Hyaluronidase, CAS No.37326-33-3

CAS: 37326-33-3 Cat. No.: H754463 EC Number: 253-464-3

GRADE & PURITY Bioactive, ActiBioPure™, Native, Endotoxin tested, High Performance, EnzymoPure™, ≥1000 IU/mg，derived from bovine testicles

Accession #

Q5E985,Q8SQG8

Endotoxin Concentration

<0.2 EU/IU

Bioactivity

≥1000 IU/mg enzyme powder

Overview

Hyaluronidase is widely present in nature.In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards.

Application

Hyaluronidase from Streptomyces hyalurolyticus has been used:in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue；as a component of digestion solution for the derivation of germline stem cells from testicular tissue.

Specifications

Product Name

Hyaluronidase, CAS No.37326-33-3

Synonyms

Hyaluronate Lyase | hyaluronoglucosaminidase | chondroitinase | chondroitinase I | hyaluronidase | hyaluronoglucosidase | Hyaluronate 4-glycanohydrolase | HYAL1 | HYAL2

Grade

ActiBioPure™, Bioactive, Endotoxin tested, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive,ActiBioPure™,Native,High Performance,EnzymoPure™,endotoxin tested,≥1000 IU/mg，derived from bovine testicles

Biochemical and Physiological Mechanisms

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression. These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Bioactivity

≥1000 IU/mg enzyme powder

Endotoxin Concentration

<0.2 EU/IU

Accession #

Q5E985,Q8SQG8

CAS

37326-33-3

Enzyme Commission Number

3.2.1.35

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥1000 IU/mg，derived from bovine testicles

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

Under conditions of 37 ℃ and pH 5.3-5.7, one unit can cause a 0.330% change in transmittance of 2.0 mL reaction mixture at 600 nm (detected at 45 min)