Recombinant Human GINS3 Protein: Key Facts & FAQs

Recombinant Human GINS3 Protein is a key research reagent representing the PSF3 subunit of the tetrameric GINS complex, which is essential for the initiation and elongation phases of eukaryotic DNA replication.

Product Name

Recombinant Human GINS3 Protein

SKU

rp368337

Also Known As

PSF3; GINS complex subunit 3

Species

Human (Homo sapiens)

Protein Function

Subunit of the GINS complex required for DNA replication initiation and replisome progression

Content Type

Recombinant Protein

Supplier

Aladdin Scientific

What Is GINS3 and Why Does It Matter in Research?

GINS3, also designated PSF3 (Partner of SLD5, Four subunit 3), is one of four subunits that form the heterotetrameric GINS complex (composed of SLD5, PSF1, PSF2, and PSF3). The GINS complex is a highly conserved component of the eukaryotic replisome and plays an indispensable role in both the initiation and elongation steps of chromosomal DNA replication.

GINS3 physically interacts with CDC45 and the MCM2–7 helicase ring to form the CMG (CDC45–MCM–GINS) holohelicase, the molecular motor responsible for unwinding double-stranded DNA ahead of the replication fork. Research into GINS3 function is therefore central to understanding genome stability, cell cycle regulation, and the molecular basis of replication stress.

Elevated GINS3 expression has been reported in several cancer types, making Recombinant Human GINS3 Protein a valuable tool for oncology-focused biochemical and structural studies.

Product Specifications and Quality Attributes

Recombinant Human GINS3 Protein (SKU: rp368337) is produced recombinantly to support a broad range of in vitro research applications. Researchers should consult the official product datasheet for the most current and verified specifications, including the following parameters:

• Expression System: Confirmed on the product datasheet; common systems for GINS subunits include E. coli or baculovirus/insect cell platforms.
• Purity: Typically ≥85–95% as determined by SDS-PAGE; exact figure stated on the Certificate of Analysis (CoA).
• Formulation: Supplied in a buffered solution; specific buffer composition and any carrier proteins or stabilizers are detailed on the datasheet.
• Molecular Weight: Refer to the CoA and datasheet; the theoretical molecular weight of the human GINS3 polypeptide is approximately 25 kDa, though recombinant constructs with tags may migrate differently.
• Endotoxin Level: Specified on the CoA; low-endotoxin preparations are recommended for cell-based assays.

Please download the latest datasheet from the Aladdin Scientific website or contact technical support for a batch-specific CoA.

Recommended Applications and Usage Guidelines

Recombinant Human GINS3 Protein is suitable for a variety of research applications. Optimal conditions should be determined empirically for each experimental system:

• Protein–Protein Interaction Studies: Used to interrogate binding partners within the CMG holohelicase or to identify novel GINS3-interacting proteins via pull-down or co-immunoprecipitation assays.
• Biochemical Activity Assays: Functional reconstitution of the GINS complex or CMG helicase in vitro.
• Structural Biology: Suitable as a component for crystallography or cryo-EM studies of the GINS complex.
• Western Blot Standard: Can serve as a positive control or size marker in immunoblot experiments targeting GINS3.
• Drug Discovery: May be used in screening assays targeting DNA replication factors as potential anti-cancer targets.

End-users are advised to validate the protein in their specific assay format. Aladdin Scientific technical support can assist with application-specific recommendations.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human GINS3 Protein are critical for maintaining activity and preventing degradation. General best-practice guidelines are provided below; always refer to the product-specific datasheet for authoritative conditions:

• Short-term Storage (≤1 month): Store at 4 °C if the protein will be used promptly.
• Long-term Storage: Aliquot and store at −20 °C or −80 °C to avoid repeated freeze–thaw cycles, which can cause protein aggregation and loss of activity.
• Freeze–Thaw Cycles: Minimize the number of freeze–thaw cycles; prepare single-use aliquots upon receipt.
• Carrier Protein: If the formulation does not contain a stabilizing carrier, consider adding BSA (0.1%) to diluted working stocks.
• Avoid: Repeated freeze–thaw, exposure to detergents incompatible with downstream assays, and prolonged incubation at room temperature.

Specific stability data and expiration information are provided on the product label and CoA.

Ordering Information and Technical Support

Recombinant Human GINS3 Protein is available exclusively through Aladdin Scientific under SKU rp368337. To place an order or request a quotation:

• Visit the Aladdin Scientific website and search for SKU rp368337 or product name Recombinant Human GINS3 Protein.
• Contact your regional Aladdin Scientific sales representative for bulk pricing, custom formulation inquiries, or institutional purchase orders.
• For technical questions regarding experimental setup, application suitability, or troubleshooting, reach out to the Aladdin Scientific technical support team.

Ensure your institution's procurement system is configured to accept Aladdin Scientific as an approved vendor. Certificate of Analysis, Safety Data Sheet (SDS), and product datasheet are available for download from the product page.

Frequently asked questions

What is the biological function of GINS3 (PSF3)?

GINS3, also known as PSF3, is a subunit of the tetrameric GINS complex (SLD5–PSF1–PSF2–PSF3), which is essential for eukaryotic DNA replication. Together with CDC45 and the MCM2–7 helicase, GINS3 contributes to the CMG holohelicase that unwinds duplex DNA at the replication fork, enabling coordinated leading- and lagging-strand synthesis.

What expression system is used to produce Recombinant Human GINS3 Protein (rp368337)?

The exact expression system for rp368337 is specified on the product datasheet and Certificate of Analysis available from Aladdin Scientific. Common recombinant expression platforms for GINS subunit proteins include E. coli and baculovirus/insect cell systems. Please download the latest datasheet from the product page or contact technical support for batch-specific details.

What applications is Recombinant Human GINS3 Protein suitable for?

Recombinant Human GINS3 Protein is suitable for protein–protein interaction assays (e.g., pull-down, co-IP), in vitro biochemical reconstitution of the GINS complex or CMG helicase, structural biology studies (crystallography, cryo-EM), Western blot positive controls, and drug discovery screening campaigns targeting DNA replication. Researchers should empirically validate the protein for each specific application.

How should Recombinant Human GINS3 Protein be stored to maintain stability?

For short-term use (within one month), store at 4 °C. For long-term storage, aliquot the protein into single-use volumes and store at −20 °C or −80 °C. Avoid repeated freeze–thaw cycles, as these can cause aggregation and loss of biological activity. Always refer to the product datasheet for formulation-specific storage recommendations.

How can I obtain a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) for rp368337?

The Certificate of Analysis, Safety Data Sheet, and product datasheet for Recombinant Human GINS3 Protein (rp368337) are available for download directly from the product page on the Aladdin Scientific website. For batch-specific CoA requests or additional documentation, contact Aladdin Scientific customer or technical support with your lot number.

References

1. Takayama Y, et al. GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes & Development. 2003.
2. Ilves I, et al. Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins. Molecular Cell. 2010.
3. Aladdin Scientific. Recombinant Human GINS3 Protein (rp368337) Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.