RNF43 Mouse mAb: Application Guide for Research Use

RNF43 Mouse mAb is a monoclonal antibody raised in mouse and designed to specifically detect the RNF43 protein, an E3 ubiquitin ligase critical to the negative regulation of Wnt signaling pathways in normal and cancerous tissues.

Product SKU

Ab351057

Antibody Type

Mouse Monoclonal Antibody (mAb)

Formulation

PBS only (no carrier protein, no preservative)

Target Protein

RNF43 (Ring Finger Protein 43)

Host Species

Mouse

Reference Catalog

absea#KC-3394

Storage Buffer

PBS

Target Overview: RNF43 and Its Biological Significance

RNF43 (Ring Finger Protein 43) is a transmembrane E3 ubiquitin ligase that plays a pivotal role in the negative regulation of the Wnt signaling pathway. By promoting the ubiquitination and subsequent degradation of Frizzled receptors, RNF43 acts as a key brake on canonical Wnt activity. Loss-of-function mutations in RNF43 are frequently observed in pancreatic ductal adenocarcinoma, colorectal cancer, endometrial cancer, and other malignancies, making it a clinically relevant tumor suppressor target.

Detection of RNF43 protein expression is important for studies of Wnt pathway dysregulation, cancer biology, and potential therapeutic intervention. The RNF43 Mouse mAb (Ab351057) provides a reliable tool for such investigations.

Product Description and Formulation

The RNF43 Mouse mAb (Ab351057, absea reference KC-3394) is a mouse-derived monoclonal antibody formulated in phosphate-buffered saline (PBS) only, without added carrier proteins such as BSA or preservatives such as sodium azide. This carrier-free, preservative-free formulation is particularly suitable for applications where additional proteins could cause interference, such as conjugation reactions, single-cell studies, or highly sensitive immunoassays.

Key formulation details:

• Buffer: PBS
• No carrier protein added
• No preservative added
• Host species: Mouse
• Antibody format: Monoclonal IgG (class to be confirmed with manufacturer datasheet)

Note: Users should consult the official Aladdin Scientific product datasheet for confirmed isotype, clone identity, concentration, and lot-specific quality control data.

Recommended Applications and Usage Guidelines

Mouse monoclonal antibodies targeting RNF43 are commonly employed in a range of standard immunological techniques. Researchers should validate the antibody for each intended application in their specific experimental system. Typical applications for anti-RNF43 monoclonal antibodies include:

• Western Blotting (WB): Detection of RNF43 protein in whole-cell or tissue lysates. Recommended starting dilution should be determined empirically; consult the product datasheet for suggested ranges.
• Immunohistochemistry (IHC): Localization of RNF43 in formalin-fixed, paraffin-embedded (FFPE) or frozen tissue sections relevant to cancer pathology studies.
• Immunofluorescence (IF): Subcellular localization of RNF43 in cultured cells, relevant to studying its membrane-associated function.
• Immunoprecipitation (IP): Pull-down of RNF43 protein complexes for interaction studies.

Important: Confirm validated applications on the official product datasheet (Ab351057). Optimal antibody concentrations and conditions vary by application and must be determined by the end user.

Storage, Handling, and Stability

Proper storage and handling are essential to maintain antibody activity and ensure reproducible experimental results. The following general guidelines apply to the RNF43 Mouse mAb (Ab351057) formulated in PBS:

• Short-term storage (up to 1 month): Store at 4 °C. Avoid repeated freeze-thaw cycles.
• Long-term storage: Aliquot into single-use volumes and store at −20 °C or −80 °C. Freezing in a non-frost-free freezer is preferred to avoid temperature fluctuations.
• Carrier-free formulation note: Because this antibody contains no BSA or other stabilizing carrier proteins, it may be more susceptible to degradation during storage or at low concentrations. Consider adding a compatible carrier protein if long-term stability is a concern and the application allows.
• Avoid: Prolonged exposure to light, repeated freeze-thaw cycles, and storage at room temperature.
• Sterility: Handle under aseptic conditions if using in cell-based assays.

Always refer to the official Aladdin Scientific product datasheet for lot-specific storage recommendations and expiry information.

Quality Control and Troubleshooting

Consistent antibody performance depends on rigorous quality control at both the manufacturer and user levels. When working with the RNF43 Mouse mAb (Ab351057), consider the following quality assurance and troubleshooting practices:

Positive controls: Use cell lines or tissues known to express RNF43 (e.g., colorectal or pancreatic cell lines with wild-type RNF43) as positive controls in each experiment.

Negative controls: Include an isotype-matched mouse IgG control at the same concentration to assess non-specific background.

Common troubleshooting scenarios:

• No signal / weak signal: Check antibody concentration, incubation time and temperature, and confirm that the target is expressed in your model system. Verify that the secondary antibody is compatible with mouse primary antibodies.
• High background: Optimize blocking conditions (increase blocking agent concentration or duration). Increase wash stringency. Titrate the primary antibody.
• Multiple non-specific bands (WB): Optimize lysis conditions; confirm sample integrity. Consider increasing antibody dilution.

Contact Aladdin Scientific technical support for lot-specific troubleshooting assistance.

Frequently asked questions

What is the formulation of RNF43 Mouse mAb (Ab351057)?

The RNF43 Mouse mAb (Ab351057) is formulated in PBS only, with no added carrier proteins (such as BSA) and no preservatives. This carrier-free format makes it suitable for conjugation applications and sensitive assays where extraneous proteins could interfere.

Which applications is the RNF43 Mouse mAb validated for?

Validated applications should be confirmed on the official Aladdin Scientific product datasheet for Ab351057. Common applications for anti-RNF43 monoclonal antibodies include Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), and Immunoprecipitation (IP). Users should empirically optimize conditions for each application.

How should I store the RNF43 Mouse mAb to maintain its activity?

For short-term use (up to one month), store at 4 °C. For long-term storage, aliquot into single-use volumes and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles. Since this antibody is carrier-free, consider adding a compatible stabilizer if long-term stability is required and the downstream application permits.

What positive control should I use when working with RNF43 Mouse mAb?

Use cell lines or tissue samples known to express RNF43, such as wild-type colorectal or pancreatic cancer cell lines. Always pair with an isotype-matched mouse IgG negative control at the same concentration to distinguish specific from non-specific signals.

Is the RNF43 Mouse mAb suitable for use with secondary antibodies raised against mouse IgG?

Yes. Since the RNF43 Mouse mAb is a mouse monoclonal antibody, it is compatible with any high-quality anti-mouse IgG secondary antibody. Ensure the secondary antibody is validated for your specific application (e.g., HRP-conjugated for WB, fluorophore-conjugated for IF/IHC).

References

1. Hao HX, et al. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Nature. 2012;485(7397):195-200. doi:10.1038/nature11019
2. Koo BK, et al. Tumour suppressor RNF43 is a stem-cell E3 ligase that induces endocytosis of Wnt receptors. Nature. 2012;488(7413):665-669. doi:10.1038/nature11308
3. Aladdin Scientific. RNF43 Mouse mAb (Ab351057) Product Datasheet. Aladdin Scientific; [date not specified].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.