IMDM - sterile-filtered, for cell culture, With stable glutamine and sodium bicarbonate, liquid

Cat. No.: I755863

GRADE & PURITY Sterile-filtered, for Cell culture, With stable glutamine and sodium bicarbonate, liquid

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application:

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Specifications

Specifications & Purity

sterile-filtered, for cell culture, With stable glutamine and sodium bicarbonate, liquid

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

for Cell culture, Sterile-filtered