Lignin is a biopolymer with a three-dimensional network structure formed by the interconnection of three phenylpropane units via ether and carbon-carbon bonds. It is present in lignified tissues and primarily functions to harden cell walls by forming an interwoven network, serving as a major component of the secondary wall. Lignin is mainly located between cellulose fibers, providing compressive strength. In woody plants, lignin constitutes about 25% of the biomass and is the second most abundant organic compound in the world. In nature, lignin is often linked with cellulose and hemicellulose, forming a Lignin-Carbohydrate Complex (LCC). Consequently, it is currently not possible to isolate native lignin with its structure completely intact.
Principle:
The Lignin Staining Solution (Safranin Method) consists of Safranin Staining Solution and Glycerol Mounting Medium. It stains lignin using Safranin O, causing lignified cell walls to appear red or dark red. Other commonly used methods include the phloroglucinol method (Weisner staining), acidified aniline method, mixed staining method (Pianeze staining), and Mäule staining. Among these, the phloroglucinol method is the simplest and most frequently used microchemical technique for detecting lignin in plant cell walls. This reagent is for research use only, not suitable for clinical diagnosis or other purposes.
L1509228
| Component
| 50 mL | Storage
|
L1509228A
| Safranin Staining Solution
| 50 mL
| RT.
|
L1509228B
| Glycerol Mounting Medium
| 10 mL
| RT. |
Reagents, consumables and Equipments not provided
Glass slides, Cover slips, Microscope, Deionized water or Distilled water.
Procedure (For Reference Only)
Place the prepared sections into the Safranin Staining Solution and stain for 1 to 12 hours.
Rinse with distilled water for 2 to 5 minutes.
Add one drop of Glycerol Mounting Medium to mount the section. Observe and photograph under a microscope using visible light.
Staining Result
Lignified cell walls appear red. The color intensity corresponds to the degree of lignification, while non-lignified tissues remain colorless.
Precautions
The greater the degree of lignification, the deeper the color.
It is generally recommended to use free-hand sections or frozen sections to preserve the integrity of the sample contents.
The use of paraffin sections should be avoided as much as possible, as the complex paraffin embedding process may lead to lignin loss.
Safranin O staining for lignin is not absolute; with slightly prolonged staining time, almost all cell walls will take up the stain. This staining solution can be diluted with distilled water to achieve a light pink color against a white background.
After opening the reagent, please use it as soon as possible to prevent affecting subsequent experimental results.
For your safety and health, please wear a lab coat and disposable gloves during operation.
ZJ26F0333281