Recombinant Human GKN1 Protein: Key Facts & FAQs

Recombinant Human GKN1 Protein is a recombinantly produced form of gastrokine 1, a gastric mucosal-secreted protein implicated in the maintenance of stomach epithelial homeostasis and studied for its potential tumor-suppressive roles in gastric cancer research.

Product Name

Recombinant Human GKN1 Protein

SKU

rp367953

Gene Name

GKN1 (also known as Gastrokine 1, AMP18)

Species Origin

Human (Homo sapiens)

Protein Function

Gastric mucosal protection, epithelial homeostasis, putative tumor suppressor

Primary Research Application

Gastric cancer biology, mucosal integrity studies, in vitro cell-based assays

What Is GKN1 and Why Is It Scientifically Important?

GKN1, or Gastrokine 1, is a protein encoded by the GKN1 gene and is predominantly expressed in the mucosal epithelium of the stomach. It was initially identified as an 18 kDa antrum mucosal protein (AMP18) and is thought to play a critical role in maintaining the integrity and homeostasis of the gastric epithelial lining.

Numerous studies have reported that GKN1 expression is markedly downregulated or absent in gastric cancer tissues compared to normal gastric mucosa, suggesting a tumor-suppressive function. This pattern has made GKN1 a subject of active investigation in the fields of gastric oncology, mucosal biology, and potential therapeutic target research.

Recombinant Human GKN1 Protein enables researchers to perform controlled in vitro experiments, including cell proliferation assays, apoptosis studies, and protein–protein interaction analyses, without relying on endogenous expression systems.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human GKN1 Protein (SKU: rp367953) is produced using recombinant expression technology to ensure batch-to-batch consistency suitable for rigorous scientific research. Key specification details, including the precise molecular weight, expression host system, tag information, formulation buffer, and measured purity, should be confirmed with the official product datasheet or by contacting Aladdin Scientific's technical support team, as these parameters may be updated.

Researchers should verify the following parameters from the current datasheet before experimental use:

• Expression Host: Confirm whether produced in E. coli, baculovirus/insect cells, mammalian cells, or yeast (refer to datasheet).
• Purity: Typically assessed by SDS-PAGE and/or HPLC; expected to be >90% for research-grade recombinant proteins (verify with CoA).
• Tag: Presence and position of any affinity tag (e.g., His-tag) should be confirmed per datasheet.
• Endotoxin Level: Critical for cell-based assays; confirm acceptable levels in the Certificate of Analysis (CoA).

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins are essential to preserve biological activity and ensure reliable experimental results. While specific conditions must be confirmed with the product datasheet for Recombinant Human GKN1 Protein (rp367953), the following general best practices apply to most research-grade recombinant proteins:

• Short-term storage (up to 4 weeks): Store at 4°C in the supplied formulation buffer.
• Long-term storage: Aliquot into single-use volumes and store at -20°C or -80°C to avoid repeated freeze-thaw cycles, which can degrade protein activity.
• Carrier protein addition: If the protein is supplied at low concentrations, addition of a carrier protein such as 0.1% BSA may help prevent adsorption losses.
• Avoid repeated freeze-thaw: Each freeze-thaw cycle can reduce biological activity. Prepare working aliquots upon first thaw.

Always consult the specific product datasheet and Certificate of Analysis (CoA) supplied with each lot for authoritative storage instructions.

Recommended Research Applications

Recombinant Human GKN1 Protein is suitable for a range of in vitro and biochemical research applications. Common uses in the scientific literature and standard laboratory practice include:

• Cell Proliferation and Apoptosis Assays: GKN1 has been studied for its ability to inhibit proliferation and induce apoptosis in gastric cancer cell lines. Recombinant GKN1 can be added exogenously to cell culture systems to study these effects.
• Western Blotting (as Positive Control): Can be used as a positive control or standard in Western blot experiments targeting GKN1.
• ELISA Standard: May serve as a calibration standard in sandwich ELISA assays designed to quantify GKN1 levels in biological samples.
• Protein–Protein Interaction Studies: Useful for pull-down assays or co-immunoprecipitation to map GKN1 interaction partners.
• Functional Rescue Experiments: Suitable for reconstitution experiments in GKN1-deficient cellular models.

Researchers are advised to optimize reagent concentrations and conditions for each specific application. Application compatibility should be confirmed in the product datasheet.

Ordering and Technical Support

Recombinant Human GKN1 Protein is available from Aladdin Scientific under SKU rp367953. To place an order or request a quotation, customers can visit the Aladdin Scientific website, contact their regional sales representative, or reach out to the customer service team directly.

For technical inquiries—including questions about experimental protocols, product compatibility, or custom formulation requests—Aladdin Scientific's technical support team is available to assist. When contacting support, please have the SKU number (rp367953) and lot number from your product vial ready to facilitate efficient assistance.

Bulk quantities, custom concentrations, or special formulations may be available upon request. Certificate of Analysis (CoA) and Safety Data Sheet (SDS) documents can typically be downloaded from the product page or requested from customer service.

Frequently asked questions

What is GKN1 protein and what is its biological function?

GKN1 (Gastrokine 1) is a secreted protein primarily expressed in the gastric antral mucosa, where it plays a role in maintaining epithelial integrity and homeostasis. It has been widely studied as a putative tumor suppressor in gastric cancer, as its expression is frequently lost in gastric carcinoma tissues. Recombinant Human GKN1 Protein enables controlled in vitro investigation of these biological functions.

What expression system is used to produce Recombinant Human GKN1 Protein (rp367953)?

The specific expression host system for Recombinant Human GKN1 Protein (rp367953) should be confirmed in the official product datasheet available from Aladdin Scientific, as expression systems can vary (e.g., E. coli, insect cells, mammalian cells). Please download the current datasheet from the product page or contact technical support for the most accurate and up-to-date information.

How should Recombinant Human GKN1 Protein be stored to maintain activity?

In general, recombinant proteins should be aliquoted into single-use volumes upon receipt and stored at -80°C for long-term preservation, avoiding repeated freeze-thaw cycles which can compromise biological activity. Short-term storage at 4°C is typically acceptable for up to a few weeks. Always refer to the specific storage instructions provided on the Aladdin Scientific product datasheet and Certificate of Analysis for authoritative guidance.

What research applications is Recombinant Human GKN1 Protein validated for?

Recombinant Human GKN1 Protein is commonly used in cell-based functional assays (e.g., proliferation and apoptosis studies in gastric cancer cell lines), Western blot as a positive control, ELISA as a calibration standard, and protein–protein interaction studies such as pull-down assays. Validated applications for the specific lot should be confirmed in the product datasheet available from Aladdin Scientific.

How can I obtain a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) for rp367953?

Certificates of Analysis and Safety Data Sheets for Recombinant Human GKN1 Protein (rp367953) can typically be downloaded directly from the product page on the Aladdin Scientific website using your lot number, or requested by contacting Aladdin Scientific's customer service team. Having the SKU (rp367953) and lot number ready will expedite the process.

References

1. Nardone G, et al. Gastrokine 1 expression is reduced in gastric carcinoma and its reintroduction inhibits the tumour cell proliferation. European Journal of Cancer. 2007.
2. Martin TE, et al. AMP-18, a novel gastrokine, is a secreted protein of the gastric antrum. American Journal of Physiology - Gastrointestinal and Liver Physiology. 2003.
3. Aladdin Scientific. Recombinant Human GKN1 Protein (rp367953) Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.