Human Complement Factor D ELISA Kit

Cat. No.: H1510017

GRADE & PURITY BioReagent

Overview

This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Human Complement Factor D concentration in samples. The Human Complement Factor D capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Human Complement Factor D present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Human Complement Factor D is added. This antibody binds to the captured Human Complement Factor D, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Human Complement Factor D is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofHuman Complement Factor D is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofHuman Complement Factor D, and the concentration of Human Complement Factor D in unknown samples is interpolated from this curve based on their OD values.

Specifications

Specifications & Purity

BioReagent

Grade

BioReagent

Application

ELISA

Sample type

body fluids, cell culture supernatants, Plasma, Serum, tissue homogenates

Detection Range

3.12 pg/mL~200 pg/mL

Method Of Detection

Sandwich ELISA

Detected Species Object

Human

Specificity

Detecting Human Complement Factor D in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Storage and Shipping

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ for 6 months.

Frequently Asked Questions

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.