Lysine Medium (LYS) - BioReagent

Cat. No.: L1528596

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Overview

For isolation of wild Saccharomyces cerevisiae

Specifications

Specifications & Purity

BioReagent

Storage

Room temperature

Shipped In

Normal

Grade

BioReagent

Product Line

biological_products

Application Protocols

Item-specific tested applications

• Not specified for this item; refer to CoA/Spec Sheet.

General protocol concepts (literature; choose those matching your formulation)

• Lysine decarboxylase test (broth): Inoculate a fresh culture into lysine decarboxylase broth, overlay with sterile mineral oil to create anaerobic conditions, incubate (e.g., 24–48 h at 35–37 °C). Interpret color change via pH indicator: alkaline shift indicates decarboxylation (organism-dependent controls required).
• Lysine iron agar (if applicable): Streak stab-and-streak method, incubate 18–24 h at 35–37 °C. Read slant/butt reactions for lysine decarboxylation/deamination and H2S production per standard keys.
• Yeast selection (−Lys or +Lys defined media): Prepare defined base, add or omit lysine as required. Inoculate auxotrophic strains and incubate at the organism’s optimal temperature, monitoring growth to confirm selection or complementation.

Important

• Exact composition, incubation conditions, and readouts for Lysine Medium (LYS) are Not specified for this item; refer to CoA/Spec Sheet and validate with controls.

Biological Roles

General context (literature)

• Lysine is an essential, cationic amino acid (pKa of ε-NH3+ ~10.5, literature) incorporated into proteins and central to metabolism (e.g., saccharopine and pipecolate pathways in various organisms). Media that modulate lysine availability are powerful tools for probing amino acid transport, catabolism (e.g., decarboxylation to cadaverine), and regulation of biosynthetic pathways.
• In microbes, lysine decarboxylation elevates pH and can contribute to acid stress responses; detection of this activity underpins classic biochemical identification schemes.
• In yeast genetics, lysine auxotrophy is widely used for selection; omission or complementation of lysine in defined media controls plasmid or chromosomal marker maintenance.

Item-specific note

• The exact biological role targeted by this Lysine Medium (LYS) depends on its formulation (presence/absence of lysine, indicators, and other nutrients). These details are Not specified for this item; refer to CoA/Spec Sheet.

Use constraints

• For research use only (per Product Data). Not for human or animal therapeutic or diagnostic use.

Buffer Applications

Not typically applicable

• Lysine Medium (LYS) is a culture medium, not a dedicated buffering system. While prepared media possess a defined pH and may contain buffering salts, they are not used as analytical buffers.

General note (literature)

• If your application requires precise buffering independent of nutrient composition (e.g., enzyme assays), prepare a separate buffer and, if appropriate, supplement with defined nutrients or amino acids. The buffering components and pH range for this specific medium are Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

Context

• As a dehydrated aqueous medium, this product already avoids volatile organic solvents typical of synthetic chemistry. Greener practice focuses on packaging, sterilization energy, and waste minimization.

Greener practice options (general)

• Concentrate and prepare only the volume needed to reduce autoclave energy and media waste.
• Use energy-efficient sterilization: validate membrane filtration in place of autoclaving for heat-labile components; for agar-containing media, autoclave batchwise and keep at 45–50 °C in a water bath to reduce repeated heat cycles.
• Choose reusable glassware where feasible; if using single-use plastics (bottles, plates), segregate and autoclave before recycling streams where permitted.
• Prefer powdered media (as supplied) over ready-to-use liquids to cut shipping mass/volume and lower transport emissions.

Comparison (literature; qualitative)

• Dehydrated custom/defined media vs. rich complex media: defined media can lower downstream nitrogenous waste and improve reproducibility; complex media may generate more variable effluents. Trade-off: defined media may require more supplements and QC.

Item-specific note

• No alternative SKU information provided; composition details are Not specified for this item; refer to CoA/Spec Sheet.

Pharmaceutical Uses

Not a pharmaceutical excipient

• This product is a laboratory culture medium for research use only (per Product Data). It is not intended for human or veterinary formulation.

Manufacturing/quality context (general)

• In pharmaceutical microbiology labs, lysine-focused media can support identification, selection, or environmental monitoring workflows. Any such use must follow compendial methods (e.g., pharmacopeial microbiology chapters) and validated internal SOPs.

Item-specific status

• No pharmacopoeial alignment, excipient status, or GMP suitability is provided. Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific facts (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Storage conditions: Room temperature (per Product Data).

General properties of dehydrated culture media (literature)

• Typical physical form: free-flowing, hygroscopic powder or granules designed for rapid dissolution in purified water.
• Solubility: formulated for complete dissolution in water; insoluble in organic solvents.
• pH (prepared medium): application-dependent; manufacturers typically specify a target pH at 25 °C measured after reconstitution and sterilization (e.g., many lysine-associated broths/agar recipes target pH ~6–7.5; literature/general guidance only—consult the product’s CoA/Spec Sheet for the exact value).
• Color: varies with composition and presence/absence of pH or decarboxylase indicators (literature/general).

Not applicable for mixtures

• Boiling point, melting point, density, refractive index, logP, and other pure-compound constants are not applicable to complex media blends.

Quality and Grades

Item-specific facts (from Product Data)

• Grade/Purity: BioReagent.

What BioReagent grade means (general)

• BioReagent grade indicates suitability for common biochemical, cell culture, and microbiological workflows, focusing on lot-to-lot performance, low bioburden, and consistent composition rather than ultra-trace inorganic/organic impurity specifications.
• For culture media, key QC attributes typically include: appearance, solubility/clarity after preparation, pH of the prepared medium, sterility (preparation-dependent), and growth performance with designated control organisms or genetic selection markers (literature/general).

Item-specific notes

• Exact composition, indicator content, and performance criteria for Lysine Medium (LYS) are Not specified for this item; refer to CoA/Spec Sheet. Use the lot-specific Certificate of Analysis to verify performance tests, target pH, and any supplementary acceptance criteria.
• Shipped at ambient temperature and stored at room temperature per Product Data—consistent with dehydrated media stability when kept sealed and dry.

Reaction and Applications

Scope of use

• This is a culture medium for life science research; it is not a reagent for chemical synthesis. “Reactions” in the synthetic-chemistry sense do not apply. Instead, consider biological applications where lysine availability or metabolism is probed.

Literature/general applications of lysine-focused media

• Microbiological differentiation: Lysine decarboxylase/lysine utilization tests employ specialized lysine-containing broths to detect decarboxylase activity in Enterobacteriaceae, often monitored by pH color indicators under anaerobic overlay conditions.
• Differential/indicator agars (e.g., lysine iron systems): Formulations can differentiate organisms based on lysine decarboxylation/deamination and H2S production patterns (general literature).
• Yeast genetics and selection: “Lysine dropout” or “−Lys” media omit lysine to select for auxotrophic markers (e.g., LYS2/LYS5) or plasmid maintenance in S. cerevisiae. Conversely, lysine-supplemented media can rescue lysine auxotrophs and standardize growth.
• Metabolic studies: Defined media with controlled lysine concentration are used to study amino acid transport, regulation, and metabolic flux.

Item-specific note

• The precise formulation and intended test paradigm for Lysine Medium (LYS) are Not specified for this item; refer to CoA/Spec Sheet. Validate with appropriate positive/negative control strains or lines relevant to your workflow.

Reaction Conditions

Not applicable in the synthetic chemistry sense

• There are no catalyst/solvent/temperature parameters to report for chemical reactions.

General preparation conditions for media (literature; procedural guidance only)

• Reconstitution: Dissolve the specified mass of dehydrated medium per liter of purified water with gentle heating and stirring to aid dissolution (exact mass and target pH are Not specified for this item; refer to CoA/Spec Sheet).
• Sterilization: Autoclave at 121 °C for 15–20 min for heat-stable formulations. For heat-labile supplements (e.g., certain amino acids, antibiotics, vitamins), sterile-filter and add aseptically post-autoclave.
• Agar media: If the formulation includes agar, dispense and cool to 45–50 °C before pouring plates.
• Quality checks: Confirm pH at room temperature, examine clarity/precipitate, and verify performance using appropriate positive/negative control organisms or strains relevant to lysine metabolism/auxotrophy. These steps are general; consult the product’s CoA/Spec Sheet for exact instructions.

Safety and Handling

Item-specific facts (from Product Data)

• GHS signal word / pictograms / hazard statements: Not specified for this item; refer to the SDS.
• Shipping: Normal (per Product Data). Storage: Room temperature.

General guidance (literature; defer to SDS for authoritative instructions)

• Expected hazards: Dehydrated media powders can cause mechanical/respiratory irritation if dust is generated; avoid inhalation and contact with eyes. Not typically classified as hazardous, but classification depends on exact formulation.
• PPE: laboratory coat, safety glasses, and suitable gloves. Use dust control (weighing in fume hood or with local exhaust) to minimize airborne particulates.
• Incompatibilities: Store away from strong oxidizers and moisture. Hygroscopic—keep tightly sealed with desiccant to prevent caking and degradation.
• Microbiological risk: Once prepared, media can support microbial growth. Handle prepared medium using aseptic technique; treat spent media and cultures as biohazardous and autoclave prior to disposal in accordance with institutional biosafety procedures.
• First aid (overview): If inhaled—move to fresh air; if eye/skin contact—rinse with water; if ingested—rinse mouth. Seek medical attention if symptoms persist.
• Fire safety: Organic components are combustible; in a fire use standard extinguishing media appropriate for surrounding materials. Always consult the SDS and institutional EHS guidance before use.

Solvent Selection

Applicability

• This product is a dehydrated medium intended for aqueous preparation. Organic solvent selection is not relevant.

General preparation solvent guidance (literature)

• Use purified, deionized water (Type II or better) for reconstitution. For cell/microbiology media, 0.2 µm filtration or autoclaving is typically used to achieve sterility; choose the sterilization method compatible with the medium’s heat sensitivity (consult CoA/Spec Sheet).
• If indicators or heat-labile supplements (e.g., antibiotics, amino acids, vitamins) are required by the application, add them aseptically after autoclaving and cooling to ~45–55 °C (agar) or room temperature (broth).

Comparison among water sources (general)

• Distilled or RO water: acceptable for most media; ensure conductivity and TOC within institutional limits.
• Type I ultrapure water: minimizes background contamination for sensitive biochemical assays but is not always necessary for routine microbiology.

Note

• Exact reconstitution volume and target pH are Not specified for this item; refer to CoA/Spec Sheet.

Storage and Reconstitution

Item-specific facts (from Product Data)

• Storage conditions (powder): Room temperature. Shipped at ambient temperature.

General best practices (literature)

• Keep container tightly sealed in a cool, dry place away from humidity; consider secondary containment with desiccant. Avoid repeated exposure to air to limit moisture uptake and caking.
• Reconstitution: Dissolve the specified mass per liter of purified water (exact mass Not specified for this item; refer to CoA/Spec Sheet). Adjust pH only if directed by the product specification. Bring to final volume after components fully dissolve.
• Sterilization: Autoclave at 121 °C for 15–20 minutes for heat-stable media. For heat-labile supplements, sterile-filter and add aseptically after cooling (agar: 45–50 °C; broth: room temperature).
• Shelf life: Dehydrated media are generally stable for months to years when sealed and dry; prepared media shelf life depends on composition and storage (refrigerated 2–8 °C in the dark is common for plates and broths). Exact stability for this item is Not specified; refer to CoA/Spec Sheet.
• Freeze–thaw: Not applicable to dehydrated powder; avoid freezing prepared agar plates (can cause cracking) unless validated for your application.

Structure and Identity

Item-specific facts (from Product Data)

• Product name: Lysine Medium (LYS); SKU: L1528596
• Grade: BioReagent
• CAS: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES / InChI / InChIKey: Not applicable to a complex medium; Not specified for this item; refer to CoA/Spec Sheet.

General notes (literature)

• Lysine Medium (LYS) denotes a formulated, dehydrated culture medium blend rather than a single defined chemical entity. Such media may contain amino acids (notably L-lysine), carbon and nitrogen sources, salts, vitamins, and indicators, depending on the intended microbiological/genetic application.
• Structural identifiers (SMILES, InChI) are not defined for mixtures; identity is established by composition specification, performance criteria (growth/biochemical response of control strains), and QC parameters (pH, appearance, sterility).

Synthetic Utility

Not applicable

• Lysine Medium (LYS) is not a building block or reagent for organic synthesis. It serves as a nutrient medium for biological systems. For chemical transformations involving lysine or derivatives (e.g., peptide coupling, protection/deprotection), use reagent-grade amino acids and peptide synthesis reagents rather than culture media.

Target Specificity

Not applicable

• This product is a culture medium, not an antibody, probe, or targeted affinity reagent. There is no antigen/epitope or species reactivity to report.

Frequently Asked Questions

How should this product be stored?

Store at room temperature.