Western blot at 48–96 h.

• Controls and replication

  • Include non‑targeting and positive‑control siRNAs, and assess cytotoxicity (e.g., CellTiter‑Glo) alongside knockdown.
  • Use at least three biological replicates and two independent duplexes targeting Hsph1 to mitigate off‑target artifacts.

Consult the CoA/Spec Sheet for any item‑specific recommendations when available.

Safety and Handling

• Hazard classification (item-specific)

  • GHS classification, signal word, pictograms, and H‑statements: Not specified for this item; refer to SDS.

• General safety profile (oligonucleotide reagents)

  • siRNA duplexes are generally considered low hazard for laboratory handling. Avoid inhalation or contact with skin/eyes; treat as a laboratory chemical of unknown toxicity.
  • Use standard PPE: lab coat, nitrile gloves, safety glasses. Work in a clean area designated RNase‑free to prevent degradation.

• Handling guidance

  • Avoid repeated freeze‑thaw. Prepare single‑use aliquots in RNase‑free tubes.
  • Use dedicated RNase‑free tips, tubes, and solutions. Clean benches and tools with RNase decontamination solutions to minimize nuclease contamination.
  • Do not vortex excessively after reconstitution; gentle pipetting helps maintain duplex integrity.

• Incompatibilities (general)

  • Avoid strong acids/bases, oxidants, and nucleases (RNase A and environmental RNases). Avoid metal contamination that can catalyze RNA hydrolysis.

• First‑aid overview (general)

  • Skin/eye contact: Rinse with water for at least 15 minutes; remove contaminated clothing.
  • Ingestion/inhalation: Move to fresh air, rinse mouth; seek medical attention if symptoms occur.

Always consult the product SDS for authoritative safety and regulatory information.

Solvent Selection

This product is an RNA oligonucleotide reagent; organic solvent selection is not generally applicable. Reconstitution and dilution are typically performed in nuclease‑free aqueous media.

• Practical guidance (general, literature)

  • Preferred: RNase‑free water or 1×/10 mM Tris (pH 7.5–8.0) with 0.1–1 mM EDTA (TE) to chelate divalent cations and enhance stability.
  • Avoid: Acidic buffers (RNA depurination), strong bases (2′‑OH cleavage), and solutions with contaminating RNases or heavy metals.
  • Salt considerations: Keep ionic strength low to moderate during stock preparation; follow transfection reagent recommendations for final complexation buffers.

• Comparison (general)

  • RNase‑free water: Maximal simplicity and compatibility; slightly less stabilizing than TE during long storage.
  • TE (10 mM Tris, 0.1–1 mM EDTA): Improved long‑term stability; verify EDTA compatibility with downstream transfection chemistry.

For exact buffer recommendations specific to this SKU, refer to the product’s Spec Sheet.

Storage and Reconstitution

• Item-specific storage

  • Storage condition: Store at −20 °C (per Product Data).
  • Shipping: Ice chest + ice pads (per Product Data).

• Reconstitution (general, literature)

  • Use RNase‑free water or TE buffer (e.g., 10 mM Tris‑HCl, pH 7.5–8.0, 0.1–1 mM EDTA). Gently spin down lyophilized material before opening. Add calculated volume to achieve a convenient stock (e.g., 10–100 µM), mix gently, and allow to sit on ice for 10–15 min to fully dissolve.
  • If supplied in solution, keep on ice during handling; avoid vigorous vortexing.

• Aliquoting and storage after reconstitution (general)

  • Prepare single‑use aliquots in low‑bind, RNase‑free tubes. Store at −20 °C (or −80 °C for long‑term) protected from light and moisture.
  • Avoid repeated freeze–thaw; thaw on ice and keep cold during setup.
  • For short‑term (≤1 week), 4 °C storage in TE can be acceptable; verify performance.

• Concentration determination (general)

  • Quantify by A260 using appropriate pathlength and an extinction coefficient for each strand (sequence dependent; not specified for this item). Alternatively, use supplier‑reported nmol/OD.

For any item‑specific buffer composition, concentration, or stabilizers, consult the product’s CoA/Spec Sheet.

Structure and Identity

Pre‑designed siRNA duplex set targeting the mouse Hsph1 gene (also known as HSP110/HSPH1), supplied as a research oligonucleotide reagent for transient gene knockdown.

• Item-specific identifiers

  • SKU: H1479916
  • Product name: Hsph1 Mouse Pre-designed siRNA Set A
  • Target: Mouse Hsph1 (HSPH1/Hsp110) mRNA
  • Format: Pre-designed siRNA set (multiple sequence duplexes; exact sequences not provided here)
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES / InChI / CAS / CID: Not applicable to heterogeneous, sequence-defined oligoribonucleotide mixtures; not specified for this item.

• Structural features (general, literature)

  • Double‑stranded ~21–23 nt RNA duplexes with 2-nt 3′ overhangs are typical for siRNA designs (literature), composed of ribonucleotides linked by 3′–5′ phosphodiester or modified phosphorothioate linkages depending on design.
  • May include optional chemical stabilizations (e.g., 2′‑O‑Me, 2′‑F, PS) in some platforms; not specified for this item.

• 2D description in words (general)

  • Each siRNA comprises an antisense (guide) strand complementary to the target Hsph1 mRNA region and a sense (passenger) strand; when delivered into cells, the guide strand is loaded into RISC to direct sequence‑specific cleavage of Hsph1 transcripts (mechanism per RNAi literature).

Synthetic Utility

Not a synthetic building block or chemical intermediate. As a sequence‑defined biological reagent, this siRNA set is intended for functional genomics and gene‑silencing studies rather than for use in traditional organic synthesis.

• Related laboratory utilities (general)
  • Can serve as a tool to validate chemical probe selectivity by comparing phenotypes from pharmacological inhibition vs. genetic knockdown of Hsph1.
  • Useful in dissecting pathway dependencies to guide target engagement assays and structure–activity relationship (SAR) interpretation for small‑molecule campaigns.

For small‑molecule synthesis or reaction development, refer to solvent and reagent categories more suitable to chemical transformations.

Target Specificity

• Target gene: Hsph1 (also known as HSPH1/Hsp110)
• Species: Mus musculus (mouse)
• Modality: Pre‑designed siRNA duplex set (Set A) targeting Hsph1 mRNA
• Intended effect: Sequence‑directed RNAi‑mediated knockdown of Hsph1 transcripts in mouse cells
• Sequences/target exons: Not specified for this item; refer to CoA/Spec Sheet.
• Off‑target minimization: Pre‑designed sets typically include multiple siRNAs to confirm on‑target phenotypes; exact design criteria are not provided here.

Note: No antibody clone/isotype or protein epitope information applies, as this is an siRNA reagent.

Frequently Asked Questions

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.