BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Room temperature Ships Normal Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
For isolation of Brucella spp.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Below is a generalized protocol framework for Liver Infusion Medium; consult your CoA/SDS and institutional SOPs for exact instructions.
A. Preparation of Liquid Medium (literature/general)
B. Preparation of Plates (literature/general)
C. Quality Control (literature/general) • Inspect for clarity, color, and absence of precipitate/contamination. • Measure pH of a representative sample. • Perform growth promotion testing with representative organisms relevant to your workflow (e.g., fastidious/anaerobic strains).
Note: Item-specific preparation parameters are Not specified for this item; refer to CoA/Spec Sheet.
This product has no intrinsic biological role as a single molecule; it functions as a nutrient matrix to support microbial metabolism (literature/general). • Metabolic support: Provides peptides, amino acids, minerals, and growth factors that feed central metabolic pathways (glycolysis, TCA cycle) and biosynthesis (nucleotides, proteins, cell wall components) in diverse microorganisms. • Growth factors: Tissue infusions can contribute heme/iron and complex lipids that benefit fastidious and anaerobic bacteria, improving respiration or fermentation efficiencies (literature/general). • Redox environment: When combined with reducing agents and anaerobic handling, the medium can help maintain low redox potential conducive to strict anaerobes (literature/general). • Phenotypic expression: Rich, undefined nutrients often enhance recovery of stressed or injured cells and may modulate expression of virulence or stress-response factors compared with minimal media (organism-dependent; literature/general). • Research context: Useful for resuscitation, colony isolation (when solidified with agar), and preparation of inocula for downstream assays (e.g., antimicrobial susceptibility testing, biochemical profiling) after appropriate standardization.
This product is not a dedicated buffering agent or defined buffer system. Any buffering capacity arises from intrinsic components of the formulation and is not standardized. • For experiments requiring precise pH control, use a defined buffer system (e.g., phosphate, HEPES, MOPS) and, if appropriate, combine with the medium according to validated microbiological protocols (literature/general). • Item-specific buffer parameters (pH range, capacity): Not specified for this item; refer to CoA/Spec Sheet.
While complex animal-derived media like Liver Infusion Medium can be highly effective for fastidious microbes, laboratories seeking to reduce animal origin content or environmental impact may consider alternatives (literature/general):
Comparison (literature/general): • Liver Infusion Medium (animal-derived): Excellent for certain fastidious/anaerobic organisms due to tissue-derived growth factors; variability and animal-origin concerns may apply. • Brain Heart Infusion (BHI, animal-derived): Broad-spectrum rich medium; widely standardized and GPT-supported; still animal-origin. • Vegetable/yeast/soy peptone-based media (animal-free): Reduce animal-origin risks, often suitable for routine cultivation; may be less effective for specific fastidious taxa relying on heme/iron/complex lipids. • Chemically defined media: Highest reproducibility and traceability; may require organism-specific optimization and supplements; reduced waste complexity.
Sustainability practices: • Prepare only required volumes to minimize waste; use reusable glassware where appropriate. • Validate animal-free alternatives for non-fastidious organisms to reduce reliance on animal derivatives. • Employ energy-efficient sterilization cycles (validated shorter cycles or lower loads) and consolidate autoclave runs to cut energy/water usage.
No therapeutic or clinical diagnostic claims are made. For research use only (item-specific).
Relevant roles in pharmaceutical/industrial microbiology (literature/general): • Growth promotion and recovery of fastidious organisms in environmental monitoring and contamination investigations when tissue-derived factors are advantageous. • Pre-enrichment/resuscitation of stressed bioburden in process samples prior to testing on selective or differential media. • Method development: Evaluating organism-specific media requirements; comparing animal-derived vs animal-free media for QC workflows. • Not a compendial replacement: Pharmacopeial chapters (e.g., USP/EP) commonly prescribe BHI, TSB, or others for compendial tests. Liver Infusion Medium may be used for non-compendial research, isolate maintenance, or exploratory method development where appropriate and justified. • Documentation: Maintain lot traceability, GPT records, and sterilization logs consistent with quality systems; verify that use aligns with internal SOPs and regulatory expectations.
Item-specific physical constants are generally not defined for complex culture media. • Appearance (as supplied): Not specified for this item; refer to CoA/Spec Sheet. • Solubility: Freely soluble in purified/deionized water to form a nutrient broth (literature/general); complete dissolution facilitated by gentle heating and stirring prior to sterilization. • pH (prepared medium): Not specified for this item; refer to CoA/Spec Sheet. (Literature: complex media typically target near-neutral pH after sterilization; users often verify and adjust prior to autoclaving.) • Density, melting/boiling point, refractive index, logP, pKa: Not applicable/Not defined for complex mixtures; Not specified for this item; refer to CoA/Spec Sheet. • Optical properties: Prepared broth is typically clear to slightly opalescent; any excessive turbidity or precipitate may indicate improper dissolution or contamination (literature/general). • Hygroscopicity: Many dehydrated media are moisture-sensitive; exposure to humidity can cause clumping and variability in performance (literature/general). Keep container tightly closed with desiccant. • Thermal behavior: Dehydrated powders do not have a single melting point; prepared media are commonly sterilized by autoclaving (121 °C) or filtration depending on heat stability of supplements (literature/general).
• Grade: BioReagent (item-specific). BioReagent grade typically indicates suitability for general biological research, with attention to lot-to-lot consistency and contaminant control appropriate for lab workflows. It does not imply sterility of the powder as supplied and is distinct from cell-culture or clinical diagnostic grades. • What this means in practice:
This product is a biological culture medium, not a reagent for synthetic chemical reactions. Accordingly, typical organic reaction families (e.g., Grignard, cross-coupling) are not applicable.
Applicable laboratory uses (literature/general): • Cultivation and maintenance of fastidious bacteria that respond to organ tissue infusions, including certain anaerobes (e.g., Clostridium spp.) and microaerophiles. • Base matrix for preparing enriched or selective formulations by addition of carbohydrates, reducing agents, blood/hematin, or antibiotics. • Pre-enrichment of low-level contaminants in environmental, food, or pharmaceutical microbiology workflows where rich, complex nutrients improve recovery. • Anaerobic culture workflows: Combine with reducing agents and use in anaerobic jars/chambers; pre-reduce media where required to enhance recovery of strict anaerobes. • As a component in multi-step isolation pipelines: initial resuscitation in rich medium followed by subculture to differential/selective media.
Practical tips (literature/general): • Dissolve completely with gentle heat and stirring; adjust pH (if protocol requires) before sterilization. • Autoclave according to validated cycle; cool to dispensing temperature before adding heat-labile supplements using aseptic technique. • Perform growth promotion testing with representative organisms to verify lot suitability and sterilization efficacy.
Chemical reaction conditions are not applicable. The following are microbiological preparation and culture conditions provided as general literature guidance; adjust to organism and protocol.
Preparation (literature/general): • Reconstitution: Dissolve the dehydrated powder in purified water at the concentration recommended by your protocol or CoA. Stir with gentle heat until fully dissolved; avoid overheating or Maillard browning. • pH: Verify and adjust prior to sterilization if your SOP specifies a target pH for the organism of interest. • Sterilization: Autoclave using a validated cycle (e.g., 121 °C for an appropriate hold time) or filter-sterilize heat-labile supplements separately and add aseptically after cooling. • Solid media: To prepare plates, dissolve an appropriate amount of agar with the medium, sterilize, cool to dispensing temperature, then pour plates aseptically.
Culture (literature/general): • Inoculation: Use aseptic technique; for anaerobes, pre-reduce medium and handle in an anaerobic chamber/jar with suitable gas mix and indicators. • Incubation: Temperature, atmosphere, and duration depend on the target organism; many mesophilic bacteria grow between ~30–37 °C, while others require organism-specific conditions. • Quality control: Perform growth promotion testing with representative strains to confirm performance of each lot and sterilization cycle.
• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS for authoritative safety information. • General hazards (literature/general): Dehydrated nutrient media may cause mechanical irritation to eyes/respiratory tract as nuisance dust; not intended for human or animal consumption. Once hydrated and inoculated, media become biohazardous due to microbial growth potential. • PPE: Laboratory coat, safety glasses, and appropriate gloves. Use dust control (avoid aerosolization); handle powders in a fume hood or biosafety cabinet if dust exposure or bioaerosol generation is possible. • Incompatibilities: Avoid moisture uptake; store away from oxidizing agents and strong acids/bases that could degrade nutrients (literature/general). Do not mix with disinfectants/biocides if the medium is intended for growth support. • First aid (overview; defer to SDS): If inhaled, move to fresh air; if eye/skin contact occurs, rinse with water. Seek medical evaluation for persistent irritation. If ingested, rinse mouth and seek advice. • Microbiological safety: Treat used/contaminated media as infectious waste. Decontaminate by autoclaving before disposal. Employ aseptic technique to prevent unintended organism proliferation. • Fire safety: Organic content may be combustible; use standard extinguishing media suitable for surrounding materials (literature/general).
• Primary solvent: Water. Liver Infusion Medium is designed to be reconstituted in purified/deionized water; organic solvents are not applicable. • Water quality (literature/general): Use freshly produced, low-conductivity, low-TOC water. Avoid residual disinfectants (e.g., chlorine, ozone) and chelators that can inactivate growth factors. • Miscibility: Upon dissolution in water, forms an aqueous broth compatible with downstream sterilization by autoclaving or sterile filtration of heat-labile supplements. • Ionic strength/osmolarity: Determined by the formulation and any added salts/sugars; verify as needed for sensitive organisms (literature/general). Item-specific values: Not specified for this item; refer to CoA/Spec Sheet. • When to choose this medium vs alternatives (literature/general):
• Storage (as supplied): Room temperature (item-specific). Ship: Normal (item-specific). Keep container tightly closed in a cool, dry place; protect from humidity. Use desiccant where possible to prevent caking and performance drift. • Shelf life: Not specified for this item; refer to CoA/Spec Sheet. Avoid repeated opening in humid environments. • Reconstitution: Dissolve in purified/deionized water at the concentration required by your protocol. Mix with gentle heat until fully dissolved. Item-specific concentration and target pH: Not specified for this item; refer to CoA/Spec Sheet. • Sterilization: Autoclave according to validated parameters. Alternatively, prepare sterile stock by filtration for heat-labile supplements added post-autoclave (literature/general). • Prepared medium storage (literature/general): Store sterile broth or plates at 2–8 °C, protected from light and dehydration. Use within a reasonable period as defined by your SOP and GPT schedule. Discard if contamination, color change, precipitate, or dryness is observed. • Freeze-thaw guidance: Freezing of prepared complex media is generally not recommended due to potential component precipitation and performance changes (literature/general). If freezing is unavoidable, validate impact on target organisms. • Research Use Note: For research use only (item-specific). Not for human or animal therapeutic or diagnostic use.
• Item type: complex, undefined microbiological culture medium derived from animal tissue infusion (liver) with added nutrients (literature/general). It is not a single chemical entity. • SKU: L1528663; Product name: Liver Infusion Medium. • CAS, InChIKey, SMILES: Not applicable for complex media. • Molecular formula / Molecular weight: Not applicable to complex mixtures; individual constituents vary (peptides, amino acids, carbohydrates, minerals). Item-specific values: Not specified for this item; refer to CoA/Spec Sheet. • Structural features: As a dehydrated nutrient blend, it does not possess a discrete 2D/3D molecular structure. Typical components (literature) include: liver infusion solids (source of peptides, amino acids, heme derivatives), peptones/tryptones, salts, and optional carbohydrate sources. Exact composition is supplier-specific. • Identity confirmation (literature practice): Verified by label claim and growth promotion performance with representative microorganisms rather than by spectroscopic identity; pH and clarity of prepared medium are commonly checked as functional identifiers.
Not applicable. Liver Infusion Medium is a biological culture medium, not a functionalized chemical building block. It is not used as a reagent or substrate in organic synthesis.
For chemists seeking chemically defined nutrients or additives to control microbial bioprocesses, consider supplementing cultures with defined amino acids, vitamins, trace metals, or carbon sources according to organism-specific literature.
Not applicable. This product is not an antibody, oligonucleotide, or affinity reagent. It does not possess target-binding specificity. There are no clone, isotype, antigen, or species-reactivity details for this item.
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View BioReagent grade guide →
