GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.5 μL/test
Flow Cytometry: Human peripheral blood lymphocytes
Conjugation
FITC
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
Grade
ExactAb™, Validated
Purification Method
Protein G purified
Concentration
5 μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
IFN-γ Mouse mAb (FITC) (Ab191775) - Flow Cytometry Expression of IFN-γ by stimulated human peripheral blood lymphocyte cells (PBMC). Human PBMC were stimulated for 5 hours with PMA (50 ng/ml) plus ionomycin (1 μg/mL) and brefeldin A (3 μg/mL) for 2 hours. The PBMC were stained with CD3 Mouse mAb (AF647) (Ab189663), fixed with 2% PFA for 30 minutes, permeabilized with 0.5% triton X-100 for 30 minutes, then subsequently stained with 0.1 µg of IFN-γ Mouse mAb (FITC) (Ab191775; Right panel). Unstimulated Human PBMC were used as control (Left panel).
IFN-γ Mouse mAb (FITC) (Ab191775) - Flow Cytometry Expression of IFN-γ by stimulated human peripheral blood lymphocyte cells (PBMC). Human PBMC were stimulated for 5 hours with PMA (50 ng/ml) plus ionomycin (1 μg/mL) and brefeldin A (3 μg/mL) for 2 hours. The PBMC were stained with CD3 Mouse mAb (AF647) (Ab189663), fixed with 2% PFA for 30 minutes, permeabilized with 0.5% triton X-100 for 30 minutes, then subsequently stained with 0.1 µg of IFN-γ Mouse mAb (FITC) (Ab191775; Right panel). Left panel - Mouse IgG (FITC) (Ab180307).
Application
Application
Dilution info
Flow Cytometry
5μl/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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